In order to explore the influence of different standard light sources on the digitization of ruby color, D65 and A standard illuminants were taken as the research objects. Based on the relative spectral power of the two light sources, the influence of different standard illuminants on the results was calculated and analyzed by CIE 1976 L* a* b* color model. The experimental results show that the relative spectral power distribution of the D65 standard illuminant is relatively uniform, and the L* a* b* color parameters under the light source can be collected more accurately, objectively and truly. Therefore, the D65 standard light source is more practical for the digitization of ruby color.