Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Chung, Meng Ching; Tsai, M. H.; Que, Danielle E.; Bongo, Sayre J.; Hsu, Wen Li; Tayo, Lemmuel L.; Lin, Yi Hsien; Lin, Sheng Lun; Gou, Yan; Hsu, Yi Chyun; Hou, Wen Che; Huang, Kuo‐Lin; Chao, How‐Ran

doi:10.4209/aaqr.2019.03.0127

Cited by 28 publications

(25 citation statements)

References 55 publications

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“…Zhao et al, (2014) also found that TRAP PM 2.5 did not induce any adverse effects on the C. elegans after acute and prolonged exposure. One our previous report also showed no lethality for any ambient PM 2.5 sample collected from rural areas including NPUST (0.00144-1440 mg L -1 ) and Linluo Junior High School (0.00115-1150 mg L -1 ) (Chung et al, 2019). Further tests were made in order to confirm that PM 2.5 did not cause immediate mortality to the exposed nematodes.…”

Section: Lethal Effects Of Pm25 To C Elegansmentioning

confidence: 87%

“…Although this result is still unclear, further studies are necessary to identify the underlying mechanism. One our previous study also showed that high ambient PM 2.5 concentration had a negative effect on the brood size of the C. elegans (Chung et al, 2019). Moreover, several studies have demonstrated the dose-dependent manner reduction of the brood size of the nematodes exposed to different toxic compounds, such as PM 2.5 from coal combustion (Sun et al, 2015), cadmium (Wang et al, 2018), zearalenone (Yang et al, 2018), and silver nanomaterial (Moon et al, 2019).…”

Section: Effects Of Trap Pm25 On the Brood Size Of C Elegansmentioning

confidence: 87%

“…The 1 mm long free-living soil nematode used in the animal model Caenorhabditis elegans (C. elegans) established by Sydney Brenner in 1965 plays a key role in the decomposition and nutrient cycling (Sohlenius, 1980). The C. elegans in-vivo system has been used as a model for the assessment of toxic effects of air pollutants (Zhao et al, 2014;Sun et al, 2015Sun et al, , 2016Yang et al, 2016;Wu et al, 2017;Wang et al, 2018;Chung et al, 2019;Zhao et al, 2019) because it shows much advantages over other animal models: production of large number of progeny (100-200 from a single hermaphrodite), transparency, rapid life cycle (3 days), short lifespan (2-3 weeks), low cost, and easy laboratory cultivation (Brenner, 1974;Nass and Hamza, 2007). Moreover, C. elegans follows the widely accepted ethical principles, known as Three Rs (Reduction of the use of higher animals, Refinement of current techniques, and Replacement of animals with alternative methods) (Brenner, 1974).…”

Section: Introductionmentioning

confidence: 99%

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Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model

Chung

Huang

Avelino

et al. 2020

Aerosol Air Qual. Res.

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In association with the mortality rate due to air pollution, vehicular emitted fine particles (PM 2.5) are a threat to public health. PM 2.5-induced in-vivo studies on environmental microorganisms can be used to assess the adverse impacts of PM 2.5 on human health. In the present study, the toxicity of traffic-related-air-pollutant (TRAP) PM 2.5 was evaluated in the animal model Caenorhabditis elegans (C. elegans) using different toxicological endpoints such as lethality, survivability (lifespan), behavioral (head thrashing and body bending), and reproduction (brood size). The TRAP PM 2.5 sample were collected in Taichung City, Taiwan from Mar 24 to April 15 in 2018. Of these 23 day samples, three samples (Days A, B, and C) were randomly selected. The results showed that no immediate lethality was observed after acute (24 h) exposure of the nematodes. On the other hand, sublethal endpoints of reproduction exhibited statistically significant dose-dependent reduction, although Day A and Day C did not decrease the egg-laying capability of the worms. For the neurological toxicity, it is inferred that the higher the PM 2.5 concentrations, the more the adverse effects of neurobehavior (head trashing and body bending) it poses on the C. elegans. The lifespans of nematodes exposed to heavily TRAP PM 2.5 were significantly shortened compared with those of untreated ones based on survival rate. The nematodes exposed PM 2.5 models not only posed potentially adverse health effects on human but also represented ecotoxic impacts on the ecosystem. In conclusion, heavy concentrations of TRAP PM 2.5 significantly and severely disrupted toxicological endpoints of neurology and reproduction to C. elegans. TRAP PM 2.5 significantly shortened the lifespan of the nematodes compared with the control. TRAP PM 2.5 might more severely influenced the specific toxic endpoints, such as lifespan and neurobehavira, in this in-vivo models compared with the reproductive endpoints.

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Section: Lethal Effects Of Pm25 To C Elegansmentioning

confidence: 87%

Section: Effects Of Trap Pm25 On the Brood Size Of C Elegansmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

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Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model

Chung

Huang

Avelino

et al. 2020

Aerosol Air Qual. Res.

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“…Caenorhabditis elegans (C. elegans), a nematode living in soil, has been established as an in vivo model for various applications such as in drug, nanomaterial, and chemical assessments in the biomedical field as well as for studying the toxicity of environmental contaminants such as metals, chemicals, fine particulate matter, and nanomaterials (Chung et al, 2019;Queiros et al, 2019). Its advantages over other in vivo models include a short life cycle and lifespan, easy maintenance, completely sequenced genome, large brood size, and a small, transparent body (Brenner, 1974;Shen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans

Que

Hou

Ang

et al. 2020

Aerosol Air Qual. Res.

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Silica nanoparticles (SiO 2 NPs) are engineered nanomaterials (ENMs) that have a wide range of application. Increased use in manufacturing has led to concerns about their environmental impact and possible adverse health effects. We conducted a comparative toxicity assessment of bare SiO 2-NPs and amine-functionalized SiO 2 NPs (NH 2-SiO 2 NPs) utilizing the Caenorhabditis elegans (C. elegans) in vivo model. L1 nematodes were exposed to exposure concentrations of 0.25, 0.5, 2.5, and 5 mg mL-1 until the worms reached the L4 stage. The chronic lethality and lifespan assays revealed a significant decrease in survival rate and lifespan at 2.5 and 5 mg mL-1 for nematodes exposed to bare SiO 2 NPs (89% and 88%; 22 days, p < 0.05 and 14 days, p < 0.05) and at 5 mg mL-1 for the NH 2-SiO 2 NPs-exposed group (86%; 20 days, p < 0.001). Exposure to all SiO 2 NP concentrations reduced progeny production to 79-60% while exposure to 2.5 and 5 mg mL-1 of NH 2-SiO 2 NPs significantly reduced the brood size to 64-63%. Neurobehavioral toxicity was also observed in the SiO 2 NP-exposed worms, which displayed significantly decreased head thrashing for up to 92-71% and in the NH 2-SiO 2 NPs-exposed worms which showed significantly reduced head thrashing movement for up to 91-85% at concentrations of 0.5-5 mg mL-1. Body bending movements were also significantly reduced at 0.5-5 mg mL-1 SiO 2 NPs (71-34%) and 2.5-5 mg mL-1 NH 2-SiO 2 NPs (94-66%). Significant shortening of body size was also observed in nematodes exposed to 0.5-5 mg mL-1 for both SiO 2 NPs (93-81%) and NH 2-SiO 2 NPs (94-88%). Overall, bare SiO 2 NPs were observed to be more toxic due to the negatively charged surface OH groups, which may have disrupted protein homeostasis, resulting in the observed toxicities. We suggest that functionality is an important indicator in nanosafety evaluations.

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“…Therefore, the combined application of different acellular methods on the same samples is strongly suggested. Although some recent studies were focused on the in vivo evaluation of PM's negative impacts on living organisms [18][19][20], knowledge about the relationships between OP and PM's toxicological effects still presents some important gaps [6,20,21].Other still unresolved issues are related to the assay's design and the influence of the operative conditions by which OP tests are performed. In fact, over recent years, many researchers underlined how test conditions could influence collected data.…”

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confidence: 99%

Application of DPPH Assay for Assessment of Particulate Matter Reducing Properties

et al. 2019

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Different acellular assays were developed to measure particulate matter’s (PM) oxidative potential (OP), a metric used to predict the ability of PM in generating oxidative stress in living organisms. However, there are still fundamental open issues regarding the complex redox equilibria among the involved species which could include reducing compounds. The aim of this study was the pilot application of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay to PM in order to evaluate the presence of reducing species. The assay, commonly applied to biological matrices, was adapted to PM and showed good analytical performances. It allowed the analysis of conventional 24 h airborne PM samples with suitable sensitivity and good repeatability of the measurements. The assay was applied to seven samples representing possible PM contributes (certified urban dust NIST1648a; brake dust; Saharan dust; coke dust; calcitic soil dust; incinerator dust; and diesel particulate matter certified material NIST1650b) and to PM2.5 field filters. The same samples were also analyzed for elements. Preliminary results indicated that the assay gave a linear response and that detectable amounts of reducing species were present in PM samples. The combined application of DPPH and conventional OP assays could then permit, in the future, to gain more knowledge about the reaction and/or competition between oxidative and reducing processes.

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Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Cited by 28 publications

References 55 publications

Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model

Toxic Assessment of Heavily Traffic-related Fine Particulate Matter Using an in-vivo Wild-type Caenorhabditis elegans Model

Toxic Effects of Hydroxyl- and Amine-functionalized Silica Nanoparticles (SiO2 and NH2-SiO2 NPs) on Caenorhabditis elegans

Application of DPPH Assay for Assessment of Particulate Matter Reducing Properties

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