Fine Mapping of a Low-Temperature Germinability QTL<i>qLTG1</i>Using Introgression Lines Derived from<i>Oryza rufipogon</i>

Shim, Kyu-Chan; Kim, Sunha; Le, Anh Q.; Lee, Hyun‐Sook; Adeva, Cheryl; Jeon, Yun-A; Luong, Ngoc Ha; Kim, Woo Jin; Akhtamov, Mirjalol; Ahn, Sang-Nag

doi:10.9787/pbb.2019.7.2.141

Cited by 17 publications

(19 citation statements)

References 28 publications

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“…A total of 18 markers, including 15 Simple Sequence Repeat (SSR) and 3 Insertion-Deletion (InDel) markers were used to map QTLs ( Table S1 ). Three InDel markers (qLTG3-1_18D, qLTG10_InDel3, and qLTG10_InDel4) and one SSR marker (CRM22) were designed and used for mapping [ 27 , 28 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, five QTLs for low-temperature germinability, qLTG1 , qLTG3 , qLTG4 , qLTG10 , and qLTG11 were mapped using a BC 4 F 8 population including 96 introgression lines (ILs) derived from an interspecific cross between a Korean elite line Hwaseong and O. rufipogon [ 27 ]. Recently, qLTG1 was fine mapped using introgression lines and the new qLTG3-1 allele of the O. rufipogon was identified [ 28 , 29 ]. In the present study, we used two introgression lines, CR1517 and CR1518, which consistently exhibit higher LTG than Hwaseong due to O. rufipogon introgression segments in the Hwaseong genetic background, as parents.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Quantitative Trait Loci for Germination and Coleoptile Length under Low-Temperature Condition Using Introgression Lines Derived from an Interspecific Cross in Rice

Akhtamov

Adeva

Shim

et al. 2020

Genes

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Previously, five putative quantitative trait loci (QTLs) for low-temperature germination (LTG) have been detected using 96 BC3F8 lines derived from an interspecific cross between the Korean japonica cultivar “Hwaseong” and Oryza rufipogon. In the present study, two introgression lines, CR1517 and CR1518, were used as parents to detect additional QTLs and analyze interactions among QTLs for LTG. The F2 population (154 plants) along with parental lines, Hwaseong and O. rufipogon, were evaluated for LTG and coleoptile length under low-temperature conditions (13 °C). Among five QTLs for LTG, two major QTLs, qLTG1 and qLTG3, were consistently detected at 6 and 7 days after incubation. Three minor QTLs were detected on chromosomes 8 and 10. Two QTLs, qLTG10.1 and qLTG10.2, showing linkage on chromosome 10, exerted opposite effects with the Hwaseong allele at qLTG10.2 and the O. rufipogon allele at qLTG10.1 respectively, in turn, increasing LTG. Interactions among QTLs were not significant, implying that the QTLs act in an additive manner. Near-isogenic line plants with the combination of favorable alleles from O. rufipogon and Hwaseong exhibited higher LTG than two introgression lines. With regard to coleoptile length, three QTLs observed on chromosomes 1, 3, and 8 were colocalized with QTLs for LTG, suggesting the pleiotropy of the single gene at each locus. According to the results, the introgression of favorable O. rufipogon alleles could hasten the development of rice with high LTG and high coleoptile elongation in japonica cultivars.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Quantitative Trait Loci for Germination and Coleoptile Length under Low-Temperature Condition Using Introgression Lines Derived from an Interspecific Cross in Rice

Akhtamov

Adeva

Shim

et al. 2020

Genes

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“…Genotyping was conducted in 58 F 3 and 17 F 4 lines using 11 polymorphic SSR markers between CR2002 and Hwaseong. PCR was conducted, as described in Shim et al (2019) [47]. PCR products were separated on 3% metaphor agar stained with StaySafe Nucleic Acid Gel Stain (RBC, Taiwan) or 4% polyacrylamide denaturing gel stained with Silver Staining Kit (Bioneer, Korea), respectively.…”

Section: Dna Extraction and Qtl Analysismentioning

confidence: 99%

A RING-Type E3 Ubiquitin Ligase, OsGW2, Controls Chlorophyll Content and Dark-Induced Senescence in Rice

Shim

Kim

Jeon

et al. 2020

IJMS

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Leaf senescence is the final stage of plant development. Many internal and external factors affect the senescence process in rice (Oryza sativa L.). In this study, we identified qCC2, a major quantitative trait locus (QTL) for chlorophyll content using a population derived from an interspecific cross between O. sativa (cv. Hwaseong) and Oryza grandiglumis. The O. grandiglumis allele at qCC2 increased chlorophyll content and delayed senescence. GW2 encoding E3 ubiquitin ligase in the qCC2 region was selected as a candidate for qCC2. To determine if GW2 is allelic to qCC2, a gw2-knockout mutant (gw2-ko) was examined using a dark-induced senescence assay. gw2-ko showed delayed leaf senescence in the dark with down-regulated expression of senescence-associated genes (SAGs) and chlorophyll degradation genes (CDGs). The association of the GW2 genotype with the delayed senescence phenotype was confirmed in an F2 population. RNA-seq analysis was conducted to investigate 30-day-old leaf transcriptome dynamics in Hwaseong and a backcross inbred line—CR2002—under dark treatment. This resulted in the identification of genes involved in phytohormone signaling and associated with senescence. These results suggested that transcriptional regulation was associated with delayed senescence in CR2002, and RING-type E3 ubiquitin ligase GW2 was a positive regulator of leaf senescence in rice.

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“…(2010) mapped a major QTL ( qCtss11 ) for CTSS from O. rufipogon accession W1943. So far many QTLs (Cen et al., 2018; Li et al., 2019; Liang et al., 2018; Luo et al., 2016; Shim et al., 2019, 2020; Yu et al., 2018; Yuan et al., 2020) and micro‐RNAs (Jiang et al., 2019) conferring cold tolerance have been identified from this species. Luo et al.…”

Section: Introductionmentioning

confidence: 99%

Deployment of wild relatives for genetic improvement in rice (Oryza sativa)

et al. 2020

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Rice is one of the most important crops in the world. The wild species of rice are expected to have novel beneficial alleles that have been lost from cultivated rice during the process of domestication. Therefore, wild species could be the potential source to induce lost genetic diversity in cultivated rice. Serving as an important reservoir of novel genes/QTLs, wild species, in general, are better adapted to different ecologies and can tolerate many biotic and abiotic stresses. Despite that, only a few wild species are studied and extensive characterization both at the molecular and morphological level is yet to be achieved. Several agronomically important genes/QTLs for improving biotic and abiotic stresses, resistance, productivity and grain quality traits were identified from AA genome donor wild species and were tagged with breeder friendly molecular markers for their transfer to elite genetic backgrounds. The present review provides information on the important wild rice species harbouring genes/QTLs for agriculturally important traits and their successful utilization in rice breeding programmes.

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Fine Mapping of a Low-Temperature Germinability QTLqLTG1Using Introgression Lines Derived fromOryza rufipogon

Cited by 17 publications

References 28 publications

Characterization of Quantitative Trait Loci for Germination and Coleoptile Length under Low-Temperature Condition Using Introgression Lines Derived from an Interspecific Cross in Rice

Characterization of Quantitative Trait Loci for Germination and Coleoptile Length under Low-Temperature Condition Using Introgression Lines Derived from an Interspecific Cross in Rice

A RING-Type E3 Ubiquitin Ligase, OsGW2, Controls Chlorophyll Content and Dark-Induced Senescence in Rice

Deployment of wild relatives for genetic improvement in rice (Oryza sativa)

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