Field Performance of Transgenic Potato, with Resistance to Colorado Potato Beetle and Viruses

Duncan, David R.; Hammond, David; Zalewski, Jim; Cudnohufsky, John; Kaniewski, Wojciech; Thornton, Mike; Bookout, Jeffrey T.; Lavrik, Paul B.; Rogan, Glennon J.; Feldman-Riebe, Jennifer

doi:10.21273/hortsci.37.2.275

Cited by 22 publications

(8 citation statements)

References 1 publication

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“…One resistant line expressing the PVY coat protein gene combined with the Bt insecticidal protein was commercialized by the Monsanto subsidiary company NatureMark. This variety, ‘Newleaf Y’, was commercially planted in the late 1990s and early 2000s (Borlaug, 2000; Duncan et al. , 2002).…”

Section: Introductionmentioning

confidence: 99%

Engineering virus resistance using a modified potato gene

Cavatorta

Perez

Gray

et al. 2011

Plant Biotechnology Journal

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Summary Natural mutations in translation initiation factor eIF4E confer resistance to potyviruses in many plant species. Potato is a staple food crop plagued by several potyviruses, yet to date no known eIF4E‐mediated resistance genes have been identified. In this study, we demonstrate that transgenic expression of the pvr12 gene from pepper confers resistance to Potato virus Y (PVY) in potato. We then use this information to convert the susceptible potato ortholog of this allele into a de novo allele for resistance to PVY using site‐directed mutagenesis. Potato plants overexpressing the mutated potato allele are resistant to virus infection. Resistant lines expressed high levels of eIF4E mRNA and protein. The resistant plants showed growth similar to untransformed controls and produced phenotypically similar tubers. This technique disrupts a key step in the viral infection process and may potentially be used to engineer virus resistance in a number of economically important plant–viral pathosystems. Furthermore, the general public may be more amenable to the ‘intragenic’ nature of this approach because the transferred coding region is modified from a gene in the target crop rather than from a distant species.

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Section: Introductionmentioning

confidence: 99%

Engineering virus resistance using a modified potato gene

Cavatorta

Perez

Gray

et al. 2011

Plant Biotechnology Journal

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“…The genes used to date have mainly been ones that code for proteins which are toxic to pests and pathogens, ones whose expression interferes with virus multiplication in host cells and ones that code for key enzymes in biochemical pathways in other organisms, often, but not always other plant species. Genes which encode FCry_ proteins from the bacterium Bacillus thuringiensis confer resistance to Colorado potato beetle (CPB, Leptinotarsa decemlineata Say) and potato tuber moth (Phthorimaea operculella) (Mohammed et al, 2000;Duncan et al, 2002;Davidson et al, 2004) and genes which encode cysteine proteinase inhibitors (cystatins) confer resistance to potato cyst nematodes (Globodera rostochiensis and G. pallida) (Urwin et al, 2003). Replicase and coat protein genes from Potato Leafroll Luteovirus and Potato Y Potyvirus, respectively, provide resistance to these viruses (Duncan et al, 2002).…”

Section: Genetically Modified Potatoesmentioning

confidence: 99%

“…Genes which encode FCry_ proteins from the bacterium Bacillus thuringiensis confer resistance to Colorado potato beetle (CPB, Leptinotarsa decemlineata Say) and potato tuber moth (Phthorimaea operculella) (Mohammed et al, 2000;Duncan et al, 2002;Davidson et al, 2004) and genes which encode cysteine proteinase inhibitors (cystatins) confer resistance to potato cyst nematodes (Globodera rostochiensis and G. pallida) (Urwin et al, 2003). Replicase and coat protein genes from Potato Leafroll Luteovirus and Potato Y Potyvirus, respectively, provide resistance to these viruses (Duncan et al, 2002). The gene chly encoding a chicken lysozyme enzyme enhances resistance to blackleg and soft rot caused by infection with Erwinia carotovora subsp.…”

Section: Genetically Modified Potatoesmentioning

confidence: 99%

Genetic Resources (Including Wild and Cultivated Solanum Species) and Progress in their Utilisation in Potato Breeding

Bradshaw¹,

Bryan²,

Ramsay³

2006

Potato Research

146

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The genetic resources available for the improvement of the cultivated potato (Solanum tuberosum) are reviewed along with progress in their utilisation. The conclusions are as follows. The wild and cultivated species of potato have been utilised in potato breeding to good effect, but only a very small sample of the available biodiversity has been exploited. New knowledge and technology will open possibilities for much greater use of these genetic resources in breeding. The strategy for utilising the cultivars native to Latin America will either be the introgression of desirable genes or the direct use of parents from improved populations, depending on how far modern S. tuberosum cultivars have genetically diverged from them and the extent to which S. tuberosum cultivars have been improved in the process. Molecular marker-assisted selection will be used for faster introgression of desirable genes from wild species, and the possibility exists of moving genes directly from wild species to cultivated potato with transgenic methods. New cultivars will continue to come from crosses between pairs of parents with complementary features but adapted to local growing conditions. However, increasingly these parents will possess desirable genes which have been introgressed from wild species and may also be from complementary groups of cultivated germplasm to exploit hybrid vigour. Successful cultivars may be genetically modified, if consumers see benefits in the use of the technology, to introduce genes not present in cultivated potatoes and their wild relatives to achieve novel biochemistry and further desirable improvements.

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“…Advances in molecular biology and biotechnology have led to the successful development and commercial release in the United States of several virus-resistant crops, including papaya resistant to Papaya ringspot virus (Gonsalves, 1998), potato resistant to Potato virus Y (Smith et al, 1995) and Potato leafroll virus (Duncan et al, 2002), and a squash cultivar resistant to Cucumber mosaic virus, Watermelon mosaic virus-2 and Zucchini yellow mosaic virus (Fuchs et al, 1998). All of these viruses have RNA genomes, and all plants are resistant due to RNA silencing (also referred to as posttranscriptional gene silencing) of the transgene.…”

Section: Introductionmentioning

confidence: 99%

Transgenic Banana Plants Resistant to Banana Bunchy Top Virus Infection

Borth¹,

Pérez²,

Cheah³

et al. 2011

Acta Hortic.

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Embryogenic cell suspensions (ECS) initiated from immature male flowers of banana cultivar 'Dwarf Brazilian' (AAB, Pome subgroup) were transformed using Agrobacterium tumefaciens containing one of four constructs derived from the replicase-associated protein (Rep) gene of the Hawaiian isolate of Banana bunchy top virus (BBTV). Each construct was engineered under control of a double CaMV 35S promoter and the AMV enchancer sequence in the binary plasmid pBI121. Constructs were transferred into A. tumefaciens strain AGL0 and used to transform banana ECS. Plantlets that survived antibiotic selection were acclimated to greenhouse conditions and challenged with viruliferous banana aphids (Pentalonia nigronervosa). Ten adult or late instar aphids were allowed to feed for 2-4 weeks on test plants. All test plants were kept in the greenhouse and monitored for symptom expression for a period of 6 months. Control plants transformed with empty vector pBI121 only were included in all tests. A total of 270 test plants and 63 control plants were screened for BBTV resistance using this approach. One of 32 test plants transformed with the M1 (mutant Rep gene) construct, 5 of 74 test plants transformed with the AS1 (antisense Rep gene) construct, 5 of 38 test plants transformed with the PR1 (partial Rep gene) construct, and 10 of 126 test plants transformed with the R/PR1 (full-length Rep gene fused to antisepses partial Rep gene) construct were found to be resistant to BBTV challenge and showed no bunchy top symptoms. All of the control plants became infected with BBTV under these experimental conditions. Plants that survived BBTV challenge were analysed by quantitative PCR (per) and Southern hybridisations to determine the number of transgenes that were present in their genomes. Results from these analyses indicated that the resistant plants contained from 2 to more than 9 copies of the NPTII (kanamycin resistance) transgene carried on the pBI121 plasmid.

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Field Performance of Transgenic Potato, with Resistance to Colorado Potato Beetle and Viruses

Cited by 22 publications

References 1 publication

Engineering virus resistance using a modified potato gene

Engineering virus resistance using a modified potato gene

Genetic Resources (Including Wild and Cultivated Solanum Species) and Progress in their Utilisation in Potato Breeding

Transgenic Banana Plants Resistant to Banana Bunchy Top Virus Infection

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