BACKGROUND: In this study, the feasibility of an aerobic granular sequencing batch reactor (AGSBR) for elimination of nutrients at low dissolved oxygen (DO) level was investigated. It was fed by a mixture of treated and raw cattle manure wastewater. The behaviour of the AGSBR was assessed at different chemical oxygen demand (COD)/NH 4 + -N ratios, feeding strategies (dump-fill and slow-rate feeding) and hydraulic retention times (24, 32 and 48 h). For a duration of 140 days, the capability of the AGSBR for reducing COD, ammonia and total phosphorus (TP) contents was studied. At DO concentrations of 0.3-1.0 mg L −1 , the effect of different COD/NH 4 + ratios (100:3-100:10) was evaluated.
RESULTS:A COD/NH 4 + -N ratio of 100:10 resulted in higher nitrification rate (0.0714 gN gMLVSS −1 day −1 ) compared to a ratio of >100:3 (0.0175 gN gMLVSS −1 day −1 ). The active granular sludge in dump-filling mode revealed better settling properties compared to the slow feeding rate. Microscopic observation of aerobic granules showed that the core of granules was dominantly occupied by filamentous microorganisms. Finally, at optimum conditions, removal efficiencies of NH 4 + -N, TP and COD were 99.9, 99 and 79%, respectively. CONCLUSIONS: Applying the AGSBR at low DO is promising as a cost-effective technique for the secondary treatment of livestock wastewaters. /jctb 60.5% when COD/TP was changed from 54:1 (run 77) to COD/TP of 133:1 (run 82). Besides, TP removal efficiency reached to its maximum level when the steady state occurred in ending runs. This might be due to the full dominance of phosphate-accumulating