Findings of multiple HPV genotypes in cervical carcinoma are associated with poor cancer-specific survival in a Swedish cohort of cervical cancer primarily treated with radiotherapy

Kaliff, Malin; Sorbe, Bengt; Mordhorst, Louise Bohr; Helenius, Gisela; Karlsson, Mats G.; Lillsunde-Larsson, Gabriella

doi:10.18632/oncotarget.24666

Cited by 40 publications

(36 citation statements)

References 36 publications

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“…To date, this method has only been used on cervical samples. [22][23][24] To the best of our knowledge, only 1 study has evaluated the Anyplex on FFPE samples but it included very few OPSCC cases. 25 In this context, we evaluated the performance of the Anyplex in detecting and genotyping HPV-DNA on a large series of FFPE OPSCCs.…”

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confidence: 99%

Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas

Rollo¹,

Donà

Pichi³

et al. 2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— The improved survival and better response to treatments of human papillomavirus (HPV)–related oropharyngeal squamous cell carcinoma (OPSCC) highlight the need for effective tools in evaluating HPV status on formalin-fixed, paraffin-embedded (FFPE) cancer tissues. To date, there is no agreement regarding the most appropriate method for HPV testing on FFPE materials. Objective.— We aimed to investigate the performance of the Anyplex II HPV28 (Anyplex) on FFPE OPSCC tissues and to compare it with 2 other methods for HPV-DNA detection and p16 overexpression. Design.— One hundred sixty FFPE OPSCCs were evaluated, which had already been analyzed with the INNO-LiPA HPV assay, Xpert HPV assay, and p16 immunostaining. Results.— All the samples but 1 provided valid results with the Anyplex, which showed the highest HPV detection rate and a good concordance with all the other methods (κ = 0.75, 95% CI, 0.65–0.85 versus INNO-LiPA; κ = 0.80, 95% CI, 0.70–0.89 versus Xpert; κ = 0.76, 95% CI, 0.65–0.86 versus p16). Moreover, the HPV-driven fraction, based on HPV-DNA and p16 double positivity, was higher with Anyplex (83 of 159, 52.2%) than with the other 2 assays, that is, 78 of 156 (50.0%) for INNO-LiPA and 80 of 160 (50.0%) for Xpert. Conclusions.— Anyplex II HPV28 showed a higher HPV detection rate and HPV-associated fraction than the other methods used. This assay is suitable for HPV detection in archival OPSCC tissues.

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confidence: 99%

Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas

Rollo¹,

Donà

Pichi³

et al. 2019

Archives of Pathology &Amp; Laboratory Medicine

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“…Among the numerous subtypes of this virus, the most common and important high-risk type is HPV16. At least 50% of cervical cancers are associated with HPV16 [28]. HPV16 E6 protein is one of the major proteins regulating the life cycle of the virus and plays an important role in tumor cell development.…”

Section: Discussionmentioning

confidence: 99%

Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines

Hong¹,

Zhang²,

Liu³

et al. 2020

EJGO

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Background:In cervical cancer, matrix extracellular phosphoglycoprotein (MEPE) plays an important role of multidrug resistance, which is associated with miR-758. Mifepristone has anti-drug resistance effects, but whether mifepristone regulates the expression of multidrug resistance proteins via the pathway mediated by miR-758/MEPE is still unclear. Hela cells were induced by mifepristone (named as Hela/MIF cells). Then, matrix extracellular phosphoglycoprotein siRNA, and miR-758 mimics were transfected into HeLa/MIF cells. The sensitivity, clone forming ability, migration, and level of apoptosis of the cells in each group were respectively measured by CCK-8 assays, clone forming assays, transwell assays, and flow cytometry analysis. The targeting of miR-758 to MEPE was verified by dual-luciferase assay. At last, the expression of MDR-1, MRP-1, and GST-π were detected by qPCR and western blot assays. Results: The induction by mifepristone not only decreased the sensitivity of Hela cells, but also up-regulated the expression of multidrug resistance proteins. On this basis, by increasing the expression of miR-758 or downregulating the expression of MEPE, the expression of multidrug resistance proteins decreased, while the sensitivity of Hela cells to mifepristone were improved and the level of Hela cells proliferation, colony forming, and invasion further decreased. Conclusions: The induction by mifepristone inhibited the proliferation of Hela cells, but the sensitivity of Hela cells increased. The mechanism may depend on the expression of multidrug resistance protein. This study shows that regulating the expression of miR-758 and MEPE can reduce the resistance of Hela cells to mifepristone, enhance the sensitivity, and further improve the inhibitory effect of mifepristone.

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“…Studies have shown that the most common HR HPV detected in carcinoma cases are HPV 16, 18, 31, 33, 39, and 45 (Muñoz 2000;Kraus et al, 2006;Parkin et al, 2008;Wohlmeister et al, 2016a). Kaliff et al, ( 2018) carried out a study and found that HPV 16 was the most prevalent genotype, followed by HPV 18: the two are the main genotypes detected in squamous cell carcinoma (Oliveira-Silva et al, 2011;Wohlmeister et al, 2016). The 12 other high-risk HPV genotypes (viz.,HPV 31,33,35,39,45,51,52,56,58,59,66,and 68) were highly prevalent in the present study; more in fact than HPV 16 or HPV 18.…”

Section: Discussionmentioning

confidence: 99%

Chlamydia Trachomatis Infection in High-Risk Human Papillomavirus Based on Cervical Cytology Specimen

Sangpichai

Patarapadungkit

Pientong

et al. 2019

Asian Pac J Cancer Prev

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Asian Pac J Cancer Prev, 20 (12), [3843][3844][3845][3846][3847] Human papillomavirus (HPV) infection in women are usually asymptomatic and most clear spontaneously within 1-2 years (Jenkins, 2007). Persistent infection with various high-risk types of HPV (HR HPV) often progresses to invasive cancer. HPV DNA can be detected in more than 99% of cervical cancer, of which 70% are caused by HR HPV16 and 18 (Douglas, 2016). HPV16 was the most common type found in cervical carcinoma in all regions. The other associated risk factors for developing cervical cancer are first coitus age, multiple sexual partners, cigarette smoking, sexually-transmitted disease (STD), immunosuppression, oral contraceptive use, and co-infection with other sexually-transmitted infection (STI) (Jhingran et al., 2014).Chlamydia trachomatis (C. trachomatis) is the most common bacterial STI worldwide with more than 1.4 AbstractObjective: High-risk human papillomavirus (HR HPV) was associated with the development of cervical cancer. Asymptomatic Chlamydia trachomatis (C. trachomatis) infection is the most common bacterial, sexually-transmitted infection. This study aimed to investigate the association of C. trachomatis in positive HR HPV and the cytological results from liquid-based cytology (LBC). Methods: 150 residual LBC specimens were collected; all of which had undergone cytology and HPV testing by Cobas. The samples were established as C. trachomatis using real-time PCR (RT-PCR) with Cryptic F/Cryptic R primers. Results: Of 150 positive HPV findings, the most common (72.7%, 109/150) were the 12 other HR HPVs (viz., 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68). The cervical cytology of those positive HR HPVs were mostly negative (70.0%, 105/150). The C. trachomatis infections in positive HR HPV were 16% (24/150) HPV. The analysis of the abnormal cytology revealed that 41.6% had C. trachomatis coinfection (C. trachomatis and HPV infection) viz., LSIL (20.8%), HSIL (12.5%), and ASC-US (8.3%). A comparison with positive HPV without C. trachomatis co-infection revealed that the highest prevalence was for LSIL, ASC-US, and HSIL (11.1%, 10.3%, and 6.4%, respectively). There was no difference between the abnormalities and negative cervical cytology with negative and positive C. trachomatis co-infection in HR HPV positive (p = 0.174). Conclusion: C. trachomatis infection was not significantly associated HR-HPV and abnormal cytology. This study confirms the increasing rate of C. trachomatis infection in asymptomatic women so routine screening for these infections has been suggested to (a) prevent complications such as the chronic pelvic pain associated with prolong infection and (b) reduce sexual transmission of the infection.

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Findings of multiple HPV genotypes in cervical carcinoma are associated with poor cancer-specific survival in a Swedish cohort of cervical cancer primarily treated with radiotherapy

Cited by 40 publications

References 36 publications

Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas

Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas

Mifepristone regulates the multidrug resistance via miR-758/MEPE of Hela cell lines

Chlamydia Trachomatis Infection in High-Risk Human Papillomavirus Based on Cervical Cytology Specimen

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