Fine mapping and characterization of the dominant gene SmFTSH10 conferring non-photosensitivity in eggplant (Solanum melongena L.)

He, Yongjun; Li, ShaoHang; Dong, Yanxiao; Zhang, XinTong; Li, DaLu; Liu, Yang; Chen, Huoying

doi:10.1007/s00122-022-04078-z

Cited by 11 publications

(8 citation statements)

References 31 publications

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“…Recent studies have found that the candidate gene related to non-photosensitive anthocyanin biosynthesis in eggplant is SmFTSH10 (He et al, 2022), which was physically close to, but outside the candidate interval 7.7Mb-11.7Mb (Figure 3C) for less-photosensitive anthocyanin biosynthesis in this study, indicating less-and non-photosensitive anthocyanin biosynthesis in eggplant may be controlled by different locus.…”

Section: Gene Identification Related To Lessphotosensitive Anthocyani...supporting

confidence: 55%

“…The number in the box indicates the FPKM value. 1A), whereas there was no significant difference for non-photosensitive materials (He et al, 2019;He et al, 2022).…”

Section: Gene Identification Related To Lessphotosensitive Anthocyani...mentioning

confidence: 89%

“…However, the above studies did not distinguish materials as less-photosensitive or non-photosensitive materials. When comparing the phenotypic differences between the less-photosensitive or non-photosensitive materials, it was observed that under bagging conditions, fruit coloration became significantly lighter for less-photosensitive materials ( Figure 1A ), whereas there was no significant difference for non-photosensitive materials ( He et al., 2019 ; He et al., 2022 ). Recent studies have found that the candidate gene related to non-photosensitive anthocyanin biosynthesis in eggplant is SmFTSH10 ( He et al., 2022 ), which was physically close to, but outside the candidate interval 7.7Mb-11.7Mb ( Figure 3C ) for less-photosensitive anthocyanin biosynthesis in this study, indicating less- and non-photosensitive anthocyanin biosynthesis in eggplant may be controlled by different locus.…”

Section: Discussionmentioning

confidence: 99%

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Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

Luo,

Molthoff,

et al. 2023

Front. Plant Sci.

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Eggplant (Solanum melongena L.) is a highly nutritious and economically important vegetable crop. However, the fruit peel of eggplant often shows poor coloration owing to low-light intensity during cultivation, especially in the winter. The less-photosensitive varieties produce anthocyanin in low light or even dark conditions, making them valuable breeding materials. Nevertheless, genes responsible for anthocyanin biosynthesis in less-photosensitive eggplant varieties are not characterized. In this study, an EMS mutant, named purple in the dark (pind), was used to identify the key genes responsible for less-photosensitive coloration. Under natural conditions, the peel color and anthocyanin content in pind fruits were similar to that of wildtype ‘14-345’. The bagged pind fruits were light purple, whereas those of ‘14-345’ were white; and the anthocyanin content in the pind fruit peel was significantly higher than that in ‘14-345’. Genetic analysis revealed that the less-photosensitive trait was controlled by a single dominant gene. The candidate gene was mapped on chromosome 10 in the region 7.72 Mb to 11.71 Mb. Thirty-five differentially expressed genes, including 12 structural genes, such as CHS, CHI, F3H, DFR, ANS, and UFGT, and three transcription factors MYB113, GL3, and TTG2, were identified in pind using RNA-seq. Four candidate genes EGP21875 (myb domain protein 113), EGP21950 (unknown protein), EGP21953 (CAAX amino-terminal protease family protein), and EGP21961 (CAAX amino-terminal protease family protein) were identified as putative genes associated with less-photosensitive anthocyanin biosynthesis in pind. These findings may clarify the molecular mechanisms underlying less-photosensitive anthocyanin biosynthesis in eggplant.

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Section: Gene Identification Related To Lessphotosensitive Anthocyani...supporting

confidence: 55%

“…The number in the box indicates the FPKM value. 1A), whereas there was no significant difference for non-photosensitive materials (He et al, 2019;He et al, 2022).…”

Section: Gene Identification Related To Lessphotosensitive Anthocyani...mentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

Luo,

Molthoff,

et al. 2023

Front. Plant Sci.

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“…As research progresses, various algorithms and software have been developed for QTL detection [21][22][23]. The BSA method is not only widely used in crops such as rice, but also widely used in vegetables [28], ornamental plants [36], and evenly highly heterozygous materials such as potatoes [14,16]. These studies have identified several QTLs that are closely linked to important agronomic traits and developed closely linked molecular markers, providing a basis for marker-assisted breeding.…”

Section: Discussionmentioning

confidence: 99%

“…The availability of high-quality reference genomes of eggplant [25,26] has provided valuable resources for such studies. The combination of high-quality reference genomes and BSA-seq analysis has facilitated the identification of candidate genes and genomic regions underlying important traits in eggplant, such as rind color, non-photosensitivity, and plant height [27][28][29]. These studies have contributed to the understanding of the genetic basis of these traits in eggplant, offering valuable insights for future breeding and gene cloning endeavors.…”

Section: Introductionmentioning

confidence: 99%

QTL Mapping for Bacterial Wilt Resistance in Eggplant via Bulked Segregant Analysis Using Genotyping by Sequencing

Xiao,

Lin,

Nie

et al. 2024

Agronomy

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The bacterial wilt disease caused by Ralstonia solanacearum is a significant threat to eggplant production. Breeding and promoting resistant varieties is one of the most effective methods to manage bacterial wilt. Conducting QTL (quantitative trait locus) mapping of resistant genes can substantially enhance the breeding of plant resistance to bacterial wilt. In this study, a population of 2200 F2 individuals derived from resistant and susceptible materials was utilized to establish extreme resistance and susceptibility pools. Following resequencing analysis of the parents and extreme pools, the QTL were examined using the DEEP-BSA software and QTLseqr R package (version 0.7.5.2). The results revealed that the detection of 10 QTL sites on chromosomes 5, 8, 9, and 11 by the five algorithms of the DEEP-BSA software. Additionally, the candidate region of 62 Mb–72 Mb on chromosome 5 was identified in all five algorithms of the DEEP-BSA software, as well as by the QTLseqr R package. Subsequent gene annotation uncovered 276 genes in the candidate region of 62 Mb–72 Mb on chromosome 5. Additionally, RNA-seq results indicated that only 13 genes had altered expression levels following inoculation with R. solanacearum in the resistant materials. Based on the expression levels, SMEL4_05g015980.1 and SMEL4_05g016110.1 were identified as candidate genes. Notably, SNP annotation identified a non-synonymous mutation in the exonic region of SMEL4_05g015980.1 and a variant in the promoter region of SMEL4_05g016110.1. The research findings have practical significance for the isolation of bacterial wilt resistance genes in eggplant and the development of resistance to bacterial wilt varieties in eggplant.

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Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum

Zhang,

Bosland,

et al. 2024

Protoplasma

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Fine mapping and characterization of the dominant gene SmFTSH10 conferring non-photosensitivity in eggplant (Solanum melongena L.)

Cited by 11 publications

References 31 publications

Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

Identification of candidate genes associated with less-photosensitive anthocyanin phenotype using an EMS mutant (pind) in eggplant (Solanum melongena L.)

QTL Mapping for Bacterial Wilt Resistance in Eggplant via Bulked Segregant Analysis Using Genotyping by Sequencing

Mapping of resistance genes to powdery mildew based on DNA re-sequencing and bulk segregant analysis in Capsicum

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