Fine needle aspiration biopsy diagnosis of dedifferentiated liposarcoma: Cytomorphology and MDM2 amplification by FISH

Al-Maghraby, Hatem Q.; Khalbuss, Walid E.; Rao, Uma; Cieply, Kathleen; Đačić, Sanja

doi:10.4103/1742-6413.62257

Cited by 18 publications

(13 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MDM2 FISH also is used to provide support for the diagnosis of dedifferentiated liposarcoma when the well differentiated component is not sampled. Recently, the feasibility of using MDM2 FISH in FNA smears was demonstrated for the diagnosis of dedifferentiated liposarcoma 66…”

Section: Introductionmentioning

confidence: 99%

Role of ancillary studies in fine‐needle aspiration from selected tumors

Schmitt

Barroca

2011

Cancer Cytopathology

View full text Add to dashboard Cite

The routine use of ancillary studies is reshaping the practice of cytopathology. Currently, most cytopathologists recognize the importance of immunocytochemistry and molecular techniques as adjuncts to morphology to achieve a precise diagnosis. Cytopathologists also are expected to include specific prognostic and predictive information in their reports. The objective of this review was to address the use of immunocytochemistry and molecular techniques to refine the preoperative diagnosis and classification of lung cancer, thyroid cancer, kidney cancer, gastrointestinal cancer, and soft tissue tumors. Fine-needle aspiration also offers a suitable alternative to biopsy in a variety of clinical settings, in particular, when it may be useful to obtain material to study prognostic and predictive markers. This is particularly relevant to obtain material from metastatic sites. The study of KRAS in colon cancer, CKIT in gastrointestinal stromal tumors, and epidermal growth factor receptor mutational status in lung cancer also are addressed particularly in this report.

show abstract

Section: Introductionmentioning

confidence: 99%

Role of ancillary studies in fine‐needle aspiration from selected tumors

Schmitt

Barroca

2011

Cancer Cytopathology

View full text Add to dashboard Cite

show abstract

“…Well-differentiated liposarcoma (WDLS), which is the mostcommonsubtypeofliposarcoma,canproducemetastases with bone destruction [1]. I read with great interest the articlebyMystakidouandcolleagues [2].IwouldliketocompletethediscussionofMystakidouandcolleaguesbyintroducing a major route by which zoledronic acid could influence bonemetastasesofliposarcoma.…”

Section: Deareditormentioning

confidence: 98%

“…Recent histopathologic findings revealed areas with sheets of osteoclast-type giant cells in the metastatic foci of WDLS [1]. Fusion of the cell membrane of preosteoclasts is anessentialinitialstepinosteoclastmaturation.Cholesterol in the membranes of preosteoclasts has a major role in the osteoclast-like cell formation via cellular membrane fusion…”

Section: Deareditormentioning

confidence: 99%

Well-Differentiated Hand Liposarcoma with Bone Metastases Treated Successfully with Zoledronic Acid: A Molecular Mechanism

Namazi

2012

Onkologie

View full text Add to dashboard Cite

“…Whereas most reported DDLPS cases were recognized in light of a concurrent or prior history of WDLPS, in approximately half of the cases of PLPS the authors initially favored other diagnostic possibilities, including poorly differentiated carcinoma and high-grade sarcoma without specifying the line of differentiation. [24][25][26][27] In this study, we analyze the cytologic aspirates of 38 histologically confirmed high-grade liposarcomas, including 25 DDLPS and 13 PLPS, correlating the cytomorphology with clinical, histologic, immunohistochemical, and genetic data.…”

Section: Introductionmentioning

confidence: 99%

Dedifferentiated liposarcoma and pleomorphic liposarcoma

Mariño-Enríquez

Hornick

Cin

et al. 2013

Cancer Cytopathology

View full text Add to dashboard Cite

BACKGROUND: Dedifferentiated liposarcoma (DDLPS) and pleomorphic liposarcoma (PLPS) are distinct high-grade liposarcomas. DDLPS is a nonlipogenic sarcoma characterized by amplification of MDM2 and CDK4. PLPS is a high-grade sarcoma containing lipoblasts, characterized by a complex karyotype and a more aggressive clinical course. Rarely, DDLPS shows lipogenic differentiation, mimicking PLPS. The cytomorphologic features of DDLPS and PLPS and the utility of ancillary studies have not been systemically analyzed. METHODS: Cytologic preparations of 25 DDLPS and 13 PLPS, all histologically confirmed, were retrospectively reviewed along with clinical and cytogenetic data. Sample cellularity, vascular architecture, background material, predominant cell morphology, quality of the cytoplasm, and nuclear pleomorphism were compared for both tumor types. Immunohistochemistry for MDM2 and CDK4 was performed on cell blocks and/or core needle biopsies. RESULTS: Fine-needle aspirate smears from both DDLPS and PLPS were variably cellular, composed of cellular clusters and noncohesive cells. Abundant myxoid stroma was present in 25% of DDLPS and PLPS cases, whereas branching curvilinear vessels were more common in DDLPS than in PLPS (7 of 25 versus 2 of 13). Tumors were composed of predominantly spindled (18 of 25 DDLPS versus 3 of 13 PLPS) or epithelioid cells (7 of 25 DDLPS versus 6 of 13 PLPS). Pleomorphic cells were predominant in 3 PLPS, and were frequent in both (13 of 25 DDLPS versus 10 of 13 PLPS). The cytoplasm was mostly fibrillary and often vacuolated in both entities. Other features included necrosis, mitoses, and a prominent inflammatory infiltrate. The main cytologic differences were the presence of marked pleomorphism, abundant lipoblasts, and cells with microvacuolated cytoplasm in most PLPS. A total of 24 (96%) and 20 (80%) cases of DDLPS expressed MDM2 and CDK4, respectively, whereas none of the PLPS expressed both markers. Six DDLPS tested showed ring or giant marker chromosomes and/or MDM2 amplification by fluorescence in situ hybridization; 2 PLPS had complex karyotypes. CONCLUSIONS: DDLPS and PLPS exhibit variable and occasionally overlapping cytologic features. Original Article INTRODUCTIONLiposarcomas are a heterogeneous group of malignant adipocytic neoplasms that consist of 3 distinct clinicopathological entities: well-differentiated/dedifferentiated liposarcoma, myxoid liposarcoma, and pleomorphic liposarcoma (PLPS). Collectively, liposarcomas account for approximately 20% of all adult sarcomas. 1 Dedifferentiated liposarcoma (DDLPS) results from the progression of a well-differentiated liposarcoma (WDLPS) to a nonlipogenic sarcoma of variable histologic grades and morphologic patterns that acquires metastatic potential. 2,3 Dedifferentiated areas in DDLPS have the appearance of a high-grade sarcoma in approximately 90% of cases, and show a variety of growth patterns including, most frequently, nondistinct spindle cell and pleomorphic patterns, and less commonly, inflammatory, giant cell, round cell, or...

show abstract

Fine needle aspiration biopsy diagnosis of dedifferentiated liposarcoma: Cytomorphology and MDM2 amplification by FISH

Cited by 18 publications

References 23 publications

Role of ancillary studies in fine‐needle aspiration from selected tumors

Role of ancillary studies in fine‐needle aspiration from selected tumors

Well-Differentiated Hand Liposarcoma with Bone Metastases Treated Successfully with Zoledronic Acid: A Molecular Mechanism

Dedifferentiated liposarcoma and pleomorphic liposarcoma

Contact Info

Product

Resources

About