Fighting Mixed-Species Microbial Biofilms With Cold Atmospheric Plasma

Rao, Yifan; Shang, Wenjun; Yang, Yi; Zhou, Ren; Rao, Xiancai

doi:10.3389/fmicb.2020.01000

Cited by 52 publications

(43 citation statements)

References 87 publications

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“…Due to their multi-target mode of action and large quantities of ROS production which overwhelm the antioxidant defenses in pathogens, the additional advantage of these new techniques is the lack of development of resistance mechanisms ( Tavares et al, 2010 ; Kvam et al, 2012 ). As novel ROS-inducing strategies, aPDT and CAP showed excellent potential to tackle difficult-to-eradicate infections as alternative treatments in wound healing, dental cure, and food decontamination ( Wilson and Patterson, 2008 ; Rao et al, 2020 ).…”

Section: Novel Antimicrobial Strategies Centered Around Rosmentioning

confidence: 99%

Reactive Oxygen Species in Pathogen Clearance: The Killing Mechanisms, the Adaption Response, and the Side Effects

et al. 2021

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Reactive oxygen species (ROS) are attractive weapons in both antibiotic-mediated killing and host-mediated killing. However, the involvement of ROS in antibiotic-mediated killing and complexities in host environments challenge the paradigm. In the case of bacterial pathogens, the examples of some certain pathogens thriving under ROS conditions prompt us to focus on the adaption mechanism that pathogens evolve to cope with ROS. Based on these, we here summarized the mechanisms of ROS-mediated killing of either antibiotics or the host, the examples of bacterial adaption that successful pathogens evolved to defend or thrive under ROS conditions, and the potential side effects of ROS in pathogen clearance. A brief section for new antibacterial strategies centered around ROS was also addressed.

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Section: Novel Antimicrobial Strategies Centered Around Rosmentioning

confidence: 99%

Reactive Oxygen Species in Pathogen Clearance: The Killing Mechanisms, the Adaption Response, and the Side Effects

et al. 2021

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“…Therefore, they organize themselves in a symbiotic community supporting each other [ 5 ]. Multi-species biofilms are less susceptible to chemical antimicrobial agents, antibiotics [ 84 ], and to CAP [ 85 ]. In the study of Koban et al, the RF for S. mutans biofilm was 3.1, whereas the RF for an ex-vivo, saliva biofilm was 1.6 log 10 CFU using an argon plasma jet for 60 s [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

et al. 2021

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Interest in the application of cold atmospheric plasma (CAP) in the medical field has been increasing. Indications in dentistry are surface modifications and antimicrobial interventions. The antimicrobial effect of CAP is mainly attributed to the generation of reactive oxygen and reactive nitrogen species. The aim of this article is to systematically review the available evidence from in-vitro studies on the antimicrobial effect of CAP on dental pathogens. A database search was performed (PubMed, Embase, Scopus). Data concerning the device parameters, experimental set-ups and microbial cultivation were extracted. The quality of the studies was evaluated using a newly designed assessment tool. 55 studies were included (quality score 31–92%). The reduction factors varied strongly among the publications although clusters could be identified between groups of set pathogen, working gases, and treatment time intervals. A time-dependent increase of the antimicrobial effect was observed throughout the studies. CAP may be a promising alternative for antimicrobial treatment in a clinically feasible application time. The introduced standardized protocol is able to compare the outcome and quality of in-vitro studies. Further studies, including multi-species biofilm models, are needed to specify the application parameters of CAP before CAP should be tested in randomized clinical trials.

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“…Importantly, due to cooperative interactions between them, the multispecies biofilms may display greater resistance to external stressful factors, including antibiotics and disinfectants, relative to the single-species community. This property is probably related to increased biomass and/or an altered composition of the EPS matrix [ 51 , 52 , 53 ].…”

Section: Characterization Of Bacterial Biofilmsmentioning

confidence: 99%

Bacteriophage-Derived Depolymerases against Bacterial Biofilm

et al. 2021

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In addition to specific antibiotic resistance, the formation of bacterial biofilm causes another level of complications in attempts to eradicate pathogenic or harmful bacteria, including difficult penetration of drugs through biofilm structures to bacterial cells, impairment of immunological response of the host, and accumulation of various bioactive compounds (enzymes and others) affecting host physiology and changing local pH values, which further influence various biological functions. In this review article, we provide an overview on the formation of bacterial biofilm and its properties, and then we focus on the possible use of phage-derived depolymerases to combat bacterial cells included in this complex structure. On the basis of the literature review, we conclude that, although these bacteriophage-encoded enzymes may be effective in destroying specific compounds involved in the formation of biofilm, they are rarely sufficient to eradicate all bacterial cells. Nevertheless, a combined therapy, employing depolymerases together with antibiotics and/or other antibacterial agents or factors, may provide an effective approach to treat infections caused by bacteria able to form biofilms.

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Fighting Mixed-Species Microbial Biofilms With Cold Atmospheric Plasma

Cited by 52 publications

References 87 publications

Reactive Oxygen Species in Pathogen Clearance: The Killing Mechanisms, the Adaption Response, and the Side Effects

Reactive Oxygen Species in Pathogen Clearance: The Killing Mechanisms, the Adaption Response, and the Side Effects

The Antimicrobial Effect of Cold Atmospheric Plasma against Dental Pathogens—A Systematic Review of In-Vitro Studies

Bacteriophage-Derived Depolymerases against Bacterial Biofilm

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