Fine root and root hair morphology of cotton under drought stress revealed with RhizoPot

Xiao, Shuang; Liu, Liantao; Zhang, Yongjiang; Sun, Hong; Zhang, Ke; Bai, Zhiying; Dong, Hezhong; Li, Cundong

doi:10.1111/jac.12429

Cited by 58 publications

(61 citation statements)

References 87 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The experiment consisted of six replicates. RhizoPot (Figure 1), an improvised in situ root device, was used as the culture device (Xiao et al, 2020). The RhizoPot contained 15 kg of a soil mixture.…”

Section: Plant Materials and Experimental Designmentioning

confidence: 99%

“…RhizoPot is assembled from acrylic plates, and a scanning plate with a scanner is placed on an inclined surface to continuously obtain high-definition in situ images of fine roots and root hairs. It is necessary to connect the scanner to a portable computer when collecting images (Xiao et al, 2020). The device enables in situ, accurate, and continuous observation of the root system without destroying the original growth conditions of the root system.…”

Section: Introductionmentioning

confidence: 99%

“…However, the dynamics of morphological changes of cotton roots under P-deficient conditions, especially the response of lateral roots and root hairs to P-deficient stress, are still unclear. In this study, root phenotypic characteristics of two cotton varieties were examined in situ using a root culture device improvised in our laboratory (Xiao et al, 2020). This device achieved higher efficiency, lower cost, easy operation, and high-resolution imaging and can continuously obtain phenotypic images of cotton roots non-destructively under P-deficiency stress.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In situ Root Phenotypes of Cotton Seedlings Under Phosphorus Stress Revealed Through RhizoPot

Zhang

Zhu

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

Phosphorus (P) deficiency is a common challenge in crop production because of its poor mobility through the soil. The root system plays a significant role in P absorption from the soil and is the initial indicator of low P levels. However, the phenotypic dynamics and longevity of cotton roots under P stress remain unknown. In this study, RhizoPot, an improvised in situ root observation device, was used to monitor the dynamics of root phenotypes of cotton seedlings under P-deficient (PD) and P-replete (PR) conditions. Low P stress reduced P absorption and accumulation in the roots, leading to low dry weight accumulation. Cotton seedlings responded to low P stress by increasing the number of lateral roots, specific root length, branch density, root length density, and length of root hairs. Additionally, the life span of root hairs was prolonged. Low P stress also reduced the average diameter of roots, promoted root extension, expanded the root coverage area, and increased the range of P acquisition. Principal component analysis revealed that the net root growth rate, root length density, root dry weight, P absorption efficiency, average root hair length, and taproot daily growth significantly influenced the cotton root architecture. Collectively, these results show that low P stress reduces the net growth rate of cotton seedling roots and restricts plant growth. Plants respond to P deficiency by extending the life span of root hairs and increasing specific root length and lateral root branch density. This change in root system architecture improves the adaptability of plants to low P conditions. The findings of this study may guide the selection of cotton varieties with efficient P utilization.

show abstract

Section: Plant Materials and Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In situ Root Phenotypes of Cotton Seedlings Under Phosphorus Stress Revealed Through RhizoPot

Zhang

Zhu

et al. 2021

Front. Plant Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…While rhizosheath weight was directly correlated with root hair length in wheat (Delhaize et al, 2012), these traits were weakly associated in an array of barley genotypes (George et al, 2014), and no association was observed in diverse chickpea genotypes (Pang et al, 2017). Whereas drying stress increased root hair length of cotton (Xiao et al, 2020) and orange (Zhang et al, 2019), root hair length of both tomato genotypes decreased under drying stress conditions (Figure 1D, Table 1). Nevertheless, the roots of the WT plants held more sand per unit of root hair length as the sand dried (Figure 2B), which may help acquire soil moisture by increasing root-soil contact in sandy soils (North and Nobel, 1997;Smith et al, 2011).…”

Section: Discussionmentioning

confidence: 94%

Abscisic Acid Mediates Drought-Enhanced Rhizosheath Formation in Tomato

et al. 2021

View full text Add to dashboard Cite

The rhizosheath, commonly defined as soil adhering to the root surface, may confer drought tolerance in various crop species by enhancing access to water and nutrients under drying stress conditions. Since the role of phytohormones in establishing this trait remains largely unexplored, we investigated the role of ABA in rhizosheath formation of wild-type (WT) and ABA-deficient (notabilis, not) tomatoes. Both genotypes had similar rhizosheath weight, root length, and root ABA concentration in well-watered soil. Drying stress treatment decreased root length similarly in both genotypes, but substantially increased root ABA concentration and rhizosheath weight of WT plants, indicating an important role for ABA in rhizosheath formation. Neither genotype nor drying stress treatment affected root hair length, but drying stress treatment decreased root hair density of not. Under drying stress conditions, root hair length was positively correlated with rhizosheath weight in both genotypes, while root hair density was positively correlated with rhizosheath weight in well-watered not plants. Root transcriptome analysis revealed that drought stress increased the expression of ABA-responsive transcription factors, such as AP2-like ER TF, alongside other drought-regulatory genes associated with ABA (ABA 8′-hydroxylase and protein phosphatase 2C). Thus, root ABA status modulated the expression of specific gene expression pathways. Taken together, drought-induced rhizosheath enhancement was ABA-dependent, but independent of root hair length.

show abstract

“…Root system configurations such as root hair, root branches and root density can significantly affect the water deficiency of plants. Drought stress can inhibit the development of cotton seedlings, promote the elongation and thinning of fine roots, shorten the life of fine roots with different diameters, promote the elongation of root hairs and accelerate their death [21]. Cunninghamia lanceolate can increase root complexity and elongation, reduce root branching angles, leading to steeper and deeper roots system to adapt to drought stress [22].…”

Section: Drought Stress and The External Form Of Plantsmentioning

confidence: 99%

A Review on Response Mechanism of Plants to Drought Stress

Yang¹,

Lu²,

Wang³

et al. 2021

Preprint

133

View full text Add to dashboard Cite

With the global climate anomalies and the destruction of ecological balance, water shortage has become a serious ecological problem facing all mankind, and drought has become a key factor restricting the development of agricultural production. Therefore, it is essential to study the drought tolerance of crops. On the basis of previous studies, we reviewed the effects of drought stress on plant morphology and physiology, including the changes of external morphology and internal structure of root, stem and leaf, the effects of drought stress on osmotic regulation sub-stances, drought-induced proteins and active oxygen metabolism of plants. In this paper, the main drought stress signals and signal transduction pathways in plants are described, and the functional genes and regulatory genes related to drought stress are listed respectively. We summarize the above aspects in order to provide valuable background knowledge and theoret-ical basis for future agriculture and forestry breeding and cultivation.

show abstract

Fine root and root hair morphology of cotton under drought stress revealed with RhizoPot

Cited by 58 publications

References 87 publications

In situ Root Phenotypes of Cotton Seedlings Under Phosphorus Stress Revealed Through RhizoPot

In situ Root Phenotypes of Cotton Seedlings Under Phosphorus Stress Revealed Through RhizoPot

Abscisic Acid Mediates Drought-Enhanced Rhizosheath Formation in Tomato

A Review on Response Mechanism of Plants to Drought Stress

Contact Info

Product

Resources

About