Field-Portable Microfluidics-Based Imaging Flow Cytometer

Jagannadh, Veerendra Kalyan; Murthy, Rashmi Sreeramachandra; Srinivasan, Rajesh; Gorthi, Sai Siva

doi:10.1109/jlt.2015.2412654

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…However, adapting current single-cell technologies into a portable format holds its own set of unique challenges. Most of the existing literature surrounding such technologies still report separate sample enrichment or staining steps prior to cell analysis [106][107][108]; such additional preparatory steps increase assay complexity, which may not be desirable in a POC setting [109]. While a gamut of existing microfluidic technology has already been established for sample purification as well as for reagent addition and mixing, integrating the various modules into a single platform is typically not a trivial process [110].…”

Section: Toward Point-of-care Applicationsmentioning

confidence: 99%

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Lin

Tay

Lu³

et al. 2020

Cells

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The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host–pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.

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Section: Toward Point-of-care Applicationsmentioning

confidence: 99%

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Lin

Tay

Lu³

et al. 2020

Cells

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“…Microfluidic flow cytometers have now been made compatible with cellphone cameras used as their imaging sensors, 4,5 thereby increasing their affordability and portability. However, cellphone cameras have lower frame rates than commonly needed to capture rapidly flowing cells.…”

Section: Introductionmentioning

confidence: 99%

Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry

Chaturvedi

Gorthi

2017

SLAS Technology

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Portable microfluidic diagnostic devices, including flow cytometers, are being developed for point-of-care settings, especially in conjunction with inexpensive imaging devices such as mobile phone cameras. However, two pervasive drawbacks of these have been the lack of automated sample preparation processes and cells settling out of sample suspensions, leading to inaccurate results. We report an automated blood sample preparation unit (ABSPU) to prevent blood samples from settling in a reservoir during loading of samples in flow cytometers. This apparatus automates the preanalytical steps of dilution and staining of blood cells prior to microfluidic loading. It employs an assembly with a miniature vibration motor to drive turbulence in a sample reservoir. To validate performance of this system, we present experimental evidence demonstrating prevention of blood cell settling, cell integrity, and staining of cells prior to flow cytometric analysis. This setup is further integrated with a microfluidic imaging flow cytometer to investigate cell count variability. With no need for prior sample preparation, a drop of whole blood can be directly introduced to the setup without premixing with buffers manually. Our results show that integration of this assembly with microfluidic analysis provides a competent automation tool for low-cost point-of-care blood-based diagnostics.

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“…Due to its advantages of high throughput and multiparameter extraction, FCM has been widely used in immunology, biochemistry, biology, oncology, hematology, etc. , Generally, single-pixel photodiode can be used for sensing scattered signal from label-free cells or fluorescence signal stimulated from stained cells to retrieve the size and number information in a typical FCM system . By integrating the flow path into a microfluidic chip, fast analysis of cells can be realized. − However, this conventional FCM technique is incapable of observing the morphology of cells which is a critical index of H. pluvialis culturing. Therefore, imaging capability is a necessity for simultaneous measurement of morphology and size of the algal cells.…”

mentioning

confidence: 99%

Growth Characteristic Analysis of Haematococcus pluvialis in a Microfluidic Chip Using Digital in-Line Holographic Flow Cytometry

et al. 2022

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In order to obtain high yield of astaxanthin, a high-value compound with ultrastrong antioxidant capacity, it is necessary to identify the growth characteristics (biomass, morphology, and size) of Haematococcus pluvialis. The current detection methods have the disadvantages of labor-consuming operation or complicated measurement system. It is an urgent need to explore a simple and cost-effective method for the detection of H. pluvialis with large size distribution during its growth period. In this work, a digital in-line holographic flow cytometry using a linear array sensor is proposed to measure the growth characteristics of H. pluvialis in a two-dimensional (2-D) hydrodynamic focusing microfluidic chip. Based on the modified angular spectrum method, the distorting holograms caused by the asynchrony of sample flow velocity and acquisition speed of the linear array sensor were rectified and reconstructed. In addition, the depth-of-focus of the imaging system were digitally extended to cover the entire depth of the microfluidic channel for optimized imaging quality. We have utilized the proposed method to statistically investigate the biomass, morphology and size of H. pluvialis under different culture conditions and growth durations.

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Field-Portable Microfluidics-Based Imaging Flow Cytometer

Cited by 3 publications

References 17 publications

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

The Role of Single-Cell Technology in the Study and Control of Infectious Diseases

Automated Blood Sample Preparation Unit (ABSPU) for Portable Microfluidic Flow Cytometry

Growth Characteristic Analysis of Haematococcus pluvialis in a Microfluidic Chip Using Digital in-Line Holographic Flow Cytometry

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