Field-Related Mathematical Model to Predict and Reduce Reservoir Souring

Sunde, Egil; Thorstenson, Tore; Torsvik, Terje; Vaag, J.E.; Espedal, Magne S.

doi:10.2118/25197-ms

Cited by 42 publications

(18 citation statements)

References 6 publications

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“…It is now widely accepted that the reduction of sulfate by SRB is one of the most significant mechanisms of H 2 S production in reservoir souring (Ligthelm et al , 1991; Sunde et al , 1993). However, occasionally, oil reservoir souring has also been detected in the reservoirs during primary recovery (Vance & Thrasher, 2005).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR

Agrawal

Lal

2009

FEMS Microbiology Ecology

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Sulfate-reducing bacteria (SRB) pose a serious problem to offshore oil industries by producing sulfide, which is highly reactive, corrosive and toxic. The dissimilatory sulfite reductase (dsr) gene encodes for enzyme dissimilatory sulfite reductase and catalyzes the conversion of sulfite to sulfide. Because this gene is required by all sulfate reducers, it is a potential candidate as a functional marker. Denaturing gradient gel electrophoresis fingerprints revealed the presence of considerable genetic diversity in the DNA extracts achieved from production water collected from various oil fields. A quantitative PCR (qPCR) assay was developed for rapid and accurate detection of dsrB in oil field samples. A standard curve was prepared based on a plasmid containing the appropriate dsrB fragment from Desulfomicrobium norvegicum. The quantification range of this assay was six orders of magnitude, from 4.5 x 10(7) to 4.5 x 10(2) copies per reaction. The assay was not influenced by the presence of foreign DNA. This assay was tested against several DNA samples isolated from formation water samples collected from geographically diverse locations of India. The results indicate that this qPCR approach can provide valuable information related to the abundance of the bisulfite reductase gene in harsh environmental samples.

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Section: Introductionmentioning

confidence: 99%

Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR

Agrawal

Lal

2009

FEMS Microbiology Ecology

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“…Fields which depend on accurate knowledge of bacterial transport and biofilm growth in porous media include: pathogen migration to groundwater (Abuashour et al ., 1994); subsurface bioremediation using emplacement or enhancement of indigenous bacteria (Gross and Logan, 1995) and; prediction of the location of populations of microorganisms in oil reservoirs. Biofilm growth can block oil‐bearing rock pores reducing permeability and water and oil flow (MacLeod et al ., 1988; Bass and Lappin‐Scott, 1997) or contaminate crude oil with hydrogen sulphide (Ligthelm et al ., 1991; Sunde et al ., 1993). Often, excessive growth can be removed by reversing the flow of injection water.…”

mentioning

confidence: 99%

A novel approach to investigate biofilm accumulation and bacterial transport in porous matrices

Dunsmore

Bass

Lappin‐Scott

2003

Environmental Microbiology

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Knowledge of bacterial transport through, and biofilm growth in, porous media is vitally important in numerous natural and engineered environments. Despite this, porous media systems are generally oversimplified and the local complexity of cell transport, biofilm formation and the effect of biofilm accumulation on flow patterns is lost. In this study, cells of the sulphate-reducing bacterium, Desulfovibrio sp. EX265, accumulated primarily on the leading faces of obstructions and developed into biofilm, which grew to narrow and block pore throats (at a rate of 12 micro m h(-1) in one instance). This pore blocking corresponded to a decrease in permeability from 9.9 to 4.9 Darcy. Biofilm processes were observed in detail and quantitative data were used to describe the rate of biofilm accumulation temporally and spatially. Accumulation in the inlet zone of the micromodel was 10% higher than in the outlet zone and a mean biofilm height of 28.4 micro m was measured in a micromodel with an average pore height of 34.9 microm. Backflow (flow reversal) of fluid was implemented on micromodels blocked with biofilm growth. Although biofilm surface area cover did immediately decrease (approximately 5%), the biofilm quickly re-established and permeability was not significantly affected (9.4 Darcy). These results demonstrate that the glass micromodel used here is an effective tool for in situ analysis and quantification of bacteria in porous media.

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“…After seawater breakthrough, the well produced a mixture of formation water and seawater together with the oil. The concentration of hydrogen sulfide stayed at a constant low level for at least one pore volume after seawater breakthrough, followed by gradual increase (42). By injection of seawater, the reservoir gradually cools down.…”

Section: Methodsmentioning

confidence: 98%

Distribution of thermophilic marine sulfate reducers in north sea oil field waters and oil reservoirs

Nilsen¹,

Beeder²,

Thorstenson³

et al. 1996

Appl Environ Microbiol

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The distribution of thermophilic marine sulfate reducers in produced oil reservoir waters from the Gullfaks oil field in the Norwegian sector of the North Sea was investigated by using enrichment cultures and genusspecific fluorescent antibodies produced against the genera Archaeoglobus, Desulfotomaculum, and Thermodesulforhabdus. The thermophilic marine sulfate reducers in this environment could mainly be classified as species belonging to the genera Archaeoglobus and Thermodesulforhabdus. In addition, some unidentified sulfate reducers were present. Culturable thermophilic Desulfotomaculum strains were not detected. Specific strains of thermophilic sulfate reducers inhabited different parts of the oil reservoir. No correlation between the duration of seawater injection and the numbers of thermophilic sulfate reducers in the produced waters was observed. Neither was there any correlation between the concentration of hydrogen sulfide and the numbers of thermophilic sulfate reducers. The results indicate that thermophilic and hyperthermophilic sulfate reducers are indigenous to North Sea oil field reservoirs and that they belong to a deep subterranean biosphere.

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Field-Related Mathematical Model to Predict and Reduce Reservoir Souring

Cited by 42 publications

References 6 publications

Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR

Rapid detection and quantification of bisulfite reductase genes in oil field samples using real-time PCR

A novel approach to investigate biofilm accumulation and bacterial transport in porous matrices

Distribution of thermophilic marine sulfate reducers in north sea oil field waters and oil reservoirs

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