Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa

Saitô, Masanori; Kubo, Nakao; Matsumoto, Satoru; Suwabe, Keita; Tsukada, M.; Hirai, Masashi

doi:10.1007/s00122-006-0412-1

Cited by 133 publications

(125 citation statements)

References 33 publications

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“…Amplified fragment length polymorphism (AFLP) analysis was performed as described previously (Tsuro et al 2005). Brassica simple sequence repeat (SSR) markers (Lowe et al 2004, Suwabe et al 2002, Choi et al 2007, Ling et al 2007, Kim et al 2009) and Brassica sequence-tagged site (STS) markers (Kim et al 2006, Saito et al 2006, Choi et al 2007 were also used for the genotyping. The PCR reactions, detection of polymorphisms and construction of a linkage map were performed as described previously (Sakata et al 2006).…”

Section: Linkage and Qtl Analysesmentioning

confidence: 99%

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

et al. 2010

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The Brassica species displays great variation with regard to morphology. However, the genetic mechanisms that underlie this variability are mostly unknown. We generated 188 F 2 plants derived from a cross between a Chinese cabbage and a vegetable turnip, and scored several morphological traits (head formation, leaf lobe, pubescence, and turnip formation) twice (2005 and 2007) in two subsets of 94 individuals. With the exception of pubescence, we did not find any typical discrete segregation of these traits in F 2 plants, suggesting that they are controlled by multiple genes. We constructed a linkage map to conduct quantitative trait locus (QTL) analysis, and detected eight and 14 QTLs in 2007 and 2005, respectively, for head formation, leaf lobe, pubescence, turnip size, and turnip weight. The amplified fragment length polymorphism (AFLP) fragments linked to the QTLs were converted into sequence-tagged site (STS) markers. The QTLs detected here and their linkage markers may provide useful information for the selection of traits during the breeding of Chinese cabbage and turnip cultivars.

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Section: Linkage and Qtl Analysesmentioning

confidence: 99%

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

et al. 2010

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“…Thus, the development of resistant cultivars is currently the most efficient way to control clubroot among Brassica crops. Both qualitative and quantitative clubroot resistances have been identified and analyzed in different cultivated Brassicaceae species (Manzanares-Dauleux et al, 2000a;Suwabe et al, 2003;Hirai et al, 2004;Piao et al, 2004;Rocherieux et al, 2004;Hirai, 2006;Saito et al, 2006;Suwabe et al, 2006). Monogenic or oligogenic conferred clubroot resistance introduced into commercial cultivars leads to complete resistance (incompatible interaction) but is rapidly overcome.…”

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confidence: 99%

Differential Regulation of Root Arginine Catabolism and Polyamine Metabolism in Clubroot-Susceptible and Partially Resistant Arabidopsis Genotypes

et al. 2008

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The hypertrophy and hyperplasia of infected roots into clubs are the intrinsic characteristics of clubroot, one of the economically most important diseases in Brassica crops worldwide. Polyamines, arginine (Arg)-derived metabolites, have long been recognized as cell proliferation and differentiation regulators in plants and consequently are suitable candidates for potential gall development factors. Furthermore, Arg catabolism, through arginase, which is strongly connected to polyamine metabolism, would play an important role in response to wound trauma and pathogen infection. In this study, we exploited the Arabidopsis (Arabidopsis thaliana)-Plasmodiophora brassicae pathosystem to investigate the involvement of polyamine metabolism and Arg catabolism in host responses to the pathogen infection and in partial clubroot resistance mechanisms. We demonstrated at the transcriptional, enzymatic, and metabolic levels that polyamine metabolism and Arg catabolism are induced during the later stages of disease in compatible Arabidopsis-P. brassicae interactions. However, susceptible and partially resistant plants showed strikingly different Arg metabolism signatures. Susceptible plants were characterized by a transient agmatine production, a massive induction of arginase, and a strong accumulation of proline. The potential functions of this marked activation of the arginase pathway in the P. brassicae pathogenicity strategy are discussed. Partially resistant plants showed a continuous agmatine production and a weaker arginase pathway activity than the susceptible genotype. Results suggest that the symptom severity was strongly associated to the differential regulation of root polyamine metabolism and Arg catabolism. Further work using arginase transgenic plants will provide insight into the physiological function of the arginase pathway in partial clubroot resistance.Clubroot, caused by the obligate biotrophic protist Plasmodiophora brassicae Woron., is one of the economically most important diseases of Brassica crops in the world. The life cycle of this soil-borne pathogen can be divided into two phases: a primary phase in which events are confined to the root hairs, and a secondary phase that occurs in the cortex and the stele of the hypocotyl and roots of the infected plants. During the second phase, multinucleate plasmodia cause the hypertrophy (abnormal cell enlargement) and hyperplasia (uncontrolled cell division) of infected roots into characteristic clubs (Ingram and Tommerup, 1972). These symptoms obstruct nutrient and water transport, stunt the growth of the plant, and consequently reduce crop yield and quality. Since the pathogen survives as resting spores for a long period (up to 15 years) in the soil, control of the disease by agricultural practices and/or chemical treatments is difficult and/ or expensive. Thus, the development of resistant cultivars is currently the most efficient way to control clubroot among Brassica crops. Both qualitative and quantitative clubroot resistances have been identified and a...

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“…A detailed map around Crr3 was constructed on the basis of the synteny between B. rapa and Arabidopsis (Saito et al, 2006). It is probable that resistance genes at the loci CRk and Crr3 are clustering each other.…”

Section: Discussionmentioning

confidence: 99%

Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

Matsumoto¹,

Ueno

Aruga

et al. 2012

J. Japan. Soc. Hort. Sci.

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Many clubroot resistant (CR) cultivars of Chinese cabbage (Brassica rapa ssp. pekinensis) have been bred so far, but their usage is limited because the capacity for resistance breaks down with time. This degradation is caused by a pathogenic variation in the causal fungus Plasmodiophora brassicae. We attempted to accumulate 3 CR genes, CRa, CRk, and CRc, through marker-assisted selection. Five doubled haploid CR lines with an individual CR locus were used as breeding materials. The CR lines were crossed with each other. A subsequent selection for resistance was performed using sequence characterized amplified region markers in segregating generations. As a result, 4 homozygous lines for 3 resistance genes and the F 1 hybrids between them were developed. CR pyramiding lines were inoculated with 6 field isolates of P. brassicae. The homozygous lines for 3 CR genes, whether selfed or crossed, exhibited exceedingly high resistance against all of the isolates. Morphological characters of F 1 hybrids were comparable to those of a control cultivar, but the degree of heterosis was less than expected, which is probably because of genetic similarity of the parents. The results of this study prove that clubroot resistance can be reinforced through the accumulation of varied resistance genes in B. rapa.

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Fine mapping of the clubroot resistance gene, Crr3, in Brassica rapa

Cited by 133 publications

References 33 publications

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

Detection of quantitative trait loci controlling morphological traits in Brassica rapa L.

Differential Regulation of Root Arginine Catabolism and Polyamine Metabolism in Clubroot-Susceptible and Partially Resistant Arabidopsis Genotypes

Accumulation of Three Clubroot Resistance Genes through Marker-assisted Selection in Chinese Cabbage (Brassica rapa ssp. pekinensis).

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