Fifteen-Piconewton Force Detection from Neural Growth Cones Using Nanowire Arrays

Hällström, Waldemar; Lexholm, Monica; Suyatin, Dmitry; Hammarin, Greger; Hessman, Dan; Samuelson, Lars; Montelius, Lars; Kanje, Martin; Prinz, Christelle

doi:10.1021/nl902675h

Cited by 114 publications

(141 citation statements)

References 24 publications

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“…These changes in modes of cell adhesions could have implications for applications within cell migration 15 , cell force studies 14 , as well as cell capture 58 and stem cell guidance 27 .…”

Section: Cell Interactions With Su-8 Nanostructuresmentioning

confidence: 99%

Tunable high aspect ratio polymer nanostructures for cell interfaces

et al. 2015

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Nanoscale topographies and chemical patterns can be used as synthetic cell interfaces with a range of applications including study and control of cellular processes. Herein, we describe the fabrication of high aspect ratio nanostructures using electron beam lithography in the epoxy-based polymer SU-8. We show how nanostructure geometry, position and fluorescent properties can be tuned, allowing flexible device design. Further, thiol-epoxide reactions were developed to give effective and specific modification of SU-8 surface chemistry. SU-8 nanostructures were made directly on glass cover slips, enabling the use of high resolution optical techniques such as live-cell confocal, total internal reflection and 3D structured illumination microscopy to investigate cell interactions with the nanostructures. Details of cell adherence and spreading, plasma membrane conformation and actin organization in response to high aspect ratio nanopillars and nanolines were investigated. The versatile structural and chemical properties combined with high resolution cell imaging capabilities of this system are an important step towards better understanding and controlling cell interactions with nanomaterials.

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“…These changes in modes of cell adhesions could have implications for applications within cell migration 15 , cell force studies 14 , as well as cell capture 58 and stem cell guidance 27 .…”

Section: Cell Interactions With Su-8 Nanostructuresmentioning

confidence: 99%

Tunable high aspect ratio polymer nanostructures for cell interfaces

et al. 2015

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“…The results in Figure 3(b) and (d) indicate that the inclined DFOTs can provide forces ranging from sub-pN to tens of pN. It implies that the inclined DFOTs can be used to measure cellular forces, since various cellular forces lie in this range, such as those generated by neural growth cone [27] and by a single actin filament [28]. Compared with the force sensing range of AFM, which is in the range of 10 pN to 100 nN [29], the force range of inclined DFOTs may extend the range of the biological applications and measurable quantities that may be challenging for AFM.…”

Section: Optical Trapping Spring Constant Calibration In Watermentioning

confidence: 90%

Fiber Optical Tweezers for Applying and Measuring Forces in a 3D Solid Compartment

Ti¹,

Thanh²,

Shen³

et al. 2018

Selected Topics on Optical Fiber Technologies and Applications

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“…Of interest, the unique properties of each nanowire allow additional cellular applications. For example, dissociated sensory neurons cultured on vertically-grown epitaxial gallium phosphide nanowires (GaP NWs) showed a higher viability than planar gallium phosphide substrates ( Figure 5 (C)) [58]. Interestingly, in other work, GaP NWs were utilized to measure cellular forces by analyzing the bending of GaP NWs (Figure 5 (D)) [59].…”

Section: Other Inorganic Nanowiresmentioning

confidence: 99%

Synthetic nanowire/nanotube-based solid substrates for controlled cell growth

Baik

Namgung

et al. 2014

Nano Convergence

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The behaviour of cells can be controlled by various microenvironments such as nanostructured cell-culture substrates with controlled nanotopography and chemical properties. One of promising substrates for controlled cell growth is a solid substrate comprised of synthetic one-dimensional nanostructures such as polymer nanofibers, carbon-based nanotubes/nanofibers, and inorganic nanowires. Such nanotube/nanowire structures have a similar dimension as extracellular matrix fibers, and their nanotopography and chemical properties can be easily controlled, which expands their possible applications in controlling the growth and differentiation of cells. This paper provides a concise review on the recent applications of solid substrates based on synthetic nanowires/nanotubes for controlled cell growth and differentiation.

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Fifteen-Piconewton Force Detection from Neural Growth Cones Using Nanowire Arrays

Cited by 114 publications

References 24 publications

Tunable high aspect ratio polymer nanostructures for cell interfaces

Tunable high aspect ratio polymer nanostructures for cell interfaces

Fiber Optical Tweezers for Applying and Measuring Forces in a 3D Solid Compartment

Synthetic nanowire/nanotube-based solid substrates for controlled cell growth

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