Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting stage, using an Oryza rufipogon Griff. introgression line

Cao, Zhenfu; Yao, Li; Tang, Huiwu; Zeng, Bohong; Tang, Xiuying; Long, Qizhang; Wu, Xiao‐Feng; Cai, Yaohui; Yuan, Linfeng; Jian-lin, Wan

doi:10.1007/s00122-020-03539-7

Cited by 35 publications

(27 citation statements)

References 42 publications

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“…For un lled grain, grains were scored for fertility by checking whether a kernel was present or not. Next, the absolute spikelet fertility percentage was calculated using the formula below, and HT was assessed based on absolute fertility at high temperature [21,28].…”

Section: Methodsmentioning

confidence: 99%

QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice

Chen

Wang

Zhang

et al. 2020

Preprint

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Abstract Background: High-temperature stress can cause serious abiotic damage that limits the yield and quality of rice. Heat tolerance during the flowering stage of rice is a key trait that can guarantee a high and stable yield under heat stress. Heat tolerance is a complex trait that is regulated by numerous quantitative trait loci (QTLs); of which, however, few underlying genes have been fine mapped and cloned.Results: In this study, the F2:3 population derived from a cross between Huanghuazhan (HHZ), a heat-tolerant indica cultivar, and 9311, a heat-sensitive indica rice variety, were used to map the QTLs for heat tolerance during the flowering stage of rice. The F2:3 lines were treated under 38 °C from 9:30 am to 3:30 pm continuously for 3 days in a phytotron, and spikelet fertility was assessed. A new major QTL, qHTT8, controlling heat tolerance was located on chromosome 8 using the bulked-segregant analysis (BSA)-seq method. The QTL qHTT8 was determined to be located in the 355500–4520000 bp region, with a size of 0.965 Mb. The candidate region of qHTT8 on chromosome 8 contained 65 predicted genes. Ten putative predicted genes were found to be associated with abiotic stress tolerance. In future studies, the backcross population will be constructed to fine map and validate the effect of qHTT8.Conclusion: The information obtained in this study is useful for the fine mapping and cloning of qHTT8 and breeding heat-tolerant varieties of rice using marker-assisted selection.

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Section: Methodsmentioning

confidence: 99%

QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice

Chen

Wang

Zhang

et al. 2020

Preprint

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“…Based on physiological responses, introgressed QTLs can be used for the development of HS-tolerant rice cultivars [117]. Similarly, fine mapping of the introgressed QTL (qHTB1-1QTL) at the booting phase confers the HS tolerance using ILs in rice [118].…”

Section: Genomics: Helps To Reveal the Stressresponsive Mechanismsmentioning

confidence: 99%

Can omics deliver temperature resilient ready-to-grow crops?

Raza

Tabassum

Kudapa

et al. 2021

Critical Reviews in Biotechnology

141

110

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Plants are extensively well-thought-out as the main source for nourishing natural life on earth. In the natural environment, plants have to face several stresses, mainly heat stress (HS), chilling stress (CS) and freezing stress (FS) due to adverse climate fluctuations. These stresses are considered as a major threat for sustainable agriculture by hindering plant growth and development, causing damage, ultimately leading to yield losses worldwide and counteracting to achieve the goal of "zero hunger" proposed by the Food and Agricultural Organization (FAO) of the United Nations. Notably, this is primarily because of the numerous inequities happening at the cellular, molecular and/or physiological levels, especially during plant developmental stages under temperature stress. Plants counter to temperature stress via a complex phenomenon including variations at different developmental stages that comprise modifications in physiological and biochemical processes, gene expression and differences in the levels of metabolites and proteins. During the last decade, omics approaches have revolutionized how plant biologists explore stress-responsive mechanisms and pathways, driven by current scientific developments. However, investigations are still required to explore numerous features of temperature stress responses in plants to create a complete idea in the arena of stress signaling. Therefore, this review highlights the recent advances in the utilization of omics approaches to understand stress adaptation and tolerance mechanisms. Additionally, how to overcome persisting knowledge gaps. Shortly, the combination of integrated omics, genome editing, and speed breeding can revolutionize modern agricultural production to feed millions worldwide in order to accomplish the goal of "zero hunger." ARTICLE HISTORY

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“…Zhou et al (2019), by using a hybrid sequencing technology, discovered novel high nighttime temperature stress linked SNPs. Cao et al (2020) fine mapped a heat tolerance conferring QTL from a wild rice accession and identified two heat-stress induced genes. All these significant advances, along with results presented in this study, could greatly accelerate applied research activities for screening and improvement of heat-tolerance in rice germplasm.…”

Section: Recent Advancements In Improving Reproductive Stage Heat-tolmentioning

confidence: 99%

Reproductive tissues-specific meta-QTLs and candidate genes for development of heat-tolerant rice cultivars

Raza

Riaz

Sabar

2020

Preprint

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Rice holds the key to future food security. In rice-growing areas, temperature has already reached an optimum level for growth, hence, any further increase due to global climate change could significantly reduce rice yield. Several mapping studies have identified a plethora of reproductive tissue-specific and heat stress associated inconsistent quantitative trait loci (QTL), which could be exploited for improvement of heat tolerance. In this study, we performed a meta-analysis on previously reported QTLs and identified 35 most consistent meta-QTLs (MQTLs) across diverse genetic backgrounds and environments. Genetic and physical intervals of nearly 66% MQTLs were narrower than 5 cM and 2 Mb respectively, indicating hotspot genomic regions for heat tolerance. Comparative analyses of MQTLs underlying genes with microarray and RNA-seq based transcriptomic data sets revealed a core set of 45 heat-responsive genes, among which 24 were reproductive tissue-specific and have not been studied in detail before. Remarkably, all these genes corresponded to various stress associated functions, ranging from abiotic stress sensing to regulating plant stress responses, and included heat-shock genes (OsBiP2, OsMed37_1), transcription factors (OsNAS3, OsTEF1, OsWRKY10, OsWRKY21), transmembrane transporters (OsAAP7A, OsAMT2;1), sugar metabolizing (OsSUS4, α -Gal III) and abiotic stress (OsRCI2-7, SRWD1) genes. Functional data evidences from Arabidopsis heat-shock genes also suggest that OsBIP2 may be associated with thermotolerance of pollen tubes under heat stress conditions. Furthermore, promoters of identified genes were enriched with heat, dehydration, pollen and sugar responsive cis-acting regulatory elements, proposing a common regulatory mechanism might exist in rice for mitigating reproductive stage heat stress. These findings strongly support our results and provide new candidate genes for fast-track development of heat-tolerant rice cultivars. Key MessageBy integrating genetics and genomics data, reproductive tissues-specific and heat stress responsive 35 meta-QTLs and 45 candidate genes were identified, which could be exploited through marker-assisted breeding for fast-track development of heat-tolerant rice cultivars.

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Fine mapping of the qHTB1-1QTL, which confers heat tolerance at the booting stage, using an Oryza rufipogon Griff. introgression line

Cited by 35 publications

References 42 publications

QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice

QTL Mapping and Identification of Candidate Genes for Heat Tolerance at the Flowering Stage in Rice

Can omics deliver temperature resilient ready-to-grow crops?

Reproductive tissues-specific meta-QTLs and candidate genes for development of heat-tolerant rice cultivars

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