Filter cubes with built‐in ultrabright light‐emitting diodes as exchangeable excitation light sources in fluorescence microscopy

Moser, Christoph; Mayr, Torsten; Klimant, Ingo

doi:10.1111/j.1365-2818.2006.01581.x

Cited by 20 publications

(13 citation statements)

References 9 publications

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“…A mercury arc lamp produces a lot of heat and harmful ultraviolet light that can damage cells. Alternatively, widening the narrow coherent laser beam in the focal plane results in a nonuniform irradiation pattern called speckles (Martin et al ., 2005; Merryman, 1999; Moser et al ., 2006). In addition, a commonly used strategy to improve the fluorescence signals is increasing the intensity of excitation light or the concentration of the fluorescent dyes, e.g.…”

Section: Introductionmentioning

confidence: 99%

Long‐term monitoring of live cell proliferation in presence of PVP‐Hypericin: a new strategy using ms pulses of LED and the fluorescent dye CFSE

Penjweini

Loew

Hamblin

et al. 2011

Journal of Microscopy

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Summary During fluorescent live cell imaging it is critical to keep excitation light dose as low as possible, especially in the presence of photosensitizer drugs, which generate free radicals upon photobleaching. During fluorescent imaging, stress by excitation and free radicals induces serious cell damages that may arrest the cell cycle. This limits the usefulness of the technique for drug discovery, when prolonged live cell imaging is necessary. This paper presents a strategy to provide gentle experimental conditions for dynamic monitoring of the proliferation of human lung epithelial carcinoma cells (A549) in the presence of the photosensitizer Polyvinylpyrrolidone-Hypericin. The distinctive strategy of this paper is based on the stringent environmental control and optimizing the excitation light dose by (i) using a low-power pulsed blue light-emitting diode with short pulse duration of 1.29 ms and (ii) adding a nontoxic fluorescent dye called carboxyfluorescein-diacetate-succinimidyl-ester (CFSE) to improve the fluorescence signals. To demonstrate the usefulness of the strategy, fluorescence signals and proliferation of dual-marked cells, during 5-h fluorescence imaging under pulsed excitation, were compared with those kept under continuous excitation and nonmarked reference cells. The results demonstrated 3% cell division and 2% apoptosis due to pulsed excitation compared to no division and 85% apoptosis under the continuous irradiation. Therefore, our strategy allows live cell imaging to be performed over longer time scales than with conventional continuous excitation.

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Section: Introductionmentioning

confidence: 99%

Long‐term monitoring of live cell proliferation in presence of PVP‐Hypericin: a new strategy using ms pulses of LED and the fluorescent dye CFSE

Penjweini

Loew

Hamblin

et al. 2011

Journal of Microscopy

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“…20-50 nm, when used as excitation sources in LED-IF for optimal performance and low baseline noise they have to be combined with low-pass filters [45,51,52].…”

Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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Graphical Abstract (please choose)A new generation of integrated miniaturized fibre-coupled solid-state light sources based on microfabricated light emitting diode micro-array (µ-LED), micropackaged with a custom band-pass optical interference filter deposited at the end of an optical fibre, is presented and in this work and demonstrated as excitation light source for fluorescence detection in capillary electrophoresis. ALTERNATIVE IMAGES: Key words:Micro-light emitting diode array; light source; optical fibre; capillary electrophoresis; fluorescence detection; separations. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 *Manuscript (including figures, tables, text graphics and associated captions) Click here to view linked References

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“…LEDs, even when mounted on heat sinks, are usually much smaller than other light sources. Some small LEDs are powerful enough to be mounted inside a microscope (as opposed to in an external lamphouse), directly under the specimen, or even on a live animal (Moser et al 2006;Huber et al 2007;Lang et al 2008). LEDs can be used in cold environments in which other illumination systems may have difficulties.…”

Section: Comparing Leds With Other Illumination Sourcesmentioning

confidence: 99%

Light-Emitting Diodes for Biological Microscopy

Sato¹,

Murthy²

2012

Cold Spring Harb Protoc

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Various technological advances have made imaging an increasingly useful tool in the life sciences. Imaging techniques that move away from the limitations of wide-field microscopy have allowed deeper, higher-resolution imaging of thick biological tissue. Even within wide-field microscopy, advancements such as structured and sheet illumination, as well as improvements of biological probes, have led to better visualization of cells and their subcellular structures. The illumination source for wide-field microscopy, however, has remained relatively unchanged for decades, relying mainly on xenon, mercury, and halogen lamps. Light-emitting diodes (LEDs) have existed for more than 80 years, but their spectral range and output light flux have only recently become large enough for use in biological applications. This article presents the basic information necessary to build and to use an LED-based illumination source for microscopy. It also provides some useful resources about LED advancements. Although commercial LED-based illuminators for microscopy are available, custombuilt illumination systems can incorporate the latest LED chips into microscopes more quickly and inexpensively than is possible through the retail market.

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Filter cubes with built‐in ultrabright light‐emitting diodes as exchangeable excitation light sources in fluorescence microscopy

Cited by 20 publications

References 9 publications

Long‐term monitoring of live cell proliferation in presence of PVP‐Hypericin: a new strategy using ms pulses of LED and the fluorescent dye CFSE

Long‐term monitoring of live cell proliferation in presence of PVP‐Hypericin: a new strategy using ms pulses of LED and the fluorescent dye CFSE

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Light-Emitting Diodes for Biological Microscopy

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