Finding of a novel fungal immunomodulatory protein coding sequence in Ganoderma australe

Muñoz, Andrea González; Orozco, Kelly Johana Botero; Gartner, Germán Ariel López

doi:10.15446/rev.colomb.biote.v16n2.38747

Cited by 7 publications

(3 citation statements)

References 28 publications

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“…Amino acid sequences of FIPs, listed in Supplementary Table S2, were downloaded from NCBI (https://www.ncbi.nlm.nih.gov (accessed on 9 October 2022)). To identify immunomodulatory protein homologous genes in H. marmoreus, c13717 amino acid sequence was used as a bait [44], and a local BLAST search was performed to search for immunomodulatory protein homologous genes from the sequenced H. marmoreus genome [43].…”

Section: Bioinformatics Analysismentioning

confidence: 99%

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Wang

et al. 2023

Molecules

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A novel fungal immunomodulatory protein (FIP), identified as FIP-hma, was discovered in the genome of an edible mushroom Hypsizygus marmoreus. Bioinformatics analysis suggested FIP-hma contained the cerato-platanin (CP) conserved domain and was categorized into Cerato-type FIP. In phylogenetic analysis, FIP-hma was clustered into a new branch of the FIP family, displaying large system divergence from most of the other FIPs. The higher gene expression of FIP-hma was observed during the vegetative growth stages than that during the reproductive growth stages. In addition, the cDNA sequence of FIP-hma was cloned and successfully expressed in Escherichia coli (E. coli) BL21(DE3). The recombinant protein of FIP-hma (rFIP-hma) was neatly purified and isolated by Ni-NTA and SUMO-Protease. The iNOS, IL-6, IL-1β, and TNF-α levels of RAW 264.7 macrophages were upregulated by rFIP-hma, indicating its activation of an immune response by regulating central cytokines. No cytotoxic effects were observed in an MTT test. The findings of this work discovered a novel immunoregulatory protein from H. marmoreus, provided a systematic bioinformatic profile, suggested an effective approach for its heterologous recombinant production, and reported its potent immunoregulatory activity in macrophages. This study sheds light on the physiological function research of FIPs and their further industrial utilization.

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Section: Bioinformatics Analysismentioning

confidence: 99%

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Wang

et al. 2023

Molecules

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“…In a previous work we obtained the complete sequence of the transcriptome of cultured cells (mycelium) from G. australe with the aim of a preliminary analysis of the transcriptome and finding genes of medical importance [17]. From this work we identified a candidate cDNA sequence belonging to the fungal immunomodulatory protein (FIP) family, which has shown promising results, tested and documented exhaustively for cancer therapy and other diseases that compromise the immune system [18,19] The transcriptome studied here consists of 712683 reads, 10,257 contigs (putative exons), 9.109 Isotigs (consensus sequences encoding putative proteins) and 8.170 isogroups (potential genes) [17].…”

Section: Introductionmentioning

confidence: 99%

“…From this work we identified a candidate cDNA sequence belonging to the fungal immunomodulatory protein (FIP) family, which has shown promising results, tested and documented exhaustively for cancer therapy and other diseases that compromise the immune system [18,19] The transcriptome studied here consists of 712683 reads, 10,257 contigs (putative exons), 9.109 Isotigs (consensus sequences encoding putative proteins) and 8.170 isogroups (potential genes) [17]. Based on these data it was proceeded with the identification of new sequences of biomedical interest, including enzymes compromised in the ganoderic acids pathway.…”

Section: Introductionmentioning

confidence: 99%

Identification of a Putative Ganoderic Acid Pathway Enzyme in a Ganoderma Australe Transcriptome by Means of a Hidden Markov Model

López-Gartner

Agudelo-Valencia

Castaño

et al. 2015

Advances in Intelligent Systems and Computing

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Ganoderma australe is a fungus widely used as a traditional medicine mainly in Eastern countries, but not studied in silico at the genomic level. This species is probably related to other well characterized fungus with similar properties, which may facilitate gene finding through comparative molecular analysis using appropriated bioinformatics tools. This paper aims to present a preliminary analysis of a G. australe transcriptome through some computational biology techniques implementing Hidden Markov Models (HMM) in order to predict a key putative enzyme (lanosterol synthase, EC 5.4.99.7) involved in the metabolic pathway of triterpenoids of therapeutic interest. The findings suggest that the HMM approach results more efficient than traditional comparisons by homology based on

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Molecular cloning, codon-optimized gene expression, and bioactivity assessment of two novel fungal immunomodulatory proteins from Ganoderma applanatum in Pichia

Zhou

Guan

Duan

et al. 2018

Appl Microbiol Biotechnol

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Fungal immunomodulatory proteins (FIPs) have been identified from a series of fungi, especially in Ganoderma species. However, little is known about the FIPs from G. applanatum. In this study, two novel FIP genes, termed as FIP-gap1 and FIP-gap2, were cloned from G. applanatum, characterized and functionally expressed after codon optimization in Pichia pastoris GS115. Results showed that FIP-gap1 and FIP-gap2 comprised 342-bp encoding peptides of 113 amino acids, which shared a high homology with other Ganoderma FIPs. The yield of recombinant FIP-gap1 and FIP-gap2 increased significantly after codon optimization and reached 247.4 and 197.5 mg/L, respectively. Bioactivity assay in vitro revealed that both rFIP-gap1 and rFIP-gap2 could agglutinate mouse, sheep, and human red blood cells. Besides, rFIP-gap1 and rFIP-gap2 obviously stimulated the proliferation of mouse splenocytes and enhanced IL-2 and IFN-γ release. Cytotoxicity detection indicated that IC of rFIP-gap1 towards A549 and HeLa cancer cells were 29.89 and 8.34 μg/mL, respectively, whereas IC of rFIP-gap2 to the same cancer cells were 60.92 and 41.05 μg/mL, respectively. Taken together, novel FIP gaps were cloned and functionally expressed in P. pastoris, which can serve as feasible and stable resources of rFIP gaps for further studies and potential applications.

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Finding of a novel fungal immunomodulatory protein coding sequence in Ganoderma australe

Cited by 7 publications

References 28 publications

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Characterization, Recombinant Production, and Bioactivity of a Novel Immunomodulatory Protein from Hypsizygus marmoreus

Identification of a Putative Ganoderic Acid Pathway Enzyme in a Ganoderma Australe Transcriptome by Means of a Hidden Markov Model

Molecular cloning, codon-optimized gene expression, and bioactivity assessment of two novel fungal immunomodulatory proteins from Ganoderma applanatum in Pichia

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