Introduction: Objective Structured Practical Examination (OSPE) is an effective method for formative and summative assessment of undergraduate students during practical examination. Aim: The aim of the study was to study the effectiveness of OSPE in the subject of Pathology and to evaluate them on the basis of these parameters, provide feedback on performance, evaluate on basis of practical skills and measure minimal competences. Materials and methods: This study was conducted among a total of 120 M.B.B.S. students during their second year of MBBS course. OSPE based practical examination was carried out followed by a close ended questionnaire to evaluate student’s perception regarding the same. Results: OSPE was seen as a useful exercise by 83 % of the students. Feedback from the undergraduate students put forward that 17 % students felt that OSPE was an objective tool in evaluating practical skills. Conclusion: OSPE is a more valid and beneficial learning tool according to the students as well as faculty. Keywords: Objective structured practical examination, Pathology, Medical education, Teaching tool