Field Trial of Aqueous and Emulsion Preparations of Entomopathogenic Fungi Against the Asian Citrus Psyllid (Hemiptera: Liviidae) in a Lime Orchard in Mexico

Casique-Valdés, Rebeca; Sánchez-Lara, Braulio M.; Ek-Maas, Julio; Hernández-Guerra, Carlos; Bidochka, Michael; Guízar-Guzmán, Livier; López-Arroyo, J. Isabel; Sánchez-Peña, Sergio R.

doi:10.18474/0749-8004-50.1.79

Cited by 14 publications

(8 citation statements)

References 24 publications

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“…Infection of the red palm weevil , Rhynchophorus ferrugineus Olivier (Coleoptera: Curculionidae) by B. bassiana was determined successful through identification of conidiophores on the insect’s cuticle indicating the completion of the fungus’s life cycle within the insect [11]. Another fungal entomopathogen, Isaria fumosorosea ( Ifr ) Wise (Hypocreales: Cordycipitaceae) has also been demonstrated to infect the Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), the insect vector of citrus greening disease [21,22,23,24,25,26,27,28] and other citrus pests, including the brown citrus aphid, Toxoptera citricida (Kirkaldy) (Hemiptera: Aphididae), glassy-winged sharpshooter, Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), and DRW [29]. …”

Section: Introductionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.

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Section: Introductionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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“…There have been several different methods employed for the use of Cordyceps spp. against AsCP in field conditions, and these include: (1) spraying the target pest on the foliage [ 46 , 47 , 48 , 49 , 50 ]; (2) releasing entomopathogenic fungi using autodisseminators [ 51 , 52 ]; and (3) a combination of these methods [ 37 ]. Each method employed above using fungal formulations containing Cordyceps spp.…”

Section: Discussionmentioning

confidence: 99%

Field Efficacy of Cordyceps javanica, White Oil and Spinetoram for the Management of the Asian Citrus Psyllid, Diaphorina citri

Avery

Duren

Qureshi

et al. 2021

Insects

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Citrus greening disease is devastating the citrus industry in Florida, and the conventional synthetic pesticide applications used to control the vector of the Asian citrus psyllid (AsCP), Diaphorina citri, are rapidly becoming unsustainable. Various laboratory experiments indicate that the entomopathogenic fungus Cordyceps javanica, alone and in combination with horticultural oils, may offer a more sustainable strategy for the management of AsCP. Field studies conducted in 2018 and 2019 in mature citrus indicated that C. javanica alone, C. javanica mixed with white oil, and the chemical standard spinetoram mixed with white oil significantly suppressed AsCP adult populations by 61–83% up to 14 days after treatment in 2018, although colony-forming units of C. javanica were still present on the leaves 21 days after treatment (DAT). Only spinetoram + oil significantly suppressed AsCP, by 100%, up to 7 DAT in 2019. Natural enemies of AsCP, including lady beetles, lacewing larvae and the parasitoid Tamarixia radiata, were observed in the fungal treatments and the untreated control. The AsCP suppression by C. javanica and its compatibility with beneficial organisms suggest the potential use of this entomopathogenic fungus in citrus-integrated pest management.

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“…Liberibacter asiaticus, such threshold is not applicable because studies have shown low transmission associated with a single D. citri adult [11] and even more when nymphal development occurs in healthy plants [9,13,67]. Furthermore, there are diverse natural enemies contributing to the reduction in the vector population [68][69][70][71] that would potentially and naturally assist the prevention of the vector from reaching efficient transmission levels. Hence, it is feasible to consider the use of SPRT sequential plans to support decision making in vector control.…”

Section: Discussionmentioning

confidence: 99%

“…This avoids intensive and extensive chemical control of the vector such as the program that exists in Brazil [22]. Furthermore, it would increase the opportunity for biological control [68][69][70][71]. In this manner, the current production systems could move towards the sustainable citrus industry of the future.…”

Section: Discussionmentioning

confidence: 99%

Spatial Distribution and Development of Sequential Sampling Plans for Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Díaz-Padilla

López-Arroyo²,

Guajardo-Panes

et al. 2021

Agronomy

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Vector control in huanglongbing management has been conducted on a calendar basis resulting in high production costs. We addressed this issue and proposed a sequential sampling plan to support decision making for intervention against Diaphorina citri Kuwayama, which is involved in the transmission of the bacteria Candidatus Liberibacter asiaticus, associated with such lethal disease. We analyzed 3,264,660 records from samples gathered from the Mexican trapping program for the monitoring of D. citri; it included weekly inspection of 86,004 yellow sticky traps distributed in the country. Spatial distribution of the insect, estimation of a common k (kc), and sequential sampling plans based on Sequential Probability Ratio Test (SPRT) were determined. Taylor’s power law coefficients were ≥1 indicating aggregation in the spatial distribution of the insect. Common k ranged from 0.0183 to 0.2253 and varied independently of geographic zone or citrus species. We obtained 18 sequential sampling plans, one for each state. In the Average Sample Number (ASN) function, the minimal number of samples to make a decision ranged from 17 to 65. In the Operational Characteristic (OC) function, probabilities for a correct intervention at the threshold of 0.2 D. citri adults/trap in most cases were above 80%. In a field evaluation, the application of sampling plans yielded savings obtained by reduction in the number of interventions for insect control.

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Field Trial of Aqueous and Emulsion Preparations of Entomopathogenic Fungi Against the Asian Citrus Psyllid (Hemiptera: Liviidae) in a Lime Orchard in Mexico

Cited by 14 publications

References 24 publications

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Field Efficacy of Cordyceps javanica, White Oil and Spinetoram for the Management of the Asian Citrus Psyllid, Diaphorina citri

Spatial Distribution and Development of Sequential Sampling Plans for Diaphorina citri Kuwayama (Hemiptera: Liviidae)

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