Fighting Methicillin-Resistant Staphylococcus aureus with Targeted Nanoparticles

Andrade, Stéphanie; Ramalho, Maria João; Santos, Sílvio Roberto Branco; Melo, Luís D. R.; Santos, Rita S.; Guimarães, Nuno; Azevedo, Nuno F.; Loureiro, Joana A.; Pereira, Maria do Carmo

doi:10.3390/ijms24109030

Cited by 9 publications

(3 citation statements)

References 80 publications

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“…It is responsible for endocarditis, chronic osteomyelitis, pneumonia and osteoarthritis. Among the main mechanisms of antibiotic resistance, in the case of members of this species, we note the change in the consistency of the cell wall, the presence of a greater number of efflux pumps in the external cellular structures and biofilm formation [ 48 , 49 ]. In this context, highlighting a possible sensitivity to the plant extract MOE may prove to be valuable for the development of alternative strategies to combat this pathogen both in the case of systemic and local infections.…”

Section: Resultsmentioning

confidence: 99%

The Antimicrobial Potency of Mesoporous Silica Nanoparticles Loaded with Melissa officinalis Extract

Petrișor,

Motelica,

Trușcǎ

et al. 2024

Pharmaceutics

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Melissa officinalis is an important medicinal plant that is used and studied intensively due to its numerous pharmacological effects. This plant has numerous active compounds with biomedical potential; some are volatile, while others are sensitive to heat or oxygen. Therefore, to increase stability and prolong biological activities, the natural extract can be loaded into various nanostructured systems. In this study, different loading systems were obtained from mesoporous silica, like Mobile Composition of Matter family (MCM) with a hexagonal (MCM-41) or cubic (MCM-48) pore structure, simple or functionalized with amino groups (using 3-aminopropyl) such as triethoxysilane (APTES). Thus, the four materials were characterized from morphological and structural points of view by scanning electron microscopy, a BET analysis with adsorption–desorption isotherms, Fourier-transform infrared spectroscopy (FTIR) and a thermogravimetric analysis coupled with differential scanning calorimetry. Natural extract from Melissa officinalis was concentrated and analyzed by High-Performance Liquid Chromatography to identify the polyphenolic compounds. The obtained materials were tested against Gram-negative bacteria and yeasts and against both reference strains and clinical strains belonging to Gram-positive bacteria that were previously isolated from intra-hospital infections. The highest antimicrobial efficiency was found against Gram-positive and fungal strains. Good activity was also recorded against methicillin-resistant S. aureus, the Melissa officinalis extract inhibiting the production of various virulence factors.

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Section: Resultsmentioning

confidence: 99%

The Antimicrobial Potency of Mesoporous Silica Nanoparticles Loaded with Melissa officinalis Extract

Petrișor,

Motelica,

Trușcǎ

et al. 2024

Pharmaceutics

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“…One such strategy currently being explored is the delivery of antibiotics or antimicrobials in general via nanomedicines [ 19 , 20 , 21 ]. For example, it has been reported that Dapto encapsulated in polymeric nanoparticles demonstrated higher activity towards established Staphylococci biofilms compared to the free drug [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

Gkartziou,

Plota,

Kypraiou

et al. 2024

Pharmaceutics

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The purpose of the present study was to investigate the anti-staphylococcal activity of liposomal daptomycin against four biofilm-producing S. aureus and S. epidermidis clinical strains, three of which are methicillin-resistant. Neutral and negatively charged daptomycin-loaded liposomes were prepared using three methods, namely, thin-film hydration (TFH), a dehydration–rehydration vesicle (DRV) method, and microfluidic mixing (MM); moreover, they were characterized for drug encapsulation (EE%), size distribution, zeta-potential, vesicle stability, drug release, and drug integrity. Interestingly, whilst drug loading in THF and DRV nanosized (by extrusion) vesicles was around 30–35, very low loading (~4%) was possible in MM vesicles, requiring further explanatory investigations. Liposomal encapsulation protected daptomycin from degradation and preserved its bioactivity. Biofilm mass (crystal violet, CV), biofilm viability (MTT), and growth curve (GC) assays evaluated the antimicrobial activity of neutral and negatively charged daptomycin-liposomes towards planktonic bacteria and biofilms. Neutral liposomes exhibited dramatically enhanced inhibition of bacterial growth (compared to the free drug) for all species studied, while negatively charged liposomes were totally inactive. Biofilm prevention and treatment studies revealed high antibiofilm activity of liposomal daptomycin. Neutral liposomes were more active for prevention and negative charge ones for treating established biofilms. Planktonic bacteria as well as the matured biofilms of low daptomycin-susceptible, methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus epidermidis (MRSE) strains were almost completely eradicated by liposomal-daptomycin, indicating the need for their further exploration as antimicrobial therapeutics.

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“…[13,14] Additionally, Methicillin-Resistant Staphylococcus aureus (MRSA) exhibits global dissemination, associated with approximately 90 % of cases of infections acquired in both hospital and community settings. [15] Therefore, this research aims to evaluate the biological activities (antioxidant, antibacterial, and anti-biofilm formation) of geopropolis from Melipona scutellaris (uruçu bee) against S. aureus isolates with the purpose of contributing to a natural therapeutic alternative for combating infections caused by this pathogen.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Biological Activities of Geopopolis Extract from the Stingless bee (Melipona scutellaris) in Isolates of Staphylococcus aureus

Albuquerque Borges,

de Oliveira e Lima,

Veras

et al. 2024

Chemistry & Biodiversity

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This work aims to evaluate the biological activities of the EEGP, in order to contribute with a natural therapeutic alternative, to face infections. The EEGP MIC tests showed antibacterial activity against two strains of S. aureus (LPM 63 and LPM 86), both at concentrations of 550 µg/mL. The MBC performed with the inhibition values showed that the EEGP has bacteriostatic activity in both strains. Biofilm inhibition rates exhibited an average value greater than 65% at the highest concentration. The EEGP antioxidant potential test showed good antioxidant activity (IC50) of 11.05 ± 1.55 µg/mL. In the cytotoxicity test against HaCat cells, after 24 hours, EEGP induced cell viability at the three tested concentrations (550 µg/mL: 81.68 ± 3.79%; 1100 µg/mL: 67.10 ± 3.76 % ;2200 µg/mL: 67.40 ± 1.86%). In view of the above, the safe use of EEGP from the brazilian northeast could be proven by the cytotoxicity test, and its use as an antioxidant and antibacterial agent has proven to be effective, as an alternative in combating oxidative stress and microorganisms such as S. aureus, which, through the spread and ongoing evolution of drug resistance, generates an active search for effective solutions.

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Fighting Methicillin-Resistant Staphylococcus aureus with Targeted Nanoparticles

Cited by 9 publications

References 80 publications

The Antimicrobial Potency of Mesoporous Silica Nanoparticles Loaded with Melissa officinalis Extract

The Antimicrobial Potency of Mesoporous Silica Nanoparticles Loaded with Melissa officinalis Extract

Daptomycin Liposomes Exhibit Enhanced Activity against Staphylococci Biofilms Compared to Free Drug

Cytotoxicity and Biological Activities of Geopopolis Extract from the Stingless bee (Melipona scutellaris) in Isolates of Staphylococcus aureus

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