Fine-scale excitatory cortical circuits reflect embryonic progenitor pools

Ellender, Tommas J.; Avery, Sophie V.; Mahfooz, Kashif; Klemperer, Alexander von; Nixon, Sophie L.; Buchan, Matthew J.; Rheede, Joram J. van; Gatti, A; Waites, Cameron; Newey, Sarah E.; Akerman, Colin J.

doi:10.1101/363069

Cited by 3 publications

(3 citation statements)

References 28 publications

(34 reference statements)

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“…Biases in long-range excitatory synaptic connectivity in the cortex can arise from distinct embryonic progenitor pools ( Ellender et al., 2018 , 2019 ). Next, we explored whether aIP- and OP-derived SPNs differentially sample excitatory input coming from distinct cortical regions.…”

Section: Resultsmentioning

confidence: 99%

Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon

et al. 2021

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“…Previous approaches combining whole-cell patch-clamp recordings with genetic analysis often used RT-PCR and was limited to looking at predetermined subsets of genes [12]. To provide an unbiased assessment of gene expression as well as being able to interrogate the electrical and circuit properties of mature neurons we and other groups have used an approach that combines whole-cell patch-clamp electrophysiology with subsequent single-cell RNA sequencing [1,3,6]. In particular, we used in utero electroporation techniques to fluorescently label cortical neurons derived from distinct progenitor pools and used this approach to characterize their intrinsic and circuit properties as well gene expression [3].…”

Section: Introductionmentioning

confidence: 99%

“…To provide an unbiased assessment of gene expression as well as being able to interrogate the electrical and circuit properties of mature neurons we and other groups have used an approach that combines whole-cell patch-clamp electrophysiology with subsequent single-cell RNA sequencing [1,3,6]. In particular, we used in utero electroporation techniques to fluorescently label cortical neurons derived from distinct progenitor pools and used this approach to characterize their intrinsic and circuit properties as well gene expression [3]. Here we provide a detailed step-by-step instruction on how to progress from making acute brain slices to performing whole-cell patch-clamp recordings under conditions that allow for the successful extraction and processing of the mRNA from the cytoplasm of the patched neuron (Fig.…”

Section: Introductionmentioning

confidence: 99%

Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing

Mahfooz

Ellender

2020

Patch Clamp Electrophysiology

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To understand how the brain functions we need to understand the properties of its constituent cells. Whole-cell patch-clamp recordings of neurons have enabled studies of their intrinsic electrical properties as well as their synaptic connectivity within neural circuits. Recent technological advances have now made it possible to combine this with a sampling of their transcriptional profile. Here we provide a detailed description how to combine whole-cell patch-clamp recordings of neurons in brain slices followed by extraction of their cytoplasm suitable for single-cell RNA-sequencing and analysis.

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Distinct neural progenitor pools in the ventral telencephalon generate diversity in striatal spiny projection neurons

Heusden¹,

Macey-Dare²,

Krajeski³

et al. 2019

Preprint

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Heterogeneous populations of neural progenitors in the embryonic lateral ganglionic eminence (LGE) generate all GABAergic spiny projection neurons (SPNs) found in the striatum. Here we investigate how this diversity in neural progenitors relates to diversity of adult striatal neurons and circuits. Using a combination of in utero electroporation to fluorescently pulse-label striatal neural progenitors in the LGE, brain slice electrophysiology, electrical and optogenetic circuit mapping and immunohistochemistry, we characterise a population of neural progenitors enriched for apical intermediate progenitors (aIPs) and a distinct population of other progenitors (OPs) and their neural offspring. We find that neural progenitor origin has subtle but significant effects on the properties of striatal SPNs. Although aIP and OP progenitors can both generate D1-expressing direct pathway as well as D2-expressing indirect pathway SPNs found intermingled in the striatum, the aIP derived SPNs are found in more medial aspects of the striatum, exhibit more complex dendritic arbors with higher spine density and differentially sample cortical input. Moreover, optogenetic circuit mapping of the aIP derived neurons show that they further integrate within striatal circuits and innervate both local D1 and D2 SPNs. These results show that it is possible to fluorescently pulse-label distinct neural progenitor pools within the LGE and provide the first evidence that neural progenitor heterogeneity can contribute to the diversity of striatal SPNs.

show abstract

Fine-scale excitatory cortical circuits reflect embryonic progenitor pools

Cited by 3 publications

References 28 publications

Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon

Diversity in striatal synaptic circuits arises from distinct embryonic progenitor pools in the ventral telencephalon

Combining Whole-Cell Patch-Clamp Recordings with Single-Cell RNA Sequencing

Distinct neural progenitor pools in the ventral telencephalon generate diversity in striatal spiny projection neurons

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