Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538

Abstract

The discovery of biotype diversity of soybean aphid (SA: Aphis glycines Matsumura) in North America emphasizes the necessity to identify new aphid-resistance genes. The soybean [Glycine max (L.) Merr.] plant intro-

Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324

“…The positions of the two QTL on chromosome 13 were coincident to those of resistance genes previously detected in other aphid-resistant PIs. QTL_13_1 was very close to the Rag2 gene (Mian et al, 2008a;Kim et al, 2010), and QTL_13_2 was close to the rag4 gene for aphid resistance in PI 567541B (Zhang et al, 2009). However, Mian et al (2008b) reported that PI 567324 was moderately resistant to aphid biotypes 1 and 2, and Zhang et al (2009Zhang et al ( , 2010 showed that the gene near Satt649 might provide resistance to an unknown aphid biotype that is unlike biotypes 1 and 2.…”

“…Identification of soybean varieties and plant introductions (PIs) that show resistance to soybean aphids and characterization of the genes from the resistant sources are important early steps in the development of cultivars with resistance to soybean aphid. At least four soybean aphid resistance genes (Rag1, Rag2, Rag3 and rag4) have been reported in different genomic regions on soybean chromosomes 7, 13 and 16 (Li et al, 2007;Mian et al, 2008a;Zhang et al, 2009;Kim et al, 2010;Zhang et al, 2010). Soybean cultivars with a single-aphid resistance gene (for example, Rag1 and Rag2) have been developed, and both genetically modified organism (GMO) and non-GMO cultivars with the Rag1 gene are commercially available (Wiarda et al, 2012).…”

“…The Rag2 gene controlling aphid resistance in PI 243540 was mapped to chromosome 13 and the aphid resistance gene in PI 200538 was also located close to the Rag2 locus, although it is not known whether these two aphid resistance genes are identical (Mian et al, 2008a;Hill et al, 2009;Kim et al, 2010). Furthermore, Jun et al (2012) reported a major gene-controlling aphid resistance in PI 567301B, which was also mapped to a position near the Rag2 gene.…”

“…Furthermore, Jun et al (2012) reported a major gene-controlling aphid resistance in PI 567301B, which was also mapped to a position near the Rag2 gene. Recently, Kim et al (2010) conducted fine mapping of the aphid resistance gene in PI 200538 to a 54-kb interval, and the fine mapping of the Rag2 gene in PI 243540 and the resistance gene in PI 567301B are currently in progress (unpublished data). Identification of the exact location for each resistance gene can provide information useful for determining genetic relationships.…”

Mapping an aphid resistance gene in soybean [Glycine max (L.) Merr.] P746

“…Interestingly, PI 200538 was considered to be an additional source of Rag2 because the resistance locus in PI 200538 was located in the same region as Rag2, with both genes exhibiting very similar resistant abilities (Hill et al, 2009). In 2010, Rag2 was mapped into a 54-kb region in PI 200538 (Kim et al, 2010b). Another important antixenosis resistance gene, Rag3 in PI 567543C, was found to be chromosome 16 (LG J) (Mensah et al, 2005;Zhang et al, 2010).…”