Fine mapping QSc.VR4, an effective and stable scald resistance locus in barley (Hordeum vulgare L.), to a 0.38-Mb region enriched with LRR-RLK and GLP genes

Wang, Yonggang; Xu, Yi; Gupta, Sanjiv; Zhou, Yi; Wallwork, H.; Zhou, Gaofeng; Broughton, Sue; Zhang, Xiaoqi; Tan, Cong; Westcott, Sharon; Moody, D. Branch; Sun, Dongfa; Loughman, Robert; Zhang, Wenying; Li, Chengdao

doi:10.1007/s00122-020-03599-9

Cited by 4 publications

(4 citation statements)

References 140 publications

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“…Leucine-rich repeat motifs consist of 20-30 amino acid residues and differences in the number of LRR motifs, the composition of the motifs and their arrangement within the LRR-RLK appear to provide the structural framework for differential recognition. In barley, several RLK have been implicated in disease resistance, including one associated with scald resistance (Wang et al 2020).…”

Section: Discussionmentioning

confidence: 99%

An Island of Receptor-Like Genes at the Rrs13 Locus on Barley Chromosome 6HS Co-locate with Three Novel Sources of Scald Resistance

Eckstein,

Griffith,

Zhang

et al. 2024

Preprint

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Three Hordeum spontaneum-derived resistances (referred to as 145L2, 41T1 and 40Y5) have demonstrated long-term effectiveness against barley scald, caused by Rhynchosporium commune, in western Canada. Genetic mapping of these resistances in three populations, and the use of five barley genome assemblies, revealed they co-located to a narrowly defined 0.58-1.2 Mbp region of chromosome 6HS containing the Rrs13 scald resistance gene. Differential disease reactions among the three resistances and a Rrs13 carrier to a panel of 24 scald isolates indicated that the four resistances were unique from one another. A marker created to target the 6HS scald locus was screened across a panel of barley germplasm that included H. vulgare, H. spontaneum and H. bulbosum lines. The marker showed specificity to H. vulgare lines known to carry the 6HS scald resistances and to two H. spontaneum lines that trace their origins to Jordan. Within the 0.58-1.2 Mbp region were 2-7 tandemly repeated Leucine-Rich Repeat Receptor-Like Proteins (LRR-RLP) and one Lectin Receptor-Like Kinase (Lec-RLK) genes with abundant sequence variation between them. The well-defined role that RLP and RLK genes play in plant defense responses make them logical candidate resistance genes, with one possible hypothesis being that each unique scald resistance may be encoded by a different RLP that interacts with a common RLK. It is suggested the three scald resistances be temporarily named Rrs13145L2, Rrs1341T1 and Rrs1340Y5to recognize their co-location to the Rrs13 locus until it is determined if these resistances represent unique genes or alleles of the same gene.

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Section: Discussionmentioning

confidence: 99%

An Island of Receptor-Like Genes at the Rrs13 Locus on Barley Chromosome 6HS Co-locate with Three Novel Sources of Scald Resistance

Eckstein,

Griffith,

Zhang

et al. 2024

Preprint

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“…Another putative candidate gene for rhynchosporium resistance within QTL-3H-1 is a germin-like protein (GLP). GLPs were previously found within another rhynchosporium resistance QTL [13]. GLPs were shown to be involved in basal host resistance against several fungal pathogens of cereals in barley [59,60], wheat [60] and rice [61].…”

Section: Genome-wide Association Studiesmentioning

confidence: 97%

“…As R. commune is known to colonise the plant extracellular space, R genes recognising its effectors, or their effect on the plant, are likely to be cell surface-localised RLKs or receptor-like proteins (RLPs) [2,53,54]. Therefore RLKs have been proposed as candidate genes for Rrs1, Rrs18 and QSc.VR4 [9,10,13]. Previously, RLPs and RLKs were shown to be responsible for tomato Cf-2, Cf-4 and Cf-9 resistance against C. fulvum [55], oilseed rape resistance against L. maculans [56], wheat Stb6 and Stb16q resistance against Zymoseptoria tritici [54,57], and rice OsWAK1 resistance against Magnaporthe oryzae [58].…”

Section: Genome-wide Association Studiesmentioning

confidence: 99%

“…In contrast, Rrs2 has been fine mapped to a 0.08 cm interval containing a cluster of Pectin Esterase Inhibitor genes, but none of them individually trigger the Rrs2 resistance response [11,12]. A receptor kinase-like protein has also been suggested as a candidate gene for a quantitative trait locus (QTL) QSc.VR4 [13]. In total, 11 major resistance genes (Rrs genes) have been described, but numerous other QTL have been identified and a full review was conducted in 2020 [14].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm

Thauvin

Russell

Vequaud³

et al. 2022

Agronomy

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Rhynchosporium is one of the main biotic stresses on barley production worldwide. A set of 312 spring barley accessions was tested in four different locations over 3 years, to identify novel genetic resistances to rhynchosporium and to explore the allelic diversity for resistance genes present in this global germplasm collection. High-density genotypes from exome capture and RNA-seq were used to conduct high-resolution association mapping. Seven quantitative trait loci (QTL) were detected, including one in the Rrs2 region, amongst five containing known resistances. Relatively short physical intervals harbouring these resistances were proposed, providing a platform for the identification of underlying genes and tightly linked genetic markers for use in marker assisted selection. Genes encoding kinases were present in four of the QTL, in addition to Rrs1 and Rrs18, two loci known to contribute to rhynchosporium resistance. The frequencies and distributions of these novel and known QTL were superimposed on the regional origin of the landrace genotypes comprising the genome-wide association studies (GWAS) panel, highlighting the value of genetic resources as a source of diverse genetically controlled resistance to rhynchosporium. The detected QTL along with their linked genetic markers, could be exploited either directly for breeding purposes or for candidate gene identification in future studies.

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Anther Culture Protocols for Barley and Wheat

Broughton,

Castello,

Liu

et al. 2024

Plant Cell Culture Protocols

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Fine mapping QSc.VR4, an effective and stable scald resistance locus in barley (Hordeum vulgare L.), to a 0.38-Mb region enriched with LRR-RLK and GLP genes

Cited by 4 publications

References 140 publications

An Island of Receptor-Like Genes at the Rrs13 Locus on Barley Chromosome 6HS Co-locate with Three Novel Sources of Scald Resistance

An Island of Receptor-Like Genes at the Rrs13 Locus on Barley Chromosome 6HS Co-locate with Three Novel Sources of Scald Resistance

Genome-Wide Association Study for Resistance to Rhynchosporium in a Diverse Collection of Spring Barley Germplasm

Anther Culture Protocols for Barley and Wheat

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