Fine specificity of a continuously growing killer cell clone specific for H‐Y antigen

Abstract

H-Y-specific cytotoxic T cells were first cloned in soft agar and grown over a period of 8 months in media conditioned with supernatants from mouse and rat spleen cells stimulated with concanavalin A. The specificity of cloned cells and their cytolytic potential remained essentially unchanged over the entire culture period. In addition to lysing male target cells expressing H-2Db antigens, the cytolytic cells lysed also male as well as female cells expressing H-2Dd alloantigens. Seventeen out of eighteen subclones derived from the original clone revealed the same activity. The cells divide about every 17--20 h can be obtained in large quantities.

H‐2 Restriction Pattern of Secondary Influenza Virus‐immune Cytolytic T Cells

“…ERTL tradictory. Similar results have been published for the H-Y system (Von Boehmer et al, 1979), where long-term cultures of CTL clones lyse male syngeneic as well as male and female allogeneic target cells. T-cell-mediated lysis of allogeneic, antigen-presenting target cells has been described using TNP (Pettinelli, Schmitt-Verhulst and Shearer, 1979) or rabies virus (Wiktor, Doherty and Koprowski, 1977) as antigen.…”

“…Cross-reactivity of H-2'' effector cells on unaltered H-2'' target cells has been described earlier (Finberg et al. 1978;Koszinowski et al, 1979;Von Boehmer et al, 1979). but in these reports lysis was independent of the expression of foreign antigens on the surface of H-2'' target cells.…”

Fas involvement in Ca(2+)-independent T cell-mediated cytotoxicity.

“…Cells (except PC60 and its derived clones) were grown in RPMI 1640 medium (Gibco Bio-Cult, Glasgow, Scotland) enriched with 10% FCS (Biological Industries, Beth Halmeck, Israel) and supplemented with 2 x 10 s M 2-ME, penicillin, and streptomycin. PC60 is a bybridoma (12) between a mouse cytotoxic T cell done with antimale D b specificity (13) and a derivative from the rat T lymphoma W/Fu (C58NT)D. PC60 cells and all PC60-derived cells were grown and cloned in DMEM (Gibco Biocult, Uxbridge, UK) enriched with 5% FCS and the additives above. Cytotoxicity tests were carried out in V-shaped wells of 96-well microtiter plates with SlCr-labeled target cells (104 tumor cells or 10 s thymocytes) and effector cells (PC60-derived dl0S cells or peritoneal exudate cells [14]) at the indicated ratios in a total volume per well of 200/A of ILPMI medium with 10% FCS.…”

T-T cell interactions during in vitro cytotoxic T lymphocyte responses. V. Precursor frequencies and specificity of alloreactive helper T cells.

“…For this type of analysis, however, experimentation at the clonal level rather than at the population level is mandatory. In this regard, the frequency analysis of CTLp and specificity analysis of cloned lines of CTL has provided new information on the expression and development of the repertoire of alloreactive, self-major histocompatibility complex-(MHC) restricted and allo-MHC-restricted antigen-specific sets of CTLp (12)(13)(14)(15). However, information is sparse on the frequency and specificity of precursors of helper T lymphocytes (HTLp) required for differentiation and proliferation of CTLp (16).…”