Filamin Interacts with Epithelial Sodium Channel and Inhibits Its Channel Function

Wang, Qian; Dai, Xiao-Qing; Li, Qiang; Tuli, Jagdeep; Liang, Gengqing; Li, Shayla S.; Chen, Xing-Zhen

doi:10.1074/jbc.m112.396408

Cited by 22 publications

(12 citation statements)

References 36 publications

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“…Biotinylation-Biotinylation and Western blot analyses were carried out to measure the protein expression level of each isoform based upon a previously published protocol (19) with modification. Oocytes were washed three times with phosphate buffer saline (PBS), pH 8.0; then they were incubated in 2 mM Sulfo-NHS-LC-Biotin (Pierce) at room temperature for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Critical Roles of Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) in Substrate Selectivity and Urate Transport

Long

Panwar

Witkowska

et al. 2015

Journal of Biological Chemistry

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Background: A hydrophobic residue in TM7 of the hSLC2A9 was found to affect hexose transport. Results: Both Ile-335 and Trp-110 affect fructose trans-acceleration of urate, whereas only Trp-110 directly affects urate transport. Conclusion: hSLC2A9 handles urate/fructose transport differently. Significance: This study provides further information of how hSLC2A9 handling its substrates could be beneficial for future pharmaceutical treatments used in related diseases.

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Section: Methodsmentioning

confidence: 99%

Critical Roles of Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) in Substrate Selectivity and Urate Transport

Long

Panwar

Witkowska

et al. 2015

Journal of Biological Chemistry

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“…Future studies should be performed to investigate whether Per1 indirectly regulates ENaC protein expression and activity at the apical plasma membrane by regulating chaperone proteins involved in its insertion into the membrane and effector proteins involved in its degradation and recycling back to the membrane. Moreover, studies should be performed to determine whether Per1 regulates actin cytoskeleton proteins such as myristoylated alaninerich protein kinase C substrate and filamin, which have been shown to play an essential role in stabilizing ENaC at the membrane (1,2,19,31).…”

Section: Direct Inhibition Of Per1 Reduces Enac Activitymentioning

confidence: 99%

Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure

Alli

Holzworth

et al. 2019

American Journal of Physiology-Renal Physiology

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Circadian rhythms govern physiological functions and are important for overall health. The molecular circadian clock comprises several transcription factors that mediate circadian control of physiological function, in part, by regulating gene expression in a tissue-specific manner. These connections are well established, but the underlying mechanisms are incompletely understood. The overall goal of this study was to examine the connection among the circadian clock protein Period 1 (Per1), epithelial Na+ channel (ENaC), and blood pressure (BP) using a multipronged approach. Using global Per1 knockout mice on a 129/sv background in combination with a high-salt diet plus mineralocorticoid treatment, we demonstrated that loss of Per1 in this setting is associated with protection from hypertension. Next, we used the ENaC inhibitor benzamil to demonstrate a role for ENaC in BP regulation and urinary Na+ excretion in 129/sv mice. We targeted Per1 indirectly using pharmacological inhibition of Per1 nuclear entry in vivo to demonstrate altered expression of known Per1 target genes as well as a BP-lowering effect in 129/sv mice. Finally, we directly inhibited Per1 via genetic knockdown in amphibian distal nephron cells to demonstrate, for the first time, that reduced Per1 expression is associated with decreased ENaC activity at the single channel level.

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“…However, it has been speculated that some cytoplasmic factors might inhibit ENaCs when the channels are resident in the intact basolateral cell membrane; these factors would be lost when switching to outside-out patches, thereby producing channel activation. Recent findings suggest that filamin, a cytoplasmic protein that binds actin in the cytoskeleton, inhibits ENaC activity [89]. It is tempting to speculate that during patch excision, the cytoskeleton layer under the cell membrane gets disrupted and this may remove a stationary inhibitory effect exerted by intracellular protein filaments on ENaCs.…”

Section: Excised Membrane Patchesmentioning

confidence: 99%

Electrophysiology of Sodium Receptors in Taste Cells

Bigiani¹

2016

JBiSE

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Sodium intake is important to maintain proper osmolarity and volume of extracellular fluid in vertebrates. The ability to find sources of sodium ions for managing electrolyte homeostasis relies on the activity of the taste system to sense salt. Several studies have been performed to understand the mechanisms underlying Na+ reception in taste cells, the peripheral detectors for food chemicals. It is now generally accepted that Na+ interacts with specific ion channels in taste cell membrane, called sodium receptors. As ion channels, these proteins mediate transmembrane ion fluxes (that is, electrical currents) during their operation. Thus, a lot of information on the functional properties of sodium receptors has been obtained by using electrophysiological techniques. Here, I review our current knowledge on the biophysical and physiological features of these receptors obtained by applying the patch-clamp recording techniques to single taste cells

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Filamin Interacts with Epithelial Sodium Channel and Inhibits Its Channel Function

Abstract: Background: ENaC is critical in Na ϩ homeostasis and blood pressure, but how it is regulated by the cytoskeleton remains

Cited by 22 publications

References 36 publications

Critical Roles of Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) in Substrate Selectivity and Urate Transport

Critical Roles of Two Hydrophobic Residues within Human Glucose Transporter 9 (hSLC2A9) in Substrate Selectivity and Urate Transport

Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure

Electrophysiology of Sodium Receptors in Taste Cells

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