Fifty years of research on serotypes and mating types in Dileptus anser: A review

Uspenskaya, Z. I.; Yudin, A. L.

doi:10.1016/j.ejop.2015.12.004

Cited by 5 publications

(4 citation statements)

References 32 publications

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“…Differently from Blepharisma pheromones, those isolated from Dileptus anser and various species of Euplotes, namely E. raikovi, E. octocarinatus, E. nobilii, E. petzi and E. crassus, all are high-stable proteins that maintain longlasting integrity and biological activity in their natural environment. For the D. anser pheromones (three isolated from interbreeding cultures), the characterization is narrowed to a molecular mass in the range of 3-4.5 kDa and a strong propensity to associate into unstable oligomers (Parfenova et al, 1989;Uspenskaya & Yudin, 2016). Far more characterized are Euplotes pheromones, known to be encoded by multiple series of codominant alleles at the mat-locus of the micronuclear genome (Vallesi et al, 2014).…”

Section: Wat E R-bor N E Ph E Romon E Smentioning

confidence: 99%

“…For the D . anser pheromones (three isolated from interbreeding cultures), the characterization is narrowed to a molecular mass in the range of 3–4.5 kDa and a strong propensity to associate into unstable oligomers (Parfenova et al, 1989; Uspenskaya & Yudin, 2016). Far more characterized are Euplotes pheromones, known to be encoded by multiple series of codominant alleles at the mat ‐locus of the micronuclear genome (Vallesi et al, 2014).…”

Section: Water‐borne Pheromonesmentioning

confidence: 99%

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Bioactive molecules from ciliates: Structure, activity, and applicative potential

Alimenti

Buonanno

Giuseppe

et al. 2022

J Eukaryotic Microbiology

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BIOACTIVE molecules are generally recognize as those natural products-organic compounds that are synthesized by living systems-which common wisdom suggests to be structurally 'optimized' and selected for by evolution in order to confer their source organisms with a competitive, ecological or evolutionary advantage (Hanson, 2003). Bioactive molecules are commonly regarded as playing no direct role in the essential processes of the life. This role is distinctive of primary metabolites (Thirumurugan et al., 2018). Nevertheless, they govern and shape the reproductive success of their source organisms by driving defense/offence mechanisms, interactions with the environment and phenomena of self/nonself discrimination. This wide range of activities overall account well for the strong interest that bioactive molecules arouse both in basic research and bioprospecting for drug discovery and design in the fields of medical chemistry and pharmacognosy (Khazir et al., 2014;Newman & Cragg, 2014).The vast array of effective sources of bioactive molecules is historically dominated by species from prokaryotes

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Section: Wat E R-bor N E Ph E Romon E Smentioning

confidence: 99%

Section: Water‐borne Pheromonesmentioning

confidence: 99%

Bioactive molecules from ciliates: Structure, activity, and applicative potential

Alimenti

Buonanno

Giuseppe

et al. 2022

J Eukaryotic Microbiology

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“…However, a full appreciation of this identification has suffered from descriptions mostly reported in Russian journals that are not readily available abroad. In retrieving 50 years of research on D. anser at the Institute of Cytology in St. Petersburg, Uspenskaya and Yudin (2016) have now meritoriously made more accessible their story. Pheromone secretion in D.…”

Section: Dileptus Pheromonesmentioning

confidence: 99%

Revisiting fifty years of research on pheromone signaling in ciliates

Luporini

Pedrini

Alimenti

et al. 2016

European Journal of Protistology

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Among protists, pheromones have been identified in a great variety of algal species for their activity in driving gamete-gamete interactions for fertilization. Analogously in ciliates, pheromones have been identified for their activity in inducing the sexual phenomenon of conjugation. Although this identification was pioneered by Kimball more than fifty years ago, an effective isolation and chemical characterization of ciliate pheromones has remained confined to species of Blepharisma, Dileptus and Euplotes. In Euplotes species, in which the molecular structures have been determined, pheromones form species-specific families of structurally homologous helical, cysteine-rich, highly-stable proteins. Being structurally homologous, they can bind cells in competition with one another, raising interesting functional analogies with the families of growth factors and cytokines that regulate cell differentiation and development in higher organisms. In addition to inducing conjugation by binding cells in heterologous fashion, Euplotes pheromones act also as autocrine growth factors by binding to, and promoting the vegetative reproduction of the same cells from which they originate. This autocrine activity is most likely primary, providing a concrete example of how the original function of a molecule can be obscured during evolution by the acquisition of a new one.

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“…As is common among spirotrichous ciliates (Dini and Nyberg ), Euplotes controls the specificity of its social interactions at a single mat locus of the transcriptionally silent germ‐line micronucleus, and expresses the multiple alleles of this locus in the transcriptionally active somatic macronucleus with the synthesis of the “mating‐type factors” that are responsible for the chemical specificity to each cell (mating) type. As is the case in other ciliates, Blepharisma (Miyake ) and Dileptus (Uspenskaya and Yudin ) in particular, these factors are constitutively secreted by Euplotes as water‐borne signaling pheromones into the extracellular environment (Miceli et al 1983), which has greatly facilitated their isolation and chemical analysis. A structural characterization of Euplotes pheromones has so far been achieved, along with a characterization of the macronuclear pheromone coding genes, in Euplotes raikovi , Euplotes octocarinatus , Euplotes nobilii , and Euplotes crassus (Luporini et al.…”

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confidence: 99%

Molecular Structures and Coding Genes of the Water‐Borne Protein Pheromones of Euplotes petzi, an Early Diverging Polar Species of Euplotes

Pedrini

Suter-Stahel²,

Vallesi

et al. 2016

J Eukaryotic Microbiology

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Euplotes is diversified into dozens of widely distributed species that produce structurally homologous families of water-borne protein pheromones governing self-/nonself-recognition phenomena. Structures of pheromones and pheromone coding genes have so far been studied from species lying in different positions of the Euplotes phylogenetic tree. We have now cloned the coding genes and determined the NMR molecular structure of four pheromones isolated from Euplotes petzi, a polar species which is phylogenetically distant from previously studied species and forms the deepest branching clade in the tree. The E. petzi pheromone genes have significantly shorter sequences than in other congeners, lack introns, and encode products of only 32 amino acids. Likewise, the three-dimensional structure of the E. petzi pheromones is markedly simpler than the three-helix up-down-up architecture previously determined in another polar species, Euplotes nobilii, and in a temperate-water species, Euplotes raikovi. Although sharing the same up-down-up architecture, it includes only two short α-helices that find their topological counterparts with the second and third helices of the E. raikovi and E. nobilii pheromones. The overall picture that emerges is that the evolution of Euplotes pheromones involves progressive increases in the gene sequence length and in the complexity of the three-dimensional molecular structure.

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Fifty years of research on serotypes and mating types in Dileptus anser: A review

Cited by 5 publications

References 32 publications

Bioactive molecules from ciliates: Structure, activity, and applicative potential

Bioactive molecules from ciliates: Structure, activity, and applicative potential

Revisiting fifty years of research on pheromone signaling in ciliates

Molecular Structures and Coding Genes of the Water‐Borne Protein Pheromones of Euplotes petzi, an Early Diverging Polar Species of Euplotes

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