Fine structural aspects of the development and aging of the tracheal epithelium of mice.

Kawamata, Seiichi; Fujita, Hisao

doi:10.1679/aohc.46.355

Cited by 32 publications

(18 citation statements)

References 19 publications

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“…Of two non-ciliated cell types in mouse tracheal epihelium, KAWAMATA and FUJITA (1983) considered that the SER rich cell was derived from the GER rich cell. Brush cells, observed fairly frequently in the calf, were also reported for the canine vomeronasal organ (ADAMS and WIEKAMP, 1984) and calf bronchi (ALLAN, 1978).…”

Section: Discussionmentioning

confidence: 99%

The bovine vomeronasal organ.

Adams

1986

Arch. Histol. Jap., Arch. Histol. Jpn

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Section: Discussionmentioning

confidence: 99%

The bovine vomeronasal organ.

Adams

1986

Arch. Histol. Jap., Arch. Histol. Jpn

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“…al., 1974;Burri, 1974), dog (Boyden and Tompsett, 19611, pig (Baskerville, 1976), nonhuman primates (Boyden, 1976;Kerr et al, 1975;Bunton and Plopper, 1984;Hislop et al, 1984), and humans (Loosli and Potter, 1959;Campiche et al, 1963;Boyden, 1974). Tracheal development has been described in the mouse (Kawamata and Fujita, 1983), hamster (McDowell et al, 1985a, b;Coleman and McDowell, 1985;Emura and Mohr, 1975), and rhesus monkey (Plopper et al, 1986). Airway branching patterns during development in humans are discussed by Hislop et al (1972), Bucher and Reid (19611, Hislop and Reid (19741, Boyden and Tompsett (19651, and Boyden (1977).…”

Section: Introductionmentioning

confidence: 99%

Morphogenesis of the respiratory bronchiole in rhesus monkey lungs

et al. 1988

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The epithelium of the respiratory bronchiole in the adult rhesus monkey consists of two populations: a pseudostratified epithelium with basal, mucous goblet, and ciliated cells located near the pulmonary artery (PA); and a simple cuboidal epithelium composed only of nonciliated bronchiolar epithelial (or Clara) cells in areas away from the PA. This study describes the pattern of differentiation of these two epithelial populations, and their relationship to the PA and to the time of appearance of alveoli in the respiratory bronchiole of the rhesus monkey during the period of 90-125 days gestational age (DGA). These events were related to changes in the adjacent parenchyma. Dissected airways of infusion-fixed, critical-point-dried lungs were evaluated by scanning microscopy followed by light microscopy of the same airways. At 54% of gestation (90 DGA), the distal airway was lined by a mixture of ciliated and nonciliated cells. By 67% of gestation (110 DGA), the ciliated cells were confined to the epithelium over the PA. The underlying connective tissue initially was cellular containing few fibers but was fibrous by 76% of gestation (125 DGA). Alveolarization began near the most distal cartilage at 57% of gestation (95 DGA), the same period at which secondary septation occurred in the distal acinus. Thus, alveolarization occurred simultaneously in two centers: 1) the proximal centriacinar region in the vicinity of the most distal cartilage and 2) the distal lung parenchyma. The duration of centriacinar alveolarization was short, approximately 5 days.

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“…The differentiation of tracheal epithelium has been previously studied by electron microscopy in the rat (Jeffery and Reid, 1977;Cireli, 1966;Smolich et al, 1967;Kober, 1975), mouse (Kawamata and Fujita, 1983), and rabbit (Leeson, 1961;Kanda and Hilding, 1968). When these studies are compared to the present study and to histological studies in humans (Thurlbeck et al, 1961;de Haller, 1969;Bucher and Reid, 1961a,b;Sturgess, 1985), differences in the time course and sequence of maturation are seen.…”

Section: Discussionmentioning

confidence: 99%

“…4Jeffery andReid (1977); Cireli (1966); Smolich et al (1967);Kober (1975). 'Kawamata and Fujita, 1983. event occurs early in lung development in primates and much nearer parturition in the rat, mouse, and rabbit. However, the secretory cells in none of these nonprimate species are mucous cells in the adult.…”

Section: Discussionmentioning

confidence: 99%

“…Ciliogenesis has been studied in the rat (Sorokin, 1968). The ultrastructural features of overall tracheal epithelial differentiation have been described in the rabbit (Leeson, 1961), mouse (Kawamata and Fujita, 1983), hamster (Emura and Mohr, 1975;Emura et al, 1976, 19771, andrat (Cireli, 1966). Since several recent comparative studies (Plopper et al, 1983~;Jeffery, 1983;Spicer et al, 1983) have suggested that these small laboratory mammals are not adequate models for the study of human tracheobronchial epithelium, the present study was designed to characterize the differentiation of the tracheal epithelial population of a nonhuman primate, the rhesus monkey, from early fetal age to adulthood.…”

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confidence: 97%

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Differentiation of tracheal epithelium during fetal lung maturation in the rhesus monkey Macaca mulatta

1986

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Of the eight categories of epithelial cells identified in pulmonary conducting airways, four are found in the trachea of adult primates: basal, mucous goblet, intermediate, and ciliated cells. While their ultrastructure is well characterized, little is understood about their origin or differentiation. This study describes the pattern of differentiation of the tracheal luminal epithelium in a species of nonhuman primate, the rhesus monkey, Macaca mulatta. Tracheas of 57 fetal and postnatal rhesus were fixed with glutaraldehyde/paraformaldehyde: ten at 29-54 days gestational age (GA), ten at 59-80 days GA (pseudoglandular stage), sixteen at 82-130 days GA (canalicular stage), ten at 141-168 days GA (saccular stage), eight at 1-134 days postnatal, and three adults (2 yr 11 months to 11 yr 11 months). Slices taken proximal to the carina were processed for electron microscopy by a selective embedding procedure. In the youngest fetuses, essentially one population of cells lined the tracheal epithelial surface. These cells were columnar in shape with a central nucleus, few organelles, and large amounts of cytoplasmic glycogen. At 46 days GA, ciliated cells were observed on the membranous side of the trachea. Some nonciliated cells had concentrations of organelles in the most apical portion of their cytoplasm. At 59 days GA, membrane-bound cored granules were intermixed with organelles in the apices of some glycogen-filled cells. They were observed first on the cartilaginous side. Between 59 and 100 days GA, a large number of cell forms which appeared to be transitional between ciliated, secretory, basal, and undifferentiated cells were present. These included ciliated cells with electron-lucent inclusions resembling mucous granules. Mucous secretory cells were more numerous and had more granules and less glycogen in older fetuses. By 105 days GA, few of the secretory cells had significant amounts of glycogen and the cytoplasm was condensed. Secretory granules were very abundant in some cells and minimal in others. The Golgi apparatus was prominent. In animals 120 days GA and older, small mucous granule cells and basal cells resembling these cells in adults were present. By 134 days postnatal age, the epithelium resembled that in adults. We conclude that most of the differentiation of tracheal epithelium in the rhesus monkey occurs prior to birth; the cells differentiate in the following sequences: ciliated, mucous goblet, small mucous granule, basal; and basal and small mucous granule cells do not play a role in ciliated and mucous cell formation in the fetus.

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Fine structural aspects of the development and aging of the tracheal epithelium of mice.

Cited by 32 publications

References 19 publications

The bovine vomeronasal organ.

The bovine vomeronasal organ.

Morphogenesis of the respiratory bronchiole in rhesus monkey lungs

Differentiation of tracheal epithelium during fetal lung maturation in the rhesus monkey Macaca mulatta

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