1994
DOI: 10.1128/jb.176.3.748-755.1994
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FimH family of type 1 fimbrial adhesins: functional heterogeneity due to minor sequence variations among fimH genes

Abstract: We recently reported that the type 1-fimbriated Escherichia coli strains CSH-50 and HB101(pPKIA), both K-12 derivatives, have different patterns of adhesion to yeast mannan, human plasma fibronectin, and fibronectin derivatives, suggesting functional heterogeneity of type 1 fimbriae. In this report, we provide evidence that this functional heterogeneity is due to variations in the fimH genes. We also investigated functional heterogeneity among clinical isolates and whether variation infimH genes accounts for d… Show more

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Cited by 147 publications
(170 citation statements)
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“…Yeast Aggregation Assay-To analyze type 1 fimbriae expression, the mutant strains were subjected to yeast aggregation assay as described previously (40). In short, the mutants were cultured overnight, and their absorbance was adjusted to 0.4 at 530 nm using sterile PBS.…”
Section: Methodsmentioning
confidence: 99%
“…Yeast Aggregation Assay-To analyze type 1 fimbriae expression, the mutant strains were subjected to yeast aggregation assay as described previously (40). In short, the mutants were cultured overnight, and their absorbance was adjusted to 0.4 at 530 nm using sterile PBS.…”
Section: Methodsmentioning
confidence: 99%
“…Loss of the pKOBEGApra plasmid was verified by the inability of the mutant to grow on LB agar plates containing apramycin. Correct allelic exchange was verified by PCR analysis using combinations of Sokurenko et al (1994) primers inside the kan gene, K1 and K2 (Datsenko & Wanner, 2000), and primers flanking the deleted mrkD gene, UpmrkA (59-CCA-CCGCAAAGGCAGATTCAGACT-39) and DwmrkF (59-TTACATT-TCCCGCCATTCCCATTA-39).…”
Section: Methodsmentioning
confidence: 99%
“…P-fimbriae have been demonstrated to bind fibronectin (Westerlund et al, 1991) as well as type 1 fimbriae, whose ability to bind fibronectin HBD and GBD is strain-dependent and results of sequence heterogeneity in fimbrilin FimH (Sokurenko et al, 1994). In FimA, the binding motif was identified (Murakami et al, 1996).…”
Section: Bacterial Cell Surface Supramolecular Protein Structures Intmentioning
confidence: 99%