Fighting Acinetobacter baumannii infections with the acylase PvdQ

Vogel, Jan; Jansen, Lisa; Setroikromo, Rita; Cavallo, Francis M.; Dijl, Jan Maarten van; Quax, Wim J.

doi:10.1016/j.micinf.2022.104951

Cited by 4 publications

(6 citation statements)

References 34 publications

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“…In this report, 2,4-DBP has acted synergistically with the conventional antibiotics tested against A. baumannii . Obtained results are in accordance with several other research groups that worked on secondary metabolites against A. baumannii [53, 54]. Our findings strengthen the combinatorial use of anti-biofilm and other anti-microbial agents to combat the bacterial pathogenesis.…”

Section: Discussionsupporting

confidence: 91%

“…The elimination of A. baumannii biofilms from living and non-living subjects is a huge challenge for clinicians leading to a high demand of novel therapeutics. Vogel et al [53] concluded that PvdQ, an acylase which targets and chelates lactone ring, was effective in reducing the AHL-dependent biofilm production in A. baumannii. On the other hand, when compared to the previous report by Sivaranjani et al [54] and Chang et al [55], where alpha-mangostin and EDTA was only able to stop the early biofilm formation, our study showed inhibition of both early as well as preformed biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…Vogel et al . [53] concluded that PvdQ, an acylase which targets and chelates lactone ring, was effective in reducing the AHL-dependent biofilm production in A. baumannii . On the other hand, when compared to the previous report by Sivaranjani et al .…”

Section: Discussionmentioning

confidence: 99%

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In vitro and in silico assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against Acinetobacter baumannii

Jha,

Gopu,

Sivasankar

et al. 2024

Journal of Medical Microbiology

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Introduction. Acinetobacter baumannii is a nosocomial pathogen with a high potential to cause food-borne infections. It is designated as a critical pathogen by the World Health Organization due to its multi-drug resistance and mortalities reported. Biofilm governs major virulence factors, which promotes drug resistance in A. baumannii. Thus, a compound with minimum selection pressure on the pathogen can be helpful to breach biofilm-related virulence. Hypothesis/Gap Statement. To identify anti-biofilm and anti-virulent metabolites from extracts of wild Mangifera indica (mango) brine pickle bacteria that diminishes pathogenesis and resistance of A. baumannii. Aim. This study reports anti-biofilm and anti-quorum sensing (QS) efficacy of secondary metabolites from bacterial isolates of fermented food origin. Method. Cell-free supernatants (CFS) of 13 bacterial isolates from fermented mango brine pickles were screened for their efficiency in inhibiting biofilm formation and GC-MS was used to identify its metabolites. Anti-biofilm metabolite was tested on early and mature biofilms, pellicle formation, extra polymeric substances (EPS), cellular adherence, motility and resistance of A. baumannii. Gene expression and in silico studies were also carried out to validate the compounds efficacy. Results. CFS of TMP6b identified as Bacillus vallismortis, inhibited biofilm production (83.02 %). Of these, major compound was identified as 2,4-Di-tert-butyl phenol (2,4-DBP). At sub-lethal concentrations, 2,4-DBP disrupted both early and mature biofilm formation. Treatment with 2,4-DBP destructed in situ biofilm formed on glass and plastic. In addition, key virulence traits like pellicle (77.5 %), surfactant (95.3 %), EPS production (3-fold) and cell adherence (65.55 %) reduced significantly. A. baumannii cells treated with 2,4-DBP showed enhanced sensitivity towards antibiotics, oxide radicals and blood cells. Expression of biofilm-concomitant virulence genes like csuA/B, pgaC, pgaA, bap, bfmR, katE and ompA along with QS genes abaI, abaR significantly decreased. The in silico studies further validated the higher binding affinity of 2,4-DBP to the AbaR protein than the cognate ligand molecule. Conclusion. To our knowledge, this is the first report to demonstrate 2,4- DBP has anti-pathogenic potential alone and with antibiotics by in vitro, and in silico studies against A. baumannii. It also indicates its potential use in therapeutics and bio-preservatives.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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In vitro and in silico assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against Acinetobacter baumannii

Jha,

Gopu,

Sivasankar

et al. 2024

Journal of Medical Microbiology

View full text Add to dashboard Cite

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“…The inhibition of quorum-sensing, i.e., quorum-quenching, is one of the promising strategies to impair biofilm formation. For instance, the P. aeruginosa acylase PvdQ is able to cleave the acyl homoserine lactones of A. baumannii which are the mediators of quorum-sensing in this pathogen, affecting bacterial communication and, as a consequence, impairing biofilm formation in vitro ( Vogel et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Recent advances to combat ESKAPE pathogens with special reference to essential oils

et al. 2022

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Biofilm-associated bacteria, especially ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), are a serious challenge worldwide. Due to the lack of discovery of novel antibiotics, in the past two decades, it has become necessary to search for new antibiotics or to study synergy with the existing antibiotics so as to counter life-threatening infections. Nature-derived compounds/based products are more efficient than the chemically synthesized ones with less resistance and lower side effects. In this descriptive review, we discuss the most promising therapeutics for the treatment of ESKAPE-related biofilms. The first aspect includes different types of natural agents [botanical drugs, essential oils (EOs), antimicrobial peptides, bacteriophages, and endolysins] effective against ESKAPE pathogens. The second part of the review deals with special references to EOs/essential oil components (EOCs) (with some exclusive examples), mode of action (via interfering in the quorum-sensing pathways, disruption of biofilm and their inhibitory concentrations, expression of genes that are involved, other virulence factors), existing in literature so far. Moreover, different essential oils and their major constituents were critically discussed using in vivo models to target ESKAPE pathogens along with the studies involving existing antibiotics.

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“…Besides, total extracts of Tinospora cordifolia, flavonoids of Glycyrrhiza glabra (Bhargava et al, 2015), essential oils from Eucalyptus (Luís et al, 2016), and linalool from Coriandrum sativum (Alves et al, 2016) inhibited QS and biofilm formation in A. baumannii. Furthermore, disruption of AHL signals by lactonases AiiA (Chow et al, 2014), α/ß hydrolase AidA (Lopez et al, 2017) and PvdQ acylase enzyme (Vogel et al, 2022), MomL of Muricauda olearia significantly eliminates biofilm formation from A. baumannii and prevents bacterial motility (Zhang et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

El-Shaer

Shaldam

Shaaban

2022

Journal of Applied Microbiology

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Aim Quorum sensing (QS) inhibition is a promising strategy to suppress bacterial virulence and control infection caused by Gram‐negative and Gram‐positive bacteria. This study explores the QS inhibiting activity of the non‐steroidal anti‐inflammatory drugs (NSAIDs) in Acinetobacter baumannii. Methods and Results Ketoprofen, piroxicam and indomethacin revealed QS inhibition via elimination of violacein production of the reporter strain Chromobacterium violaceum ATCC 12472 without affecting bacterial growth. The minimal inhibitory concentration (MIC) of ketoprofen, piroxicam and indomethacin was determined against A. baumannii strains ATCC 17978, ATCC 19606, A1, A11 and A27 by the microbroth dilution method. The MICs of ketoprofen against tested isolates were 0.7–6.25 mg ml−1, piroxicam MICs were 1.25–2.5 mg ml−1, and indomethacin MICs were 3.12–12.5 mg ml−1. Those compounds significantly inhibited QS‐associated virulence factors such as biofilm formation, and surface motility, as well as, significantly increased bacterial tolerance to oxidative stress without affecting bacterial growth. On the molecular level, the three compounds significantly inhibited the transcription of QS regulatory genes abaI/abaR and biofilm‐regulated genes cusD and pgaB. Molecular docking analysis revealed the potent binding affinity of the three compounds with AbaI via hydrogen and/or hydrophobic bonds. Conclusion These results indicate that NSAIDs, ketoprofen, piroxicam and indomethacin, could be potential inhibitors of the QS and could suppress the QS‐related virulence factors of A. baumannii. Significance and Impact Ketoprofen, piroxicam and indomethacin could provide promising implications and strategies for combating the virulence and pathogenesis of A. baumannii.

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Fighting Acinetobacter baumannii infections with the acylase PvdQ

Cited by 4 publications

References 34 publications

In vitro and in silico assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against Acinetobacter baumannii

In vitro and in silico assessment of anti-biofilm and anti-quorum sensing properties of 2,4-Di-tert butylphenol against Acinetobacter baumannii

Recent advances to combat ESKAPE pathogens with special reference to essential oils

Ketoprofen, piroxicam and indomethacin-suppressed quorum sensing and virulence factors in Acinetobacter baumannii

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