The article contains new information about the locations of rare and spreading species of flowering plants in Western Siberia and Southern Ural (Kurgan Region, Chelyabinsk Region, Tomsk Region, and Republic of Altai). Among them, four species (Bidens frondosa L., Lappula intermedia (Ledeb.) Popov, Melica altissima L., Verbascum lychnitis L.) are reported as new for the Tomsk Region, three species (Aquilegia vulgaris L., Dianthus barbatus L., Lepidium sativumL.) are new for Republic of Altai, two species (Hibiscus trionum L., Solanum physalifolium Rusby) are new for the Chelyabinsk Region. For seven more species (Achillea ptarmica L., Chaenorhinum minus (L.) Lange, Chenopodium acerifolium Andrz., Cyclachaena xanthiifolia (Nutt.) Fresen., Impatiens parviflora DC., Najas major All., Oberna cserei(Baumg.) Ikonn.) new locations are cited.