“…Morphologically, T. ingrica was defined as a Thioploca species having a trichome 2.0-4.5 mm in diameter (Wislouch, 1912;Maier, 1984). Organisms that fit this description have been found in freshwater and brackish sediments of various localities, and the placement of these organisms in the same species has been generally supported by their 16S rRNA gene sequences (Maier and Murray, 1965;Nishino et al, 1998;Zemskaya et al, 2001Zemskaya et al, , 2009Dermott and Legner, 2002;Kojima et al, 2003Kojima et al, , 2006Høgslund et al, 2010;Nemoto et al, 2011Nemoto et al, , 2012Salman et al, 2011). Nitrate accumulation by T. ingrica was reported in previous studies, although the intracellular concentrations were much lower than in relatives with large vacuoles (Zemskaya et al, 2001;Kojima et al, 2007;Høgslund et al, 2010).…”