Field Testing Rubidium Marking for Quantifying Intercrop Movement of Predatory Arthropods

Prasifka, Jarrad R.; Heinz, Kevin M.; Sansone, Chris

doi:10.1603/0046-225x-30.4.711

Cited by 22 publications

(19 citation statements)

References 27 publications

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“…To avoid cross-contamination, the samples used to detect movement from the thistles to the alfalfa fields were acquired a month after the first Rb application (December 23, 2009). Previous laboratory experimental data have shown that after application of RbCl solution the concentration of rubidium on marked plants and insect decreases quickly with time (Prasifka et al, 2001;Kobelt et al, 2009) and that the concentration of rubidium in the plant tissues is drastically reduced by rain and irrigation (Polavarapu et al, 1992;Long et al, 1998). Therefore, a week prior to the second rubidium application, irrigation was carried out in all fields.…”

Section: Dispersal Of Coccinellids Between Alfalfa and Thistlesmentioning

confidence: 99%

“…Chemical digestion was accomplished via a two-step wet oxidation procedure using concentrated nitric acid and hydrogen peroxide following the method described by Prasifka et al (2001). The digested samples were diluted to 5 ml with ultrapure water, and their rubidium concentrations were estimated using a UNICAM 969 Atomic Absorption Spectrophotometer (AAS).…”

Section: Rubidium Concentration Analysismentioning

confidence: 99%

“…Standard solutions were prepared using distilled water containing rubidium chloride with a purity of 99.8% to obtain a calibration curve. The absorbance of the experimental samples was determined, and the calibration curve was used to calculate their rubidium concentrations as in Prasifka et al (2001). The spectrophotometric determination of the rubidium content of the samples was based on flame AAS using a wavelength of 780 nm.…”

Section: Rubidium Concentration Analysismentioning

confidence: 99%

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Movement between crops and weeds: temporal refuges for aphidophagous insects in Central Chile

et al. 2013

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To determine whether thistles growing at crop edges act as a putative refuge for natural enemies, the seasonal relative abundance of aphidophagous coccinellids was estimated at 0, 10 and 25 m from the edges of three alfalfa fields. Mark-recapture studies were carried out using the trace element rubidium (Rb) to determine whether coccinellids moved between the edges and the alfalfa. The most common aphidophagous coccinellid species were Hippodamia convergens (63%), H. variegata (11%), Rhyzobius lophantae (4%) and Adalia angulifera (4%). In mid-November, the abundance of coccinellids at the edge of (0 m from the edge) the alfalfa plot increased compared to that at the center of the field (25 m from the edge), coinciding with a reduction in the population of aphids at all sampling points. Of the coccinellids captured at the thistle edge, 68% were marked with rubidium, suggesting movement of coccinellids from the alfalfa plot to the thistle growing at its edges. After the thistles were removed, coccinellids returned to the crop, as shown by the presence of marked coccinellids within the alfalfa fields at all three sampling distances. The results of this study suggest that thistles can act as a refuge for coccinellids when aphids are not available in the alfalfa fields.

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Section: Dispersal Of Coccinellids Between Alfalfa and Thistlesmentioning

confidence: 99%

Section: Rubidium Concentration Analysismentioning

confidence: 99%

Section: Rubidium Concentration Analysismentioning

confidence: 99%

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Movement between crops and weeds: temporal refuges for aphidophagous insects in Central Chile

et al. 2013

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“…Graham et al, 1978a, b, Johnson & Reeves, 1995, Prasifka et al, 2001Kobelt et al, 2009) employed rubidium chloride marking of the prey and demonstrated reliable and long detectability (up to 8 days cited by Kobelt et al, 2009) of this metal in subsequently trapped predators, using Inductively Coupled Plasma -Mass Spectrometry (ICP-MS). Unfortunately, the advantages of this method are offset by the complicated machinery required and the very high cost of the ICP-MS analysis (Willbur, 2005).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the diet of a carabid predator using fluorescent marking of prey

Okrouhlík¹,

Foltan²

2015

Eur. J. Entomol.

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Abstract. We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey.

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“…Graham et al 1978a,b;Freeman Long et al 1998). Some work has also been carried out on predatory invertebrates in the field and has shown that Rb is transferred to the predator when it consumes RbCl marked prey (Graham et al 1978a,b;Johnson & Reeves 1995;Prasifka et al 2001). However, neither the effect of time since prey were marked until consumed, nor the amount of time predators stay marked since consuming marked prey has been validated.…”

Section: Introductionmentioning

confidence: 99%

Laboratory validation of rubidium marking of herbivorous insects and their predators

Kobelt¹,

Yen²,

Kitching³

2009

Australian Journal of Entomology

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A simple and effective marking technique is an invaluable tool for tracking the movement of invertebrates in the field. In order to do this, the technique must be validated in a controlled setting. In the first study, two soft bodied herbivores with different feeding strategies and two of their insect predators, also with different feeding strategies, were marked with rubidium (Rb), an elemental marker previously shown to be effective in marking herbivorous invertebrates. The naturally occurring concentration of Rb was determined for both the herbivores and their predators. Rb concentrations were determined in (1) the herbivore species over several days of feeding on rubidium chloride (RbCl) marked plants; (2) in the predatory species immediately after consuming prey marked at different rates with Rb; and (3) in the predatory species for several days after consuming a single meal of Rb marked prey. The concentration of Rb within the marked herbivores was found to be significantly higher than the naturally occurring concentration after eating RbCl marked plants for to up 8 days. A significantly higher concentration of Rb was found in both predators when fed prey that had been on the RbCl sprayed plants for 2 or 8 days compared with naturally occurring concentrations. There was also a significant difference between the Rb concentrations in the controls and each of days 5, 10 and 15 since feeding in both predators. A second smaller study was carried out to examine the effect of feeding Rb marked prey to spiders. The concentration of Rb in the spiders fed marked prey was significantly higher than that of the controls. This suggests that Rb is an effective way of marking both herbivores and their insect and arachnid predators enabling them to be tracked in the field and to determine their movements across habitats.

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Field Testing Rubidium Marking for Quantifying Intercrop Movement of Predatory Arthropods

Cited by 22 publications

References 27 publications

Movement between crops and weeds: temporal refuges for aphidophagous insects in Central Chile

Movement between crops and weeds: temporal refuges for aphidophagous insects in Central Chile

Evaluation of the diet of a carabid predator using fluorescent marking of prey

Laboratory validation of rubidium marking of herbivorous insects and their predators

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