Fine needle aspiration cytology and flow cytometry immunophenotyping of non‐Hodgkin lymphoma: can we do better?

Zeppa, Pio; Vigliar, Elena; Cozzolino, I; Troncone, Giancarlo; Picardi, Marco; Renzo, Amalia De; Grimaldi, F.; Pane, Fabrizio; Vetrani, A; Palombini, Lucio

doi:10.1111/j.1365-2303.2009.00725.x

Cited by 74 publications

(95 citation statements)

References 35 publications

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“…Furthermore, we were able to perform cytogenetic analysis for MYC, IGH-BCL-2, and BCL-6 rearrangement in all our DLBCL cases to identify aggressive subtypes (presence of IGH-MYC, MYC rearrangement, or concomitant presence of MYC and IGH-BCL-2 and/or BCL-6 rearrangements) (33,34). We also demonstrate subclassification of low-grade NHL, using a combination of immunohistochemistry on cell block and flow cytometry into CLL-SLL, follicular lymphoma, mantle cell lymphoma, and marginal zone lymphoma (35)(36)(37). Moreover, adequate material was available after this multimodality testing approach for IGH-TCR gene rearrangement studies by polymerase chain reaction, when this was required.…”

Section: Discussionmentioning

confidence: 80%

Diagnosis and Subtyping of De Novo and Relapsed Mediastinal Lymphomas by Endobronchial Ultrasound Needle Aspiration

Moonim¹,

Breen²,

Fields

et al. 2013

Am J Respir Crit Care Med

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Rationale: The current management of lymphoma requires accurate diagnosis and subtyping of de novo lymphoma and of relapsed or refractory lymphoma in known cases. The role of endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) in the clinical management of lymphomas is unclear. Objectives: To investigate the use of EBUS-TBNA in the diagnosis of de novo and relapsed mediastinal lymphomas. Methods: A total of 2,256 consecutive patients who underwent EBUS-TBNA in a tertiary center between February 2008 and April 2013 were prospectively evaluated. The diagnostic accuracy and clinical use of EBUS-TBNA in 100 cases of de novo or suspected relapsed mediastinal lymphoma was investigated by comparing EBUS-TBNA diagnosis with the final diagnosis. Measurements and Main Results: De novo mediastinal lymphoma was correctly diagnosed by EBUS-TBNA in 45 (88%) of 51 and relapsed lymphoma in 15 (100%) of 15 lymphoma cases. EBUS-TBNA accurately established a diagnosis other than lymphoma in 32 (97%) of 33 patients with suspected lymphoma relapse. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of EBUS-TBNA in the diagnosis of mediastinal lymphoma were 89%, 97%, 98%, 83%, and 91%, respectively. Sensitivity of EBUS-TBNA in subtyping lymphomas into high-grade non-Hodgkin lymphoma, low-grade non-Hodgkin lymphoma, and Hodgkin lymphoma was 90%, 100%, and 79%, respectively. EBUS-TBNA diagnosis was adequate for clinical management in 84 (84%) of 100 cases. Conclusions: Multimodality evaluation of EBUS-TBNA can be successful in the diagnosis of de novo mediastinal lymphomas and is ideally suited in distinguishing lymphoma relapse from alternative pathologies; it is least sensitive in subtyping Hodgkin lymphoma.

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Section: Discussionmentioning

confidence: 80%

Diagnosis and Subtyping of De Novo and Relapsed Mediastinal Lymphomas by Endobronchial Ultrasound Needle Aspiration

Moonim¹,

Breen²,

Fields

et al. 2013

Am J Respir Crit Care Med

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“…Several studies have demonstrated the usefulness of FC in diagnosing lymphoma by means of fine needle aspiration (FNA) cytology as well as in staging and follow-up (18)(19)(20)(21)(22)(23)(24). However, only few reports are available regarding the role of FC in tissue diagnosis of lymphoma (25)(26)(27)(28)(29)(30)(31), and literature mainly reports tissue FC studies in selected lymphoma subtypes (12,14,(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48).…”

Section: Discussionmentioning

confidence: 99%

Tissue flow cytometry immunophenotyping in the diagnosis and classification of non‐Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases

Demurtas

Stacchini

Aliberti

et al. 2013

Cytometry Part B Clinical

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“…Ravinsky and Morales [40] found that a complete diagnosis could be reached in up to 82% of NHL cases. Recently, Zeppa et al [41], analyzing 446 cases of benign reactive hyperplasia and NHL, demonstrated that FNAC + flow cytometry are effective in the cytological diagnosis and classification of NHL with reproducible results. On the other hand, the exiguity of diagnostic material and clinicopathological limitations (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the exiguity of diagnostic material and clinicopathological limitations (e.g. necrosis or fibrosis in the examined area, partial involvement) can represent an obstacle to further improvements of results [41]. Thus, a biopsy should be preferred, while FNAC could be performed in patients for whom excisional biopsy is contraindicated or for ancillary studies.…”

Section: Discussionmentioning

confidence: 99%

Primary Extranodal Non-Hodgkin’s Lymphoma of the Vagina: A Case Report and a Review of the Literature

Guastafierro

Tedeschi²,

Criscuolo³

et al. 2012

Acta Haematol

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Primary lymphoma of the female genital tract is very rare. We report the case of a 36-year-old woman who was referred to our hospital because of an indeterminate Pap smear test. The colposcopy showed a thickening of the posterior vaginal wall and various irregular ulcerated nodular lesions. Histological examination, immunohistochemistry and the staging procedures were conclusive of diffuse large B-cell lymphoma of the vagina, stage IEA. Complete remission was achieved after 6 cycles of immunopolychemotherapy (rituximab, cyclophosphamide, doxorubicin, vincristine and prednisone). No relapse has occurred during a follow-up of 71 months. Moreover, we reviewed the 62 previously reported cases of primary extranodal non-Hodgkin’s lymphoma of the vagina, focusing on clinicopathological and therapeutic aspects, to better characterize this unusual disease.

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Fine needle aspiration cytology and flow cytometry immunophenotyping of non‐Hodgkin lymphoma: can we do better?

Abstract: FNAC/FC is a fundamental tool in the diagnosis and classification of NHL but the exiguity of diagnostic material and other technical and clinical limitations will probably continue to limit further improvement of the technique.

Cited by 74 publications

References 35 publications

Diagnosis and Subtyping of De Novo and Relapsed Mediastinal Lymphomas by Endobronchial Ultrasound Needle Aspiration

Diagnosis and Subtyping of De Novo and Relapsed Mediastinal Lymphomas by Endobronchial Ultrasound Needle Aspiration

Tissue flow cytometry immunophenotyping in the diagnosis and classification of non‐Hodgkin's lymphomas: A retrospective evaluation of 1,792 cases

Primary Extranodal Non-Hodgkin’s Lymphoma of the Vagina: A Case Report and a Review of the Literature

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