Finding NEMO in preeclampsia

Sakowicz, Agata; Hejduk, Paulina; Pietrucha, Tadeusz; Nowakowska, Magdalena; Płuciennik, Elżbieta; Pospiech, Karolina; Gach, Agnieszka; Rybak-Krzyszkowska, Magda; Sakowicz, B.; Kamiński, Marek; Krasomski, Grzegorz; Biesiada, Lidia

doi:10.1016/j.ajog.2015.11.002

Cited by 13 publications

(22 citation statements)

References 37 publications

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“…Müller-Rischart and colleagues33 demonstrated that the OPA1 gene is a target of NF-κB-responsive promoter elements (e.g. NEMO–NF-κB essential modulator) which is upregulated in stressful conditions, particularly in PE34. We observed that in the eoPE group, OPA1 expression was significantly higher compared to control, for both transcript and protein levels.…”

Section: Discussionmentioning

confidence: 47%

Mitochondrial role in adaptive response to stress conditions in preeclampsia

Vishnyakova

Володина

Тарасова

et al. 2016

Sci Rep

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Preeclampsia (PE) is a pregnancy-specific syndrome, characterized in general by hypertension with proteinuria or other systemic disturbances. PE is the major cause of maternal and fetal morbidity and mortality worldwide. However, the etiology of PE still remains unclear. Our study involved 38 patients: 14 with uncomplicated pregnancy; 13 with early-onset PE (eoPE); and 11 with late-onset PE (loPE). We characterized the immunophenotype of cells isolated from the placenta and all biopsy samples were stained positive for Cytokeratin 7, SOX2, Nestin, Vimentin, and CD44. We obtained a significant increase in OPA1 mRNA and protein expression in the eoPE placentas. Moreover, TFAM expression was down-regulated in comparison to the control (p < 0.01). Mitochondrial DNA copy number in eoPE placentas was significantly higher than in samples from normal pregnancies. We observed an increase of maximum coupled state 3 respiration rate in mitochondria isolated from the placenta in the presence of complex I substrates in the eoPE group and an increase of P/O ratio, citrate synthase activity and decrease of Ca2+-induced depolarization rate in both PE groups. Our results suggest an essential role of mitochondrial activity changes in an adaptive response to the development of PE.

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Section: Discussionmentioning

confidence: 47%

Mitochondrial role in adaptive response to stress conditions in preeclampsia

Vishnyakova

Володина

Тарасова

et al. 2016

Sci Rep

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“…The procedure concerning the protection and storage of placenta fragments is described elsewhere [25]. …”

Section: Methodsmentioning

confidence: 99%

“…The obtained DNA was stored at -20°C for further analysis. RNA isolation and transcription into cDNA was described elsewhere [25]. …”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the level of NEMO gene expression in placentas from pregnancies complicated by preeclampsia is significantly lower than observed in non-complicated pregnancies. NEMO level may hence be one of the reasons for the abundance of preeclamptic placental apoptosis [25]. …”

Section: Introductionmentioning

confidence: 99%

“…In contrast to placentas derived from pregnancies complicated by hypertension and proteinuria, a higher level of NEMO gene expression has been observed in the blood of preeclamptic women (according to 1A, 1B, 1C transcripts and all transcripts together—Total NEMO) and their children (according to 1A transcript) [25]. Differences in the expression of the IKKγ gene in the tissues of preeclamptic and healthy women and their children may be related to the existence of some variation in the NEMO coding gene.…”

Section: Introductionmentioning

confidence: 99%

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Double hit of NEMO gene in preeclampsia

et al. 2017

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The precise etiology of preeclampsia is unknown. Family studies indicate that both genetic and environmental factors influence its development. One of these factors is NFkB, whose activation depends on NEMO (NFkB essential modulator. This is the first study to investigate the association between the existence of single nucleotide variant of the NEMO gene and the appearance of preeclampsia. A total of 151 women (72 preeclamptic women and 79 controls) and their children were examined. Sanger sequencing was performed to identify variants in the NEMO gene in the preeclamptic mothers. The maternal identified variants were then sought in the studied groups of children, and in the maternal and child controls, using RFLP-PCR. Real-time RT-PCR was performed to assess NEMO gene expression in maternal blood, umbilical cord blood and placentas. The sequencing process indicated the existence of two different variants in the 3'UTR region of the NEMO gene of preeclamptic women (IKBKG:c.*368C>A and IKBKG:c.*402C>T). The simultaneous occurrence of the TT genotype in the mother and the TT genotype in the daughter or a T allele in the son increased the risk of preeclampsia development 2.59 fold. Additionally, we found that the configuration of maternal/fetal genotypes (maternal TT/ daughter TT or maternal TT/son T) of IKBKG:c.*402C/T variant is associated with the level of NEMO gene expression. Our results showed that, the simultaneous occurrence of the maternal TT genotype (IKBKG:c.*402C>T variants) and TT genotype in the daughter or T allele in the son correlates with the level of NEMO gene expression and increases the risk of preeclampsia development. Our observations may offer a new insight into the genetic etiology and pathogenesis of preeclampsia.

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NF-κB and Related Autoimmune and Autoinflammatory Diseases

Freigeh,

Michniacki

2023

Rheumatic Disease Clinics of North America

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Finding NEMO in preeclampsia

Cited by 13 publications

References 37 publications

Mitochondrial role in adaptive response to stress conditions in preeclampsia

Mitochondrial role in adaptive response to stress conditions in preeclampsia

Double hit of NEMO gene in preeclampsia

NF-κB and Related Autoimmune and Autoinflammatory Diseases

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