Fine mapping of the three rRNA operons on the updated genomic map of Campylobacter jejuni TGH9011 (ATCC 43431)

Kim, N W; Lombardi, Raffaella; Bingham, Hermine L.; Hani, Eric Kurt; Louie, Helena; Ng, David; Chan, Vivien

doi:10.1128/jb.175.22.7468-7470.1993

Cited by 35 publications

(17 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Southern hybridizations were performed to determine the presence of the relevant gene in Campylobacter strains. The hippuricase gene has already been localized (16) on the physical map of C. jejuni TGH9011 (18) and was shown to be present in single copy (data not shown). The type strain of C. jejuni contains a 19-kb ClaI genomic DNA band and a 2.1-kb HindIII genomic DNA band which hybridize to the hippuricase probe ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

Hani

Chan

1995

J Bacteriol

Self Cite

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

Hani

Chan

1995

J Bacteriol

Self Cite

View full text Add to dashboard Cite

“…Unlike the characteristic continuous 16S-23S (eu)bacterial rRNA operon structure seen in E. coli (35), several studies have suggested that in at least one of the three C. jejuni rRNA operons, the 16S and 23S genes are located separately (23,27,37,57). However, recent fine mapping of the three rRNA gene loci of one of these C. jejuni strains has shown that all three operons possess a contiguous 16S-23S structure (25). The sequence and organization of one of these contiguous rRNA operons has recently been described (GenBank accession number Z29326).…”

mentioning

confidence: 93%

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

et al. 1994

Self Cite

View full text Add to dashboard Cite

The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. coli numbering) in the form of an extra sequence block approximately 147 bp long. PCR analysis of 31 other strains of C. coli and C. jejuni showed that 69%o carried a transcribed spacer of either ca. 147 or ca. 37 bp.Comparison of all sequenced Campylobacter transcribed spacers showed that the Campylobacter inserts were related in sequence and percent G+C content. All Campylobacter strains carrying transcribed spacers in their 23S rRNA genes produced fragmented 23S rRNAs. Other strains which produced unfragmented 23S rRNAs did not appear to carry transcribed spacers at this position in their 23S rRNA genes. At the 1850 region (E. coli numbering), Campylobacter 23S rRNA displayed a base pairing signature most like that of the beta and gamma subdivisions of the class Proteobacteria, but in the 270 region, Campylobacter 23S rRNA displayed a helix signature which distinguished it from the alpha, beta, and gamma subdivisions. Phylogenetic analysis comparing C. coli VC167 23S rRNA and a C.jejuni TGH9011 (ATCC 43431) 23S rRNA with 53 other completely sequenced (eu)bacterial 23S rRNAs showed that the two campylobacters form a sister group to the alpha, beta, and gamma proteobacterial 23S rRNAs, a positioning consistent with the idea that the genus Campylobacter belongs to the epsilon subdivision of the class Proteobacteria.The members of the Campylobacter group of organisms are important pathogens in human and veterinary medicine. This group includes Campylobacter, Arcobacter, Helicobacter, Wolinella, and "Flexispira" spp. and has been argued to constitute a separate bacterial lineage within the class Proteobacteria (62, 63). The most recently published phylogenetic tree based on small-subunit rRNA sequences has the group positioned in the delta and epsilon subdivisions of the Proteobacteria (purple bacteria) (39). There are 11 Campylobacter species (9, 56), and the roles of several of these species in diseases of humans and animals have been well described (43). Typically, these small, nutritionally fastidious, microaerophilic, gram-negative spiral bacteria colonize moist body surfaces, including the human gingival cavity, the small intestinal mucosa, and the vaginal mucosa of bovines and this colonization leads to disease. The two most important human pathogens in the genus are Campylobacterjejuni and C. coli, which cause enteritis.In keeping with their small size and limited nutritional properties, the campylobacters have a small genome (23,37,57). At 1.7 to 1.8 Mb, the C. jejuni and C. coli genomes are only 36% of the size of the Escherichia coli chromosome (53) (23,24,27,37,57). Unlike the characteristic continuous 16S-23S (eu)bacterial rRNA operon structure seen in E. coli (35), several studies have sug...

show abstract

“…This allowed us to identify strain-specific diversity which might be involved in virulence potential. These two C. jejuni strains differ by several phenotypic and genotypic features: (i) their Penner serotype: ATCC 43431 (TGH 9011) is Penner serotype O:3, while NCTC 11168 is Penner serotype O:2 (49); (ii) their invasion ability: NCTC 11168 is poorly invasive (3), while ATCC 43431 appears to be highly invasive (17,18); (iii) their genome size: the genome of ATCC 43431 appears to be larger than that of NCTC 11168 (21,34); and (iv) their gene order: gene positions seem to differ considerably between the two strains (21,22,34). Since invasion ability has been shown to correlate with epidemiological outcomes (10), the genes unique to ATCC 43431 will provide a valuable resource to dissect the C. jejuni features that affect pathogenic potential.…”

mentioning

confidence: 99%

Identification ofCampylobacter jejuniATCC 43431-Specific Genes by Whole Microbial Genome Comparisons

2004

View full text Add to dashboard Cite

This study describes a novel approach to identify unique genomic DNA sequences from the unsequenced strain C. jejuni ATCC 43431 by comparison with the sequenced strain C. jejuni NCTC 11168. A shotgun DNA microarray was constructed by arraying 9,600 individual DNA fragments from a C. jejuni ATCC 43431 genomic library onto a glass slide. DNA fragments unique to C. jejuni ATCC 43431 were identified by competitive hybridization to the array with genomic DNA of C. jejuni NCTC 11168. The plasmids containing unique DNA fragments were sequenced, allowing the identification of up to 130 complete and incomplete genes. Potential biological roles were assigned to 66% of the unique open reading frames. The mean G؉C content of these unique genes (26%) differs significantly from the G؉C content of the entire C. jejuni genome (30.6%). This suggests that they may have been acquired through horizontal gene transfer from an organism with a G؉C content lower than that of C. jejuni. Because the two C. jejuni strains differ by Penner serotype, a large proportion of the unique ATCC 43431 genes encode proteins involved in lipooligosaccharide and capsular biosynthesis, as expected. Several unique open reading frames encode enzymes which may contribute to genetic variability, i.e., restriction-modification systems and integrases. Interestingly, many of the unique C. jejuni ATCC 43431 genes show identity with a possible pathogenicity island from Helicobacter hepaticus and components of a potential type IV secretion system. In conclusion, this study provides a valuable resource to further investigate Campylobacter diversity and pathogenesis.

show abstract

Fine mapping of the three rRNA operons on the updated genomic map of Campylobacter jejuni TGH9011 (ATCC 43431)

Cited by 35 publications

References 15 publications

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

Expression and characterization of Campylobacter jejuni benzoylglycine amidohydrolase (Hippuricase) gene in Escherichia coli

Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp

Identification ofCampylobacter jejuniATCC 43431-Specific Genes by Whole Microbial Genome Comparisons

Contact Info

Product

Resources

About