Filter paper–based insulin-like growth factor assay

Jensen, Anne K.; Coleman, Carrie M.; Stokes, David; Ying, Gui‐Shuang; Huang, Jiayan; Kuhn, Ivy Fenton; Binenbaum, Gil

doi:10.1016/j.jaapos.2015.03.022

Cited by 3 publications

(2 citation statements)

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“…Filter paper samples were frozen and later thawed in batches for sample extraction and IGF-1 assays using an enzyme-linked immunosorbent assay kit from R&D Systems (Minneapolis, MN) in a previously validated manner. 35 For statistical analysis, subjects were stratified into one of three ROP outcome groups by worst ROP stage in either eye: no ROP, mild to moderate (ROP stage 1 or 2), or severe (ROP stage 3 or worse). Weekly IGF-1 levels were compared among these groups by PMA week.…”

Section: Methodsmentioning

confidence: 99%

Postnatal serum insulin-like growth factor i and retinopathy of prematurity

Jensen

Ying

Huang³

et al. 2015

Journal of American Association for Pediatric Ophthalmology and

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Section: Methodsmentioning

confidence: 99%

Postnatal serum insulin-like growth factor i and retinopathy of prematurity

Jensen

Ying

Huang³

et al. 2015

Journal of American Association for Pediatric Ophthalmology and

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Section: Methodsmentioning

confidence: 99%

Postnatal Serum Insulin-Like Growth Factor I and Retinopathy of Prematurity

Jensen

Ying

Huang³

et al. 2017

Retina

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INTRODUCTION Low serum insulin-like growth factor 1 (IGF-1) has been associated with development of severe retinopathy of prematurity (ROP), but no U.S. studies have been reported. We sought to determine the relationship between postnatal serum IGF-1 levels and severe ROP in a racially diverse U.S. cohort. METHODS Prospective cohort study of 74 infants with birth weight (BW) <1251g and a known ROP outcome at 3 Philadelphia hospitals. Weekly postnatal filter-paper bloodspot IGF-1 assays) were measured through 42 weeks postmenstrual age (PMA). RESULTS The cohort included 20 Caucasian, 45 black, 2 Asian, and 9 other infants; median gestational age (GA) 27.6 weeks (range 23-33); median BW 975g (range 490-1250). During PMA weeks 28 to 33, mean IGF-1 was 20.0 ng/mL(SE 0.52) for no ROP (n=46), 18.0(0.49) for stage 1 or 2 (n=23), and 17.0(0.70) for stage 3 (n=5, 2 lasered) (p=0.003). Adjustment for BW and GA showed similar results. CONCLUSION Presence and timing of an association between low postnatal serum IGF and ROP in a racially diverse U.S. sample were found to be consistent with European cohorts. This association provides the pathophysiological basis for growth-based predictive models, which could improve efficiency of ROP screening.

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