Fine mapping of a Phytophthora -resistance locus RpsGZ in soybean using genotyping-by-sequencing

Abstract

Background: Phytophthora root rot (PRR) caused by Phytophthora sojae ( P. sojae ), is one of the most serious limitation to soybean-production in the world. Identification of resistant gene(s) and incorporating them into elite varieties are an effective way for breeding to prevent soybean from being harmed by this disease. A valuable mapping population of 228 F 8:11 recombinant inbred lines (RILs) derived from a cross of resistant cultivar Guizao1 and susceptible cultivar BRSMG68 and a high-density genetic linkage map with an average distance of 0.81 centimorgan (cM) between adjacent bin markers in this population were used to map and explore the candidate gene(s).Results: In this study, the PRR resistance in Guizao1 was controlled by a single Mendelian locus, and was fine mapped to a 367.371-kb genomic region on chromosome 3 that harbours 19 genes, including 7 disease resistance (R)-like genes in the reference Willliams 82 genome. Quantitative real-time PCR assays of possible candidate genes revealed that Glyma.03g05300 was likely involved in PRR resistance.Conclusions: These findings of fine mapping of a novel Rps locus will serve as a basis for cloning, transferring of resistant genes and breeding of P. sojae resistant soybean cultivars through marker-assisted selection.

Mining germplasm panels and phenotypic datasets to identify loci for resistance to Phytophthora sojae in soybean

“…sojae , but the rise in pathotype diversity has made utilizing single Rps genes less effective in many regions (Anderson, Walch, & Kurle, 2012; Costamilan et al., 2013; Dorrance et al., 2016; Grau, Dorrance, Russin, & Bond, 2004; Kaitany, Hart, & Safir, 2001; Ryley et al., 1998; Schmitthenner, Hobe, & Bhat, 1994; Stewart, Abeysekara, & Robertson, 2014; Yan & Nelson, 2019). Along with the recently identified Rps genes, RpsGZ and RpsX (Jiang et al., 2020; Zhong, Li, Sun, Duan, & Zhu, 2019), more than 30 Rps genes/alleles have been proposed and mapped to nine chromosomes (Anderson & Buzzell, 1992; Athow & Laviolette, 1982; Athow, Laviolette, & Mueller, 1980; Buzzell & Anderson, 1981, 1992; Dorrance, 2018b; Gordon, St. Martin, & Dorrance, 2006; Kilen, Hartwig, & Keeling, 1974; Lin et al., 2013; Ping et al., 2016; Ploper, Athow, & Laviolette, 1985; Sahoo, Abeysekara, Cianzio, Robertson, & Bhattacharyya, 2017; Sugimoto et al., 2012; Sun et al., 2011, 2014; Wu et al., 2011a; Yu et al., 2010; Zhang et al., 2013a, 2013b; Zhu, Huo, Wang, Huang, & Wu, 2007), with many located on chromosome (Chr) 3 (Supplemental Table S1). Although more than 30 Rps genes/alleles have been identified, only a few, Rps1a , Rps1b , Rps1c , Rps1k , Rps3a , and Rps6 , have been deployed in soybean cultivars (Abney et al., 1997; Dorrance, 2018b; Grau et al., 2004; Slaminko, Bowen, & Hartman, 2010).…”

High‐throughput NGS‐based genotyping and phenotyping: Role in genomics‐assisted breeding for soybean improvement

“…Recently, reduced cost of GBS has made it increasingly more feasible in soybean compared with WGRS as a method of genotyping (Tables 1 and 2; Alipour et al, 2019; Fang et al, 2019; Gutierrez‐Gonzalez et al, 2019; Jiang et al, 2020). GBS has been routinely used in the gene mapping and other studies in soybean (Table 2).…”