Fine mapping and pyramiding of brown planthopper resistance genes QBph3 and QBph4 in an introgression line from wild rice O. officinalis

Hu, Jie; Xiao, Cong; Cheng, Ming Huei; Gao, Guanjun; Zhang, Qinglu; He, Yuqing

doi:10.1007/s11032-015-0228-2

Cited by 43 publications

(18 citation statements)

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“…Lv et al 35. and Hu et al 22. reported that Bph15 and QBph4 were located proximally to Bph17 , thus differing from the present findings.…”

Section: Discussioncontrasting

confidence: 99%

“…QBph3 has a number of amino acid substitutions compared with Bph14. Hu et al 22. reported that QBph3 and Bph14 were tightly linked on chromosome 3 L, but the QBph3 -NIL showed a higher degree of resistance than Bph14 -NIL.…”

Section: Discussionmentioning

confidence: 99%

“…In our study, 13 BPH resistance genes from nine donors [RH ( Bph3 and Bph17 ), B5 ( Bph14 and Bph15 ), IR54751-1-2-44 ( QBph3 and QBph4 ), IR65482-4-136 ( Bph10 ), IR71033-121 ( Bph20 and Bph21 ), IR65482-7-216 ( Bph18 ), Swarnalata ( Bph6 ), Pokkali ( Bph9 ) and BR96 ( Bph24 )]11212223242526272829 were individually incorporated into cultivar (cv.) 9311 (an elite restorer parent for hybrids in China) by MABB, and 13 monogenic NILs were developed with enhanced BPH resistance.…”

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confidence: 99%

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Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

Sci Rep

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Brown planthopper (BPH) is the most destructive pest of rice in Asia. To date 29 BPH resistance genes have been identified, but only a few genes are being used in breeding due to inefficient markers for marker-assisted selection (MAS) and little knowledge of the real effects of the genes. In this study we individually transferred 13 genes or QTLs (Bph14, QBph3, QBph4, Bph17, Bph15, Bph20, Bph24, Bph6, Bph3, Bph9, Bph10, Bph18 and Bph21) into cultivar 9311 by marker assisted backcross breeding (MABB). Through positive and negative selection we narrowed the segments from donors containing Bph14, Bph15, Bph6 and Bph9 to 100–400 kb. Whole-genome background selection based on a high resolution SNP array was performed to maximize reconstitution of the recurrent parent genome (RPG 99.2–99.9%). All genes reduced BPH growth and development and showed antibiotic responses in seedlings. Based on genetic effects and amino acid sequences of genes in three clusters we inferred that Bph10 and Bph21 might be identical to Bph26, whereas Bph9 and Bph18 were different. Bph15 might be same with Bph17, but QBph4, Bph20 and Bph24 might be different. We believe that these NILs will be useful in rice BPH resistance research and breeding.

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“…Lv et al 35. and Hu et al 22. reported that Bph15 and QBph4 were located proximally to Bph17 , thus differing from the present findings.…”

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Xiao

et al. 2016

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“…Our results therefore indicate that hybrid rice is not inherently susceptible to herbivores, including S. furcifera , but that the CMS lines that are currently used in several modern rice breeding programs do have higher susceptibility to planthoppers and leafhoppers. This susceptibility should be carefully controlled, or counteracted using resistance sources (Fujita et al , ; Hu et al , , ) to avoid the development of susceptible hybrid varieties.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility and tolerance in hybrid and pure-line rice varieties to herbivore attack: biomass partitioning and resource-based compensation in response to damage

et al. 2016

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Hybrid rice has been noted for its susceptibility to insects and diseases compared to pure-line (conventional) rice varieties. We investigated herbivory by Nilaparvata lugens, Sogatella furcifera and Scirpophaga incertulas on replicated three-line hybrid sets (parental and hybrid lines) in field and greenhouse experiments. In a field experiment, caterpillar densities and stemborer damage was similar among hybrid and parental lines. In field and greenhouse experiments, the cytoplasmic male sterile (CMS)-lines and maintainer lines had higher densities of planthoppers (including N. lugens and S. furcifera) than restorer or hybrid lines likely because of their wild abortive CMS-lineage. High nitrogen levels increased plant mortality due to N. lugens, but often reduced mortality from S. furcifera and S. incertulas: this was similar between hybrid and pure-line varieties. The hybrids were generally more tolerant of herbivory (lower biomass reductions per unit weight of insect) than the inbred parental lines. The addition of nitrogen to both the hybrid and pure-line varieties had contrasting effects on tolerance depending on the nature of the attacking insect: fertiliser increased tolerance to S. furcifera (lower losses of yield and shoot biomass per mg insect) and S. incertulas (lower yield, shoot and root biomass loss) but fertiliser reduced tolerance to N. lugens (higher loss of root biomass and no effects on yield and shoot biomass loss). Our results indicate that hybrid rice is not physiologically more susceptible to herbivores than are pure-line varieties even under high nitrogen conditions, but does have higher tolerance to insect damage.

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“…indica and wild relatives of rice (Prahalada et al 2017 ; Guo et al 2018 ). Of them, 22 genes or QTLs ( Bph1 , Bph2/Bph26 , Bph3 , Bph6 , bph7 , Bph9 , Bph12 , Bph14 , Bph15 , Bph17 , Bph18 , Bph19 , Bph20 , Bph21 , Bph27 , Bph27(t) , Bph28(t) , bph29 , QBph3 , QBph4 , QBph4.2 , Bph31 and Bph32 ) have been fine-mapped (Cha et al 2008 ; Murai et al 2001 ; Jairin et al 2007 ; Qiu et al 2010 , 2012 , 2014 ; Zhao et al 2016 ; Du et al 2009 ; Lv et al 2014 ; Liu et al 2015 ; Jena et al 2006 ; Chen et al 2006 ; Rahman et al 2009 ; Huang et al 2013 ; He et al 2013 ; Wu et al 2014 ; Wang et al 2015 ; Hu et al 2015a , b ; Prahalada et al 2017 ; Ren et al 2016 ). However, only 8 genes ( Bph14 , Bph26 , Bph3 , bph29 , Bph32 , Bph18 , Bph9 and Bph6 ) have been isolated by map-based cloning (Du et al 2009 ; Tamura et al 2014 ; Liu et al 2015 ; Wang et al 2015 ; Ren et al 2016 ; Ji et al 2016 ; Zhao et al 2016 ; Guo et al 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and fine mapping of Bph33, a new brown planthopper resistance gene in rice (Oryza sativa L.)

Chang

Zou

et al. 2018

Rice

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BackgroundHost-plant resistance is the most desirable and economic way to overcome BPH damage to rice. As single-gene resistance is easily lost due to the evolution of new BPH biotypes, it is urgent to explore and identify new BPH resistance genes.ResultsIn this study, using F2:3 populations and near-isogenic lines (NILs) derived from crosses between two BPH-resistant Sri Lankan rice cultivars (KOLAYAL and POLIYAL) and a BPH-susceptible cultivar 9311, a new resistance gene Bph33 was fine mapped to a 60-kb region ranging 0.91–0.97 Mb on the short arm of chromosome 4 (4S), which was at least 4 Mb distant from those genes/QTLs (Bph12, Bph15, Bph3, Bph20, QBph4 and QBph4.2) reported before. Seven genes were predicted in this region. Based on sequence and expression analyses, a Leucine Rich Repeat (LRR) family gene (LOC_Os04g02520) was identified as the most possible candidate of Bph33. The gene exhibited continuous and stable resistance from seedling stage to tillering stage, showing both antixenosis and antibiosis effects on BPH.ConclusionThe results of this study will facilitate map-based cloning and marker-assisted selection of the gene.Electronic supplementary materialThe online version of this article (10.1186/s12284-018-0249-7) contains supplementary material, which is available to authorized users.

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Fine mapping and pyramiding of brown planthopper resistance genes QBph3 and QBph4 in an introgression line from wild rice O. officinalis

Cited by 43 publications

References 30 publications

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Development and evaluation of near-isogenic lines for brown planthopper resistance in rice cv. 9311

Susceptibility and tolerance in hybrid and pure-line rice varieties to herbivore attack: biomass partitioning and resource-based compensation in response to damage

Identification and fine mapping of Bph33, a new brown planthopper resistance gene in rice (Oryza sativa L.)

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