Fine structural aspects on the cold follicles in the aged mouse thyroid.

Tamura, Shigehiko; Fujita, Hisao

doi:10.1679/aohc1950.44.177

Cited by 22 publications

(7 citation statements)

References 19 publications

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“…At the ultrastructural level, both in SO and in Orx middleaged rats, besides numerous follicular cells with typical organization of cell organelles, quite a few thyrocytes were characterized by dilated and distended RER cisternae. Similar ultrastructural changes were previously reported in thyrocytes of aged mouse thyroid (Tamura & Fujita 1981, Nève & Rondeaux 1991. Tamura & Fujita (1981) described such ultrastructural changes in thyrocytes of 'active' follicles as degenerative.…”

Section: Discussionsupporting

confidence: 86%

“…Similar ultrastructural changes were previously reported in thyrocytes of aged mouse thyroid (Tamura & Fujita 1981, Nève & Rondeaux 1991. Tamura & Fujita (1981) described such ultrastructural changes in thyrocytes of 'active' follicles as degenerative. Both groups related them to follicular cell hyperactivity explained with compensation for lack of TH production in inactive, so-called 'cold' mice follicles.…”

Section: Discussionsupporting

confidence: 86%

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Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity

Šošić‐Jurjević

Filipović

Renko

et al. 2012

Journal of Endocrinology

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Endogenous androgens are involved in regulation of thyroid function and metabolism of thyroid hormones. As serum testosterone level progressively declines with age, this regulation may change. We tested how androgen deprivation, achieved by orchidectomy, affects thyroid homeostasis in middle-aged rats. Fifteen-month-old Wistar rats were orchidectomized (Orx) or sham-operated under ketamine anesthesia (15 mg/kg body weight). Five weeks after the surgery, animals were decapitated. Thyroids were used for histomorphometric and ultrastructural examinations and together with livers and pituitaries for real-time quantitative PCR and deiodinase (DIO) activity measurements. Serum testosterone, TSH, L-thyroxine (T 4 ), and cholesterol (Chol) levels were determined. As expected, middle-aged control rats had lower (P!0 . 05) testosterone and T 4 compared with 3-month-old males. In the Orx middle-aged group, we detected diminished serum testosterone (P!0 . 05), no change in TSH and T 4 levels, and higher Chol level (P!0 . 05), in comparison with age-matched controls. Histomorphometric analysis of thyroid tissue revealed decreased relative volume densities of follicles and colloid (P!0 . 05). Relevant gene expressions and DIO1 enzyme activity were not changed in the thyroids of Orx rats. Liver Dio1 gene expression and DIO1 activity were decreased (P!0 . 05) in comparison with the control values. Pituitary levels of TSHb, Dio1, and Dio2 mRNAs did not change, while DIO2 activity decreased (P!0 . 05). In conclusion, orchidectomy of middle-aged rats affected thyroid structure with no effect on serum T 4 and TSH. However, decreased liver DIO1 and pituitary DIO2 enzyme activities indicate compensatoryadaptive changes in local T 3 production.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 86%

Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity

Šošić‐Jurjević

Filipović

Renko

et al. 2012

Journal of Endocrinology

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“…In some follicles, however, the epithelial cells were relatively flat with a cell height of 3 pm or less . In this study the cells were small, resembling those in Figures 5 and 6 of Many et al (1986a) and differing from those in Figure lb,c of Tamura and Fujita (1981) in which the cells were attenuated (stretched in the direction of the follicle wall). Both of these types of follicles bounded by flat cells resemble the "cold" follicles previously described (Studer et al, 1978;Peter et al, 1982;Denef et al, 1989).…”

Section: Colloid Goiter and Cold Folliclessupporting

confidence: 45%

Histologic changes in tissue components of the hyperplastic thyroid gland during its involution in the rat

1990

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Male Fischer rats were fed a low-iodine diet containing thiouracil for 21 days to produce hyperplastic thyroid glands, and then fed a high-iodine diet for various time intervals, from 5 hr to 180 days, in order to study the morphological changes that occur during involution. Thyroids were fixed by perfusion fixation and embedded in Epon. Sections were examined by light microscopy. Initially at 0 days of involution (at the time of the change to the high-iodine diet), follicular lumens were very narrow and capillary lumens were very wide. The capsule was thick and infiltrated with mononuclear leukocytes. No obvious changes occurred for 1 day after the change in diet, but shortly thereafter capillary lumens began to narrow. By 4 days, most capillary lumens were close to normal size; capillaries formed a more or less normal bed except that many were embedded in a relatively thick or wide interfollicular matrix. This matrix was largely gone by 21 days. Between 1 and 21 days, follicular lumens dilated progressively as colloid accumulated. The density of staining of the accumulated colloid varied from follicle to follicle, and this variation was also observed in older controls. Inflammatory cells gradually disappeared from the capsule and most were gone by 15 days. Starting at approximately 15 days and continuing to 180 days, one or more disintegrating cells were found in some lumen profiles. Colloid goiters were not observed in these rats even after several months of involution. Some lumens were rather large, however, and small fractions of the follicles, both small and large, were bounded by flat cells and resembled "cold" follicles morphologically.

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“…Besides the evidence that a significant accumulation of lysosomes in follicular epithelial cells is considered as a morphological marker of thyroid aging in different species (Studer et al, 1978;Tamura and Fujita, 1981), this phenomenon was also noticed in younger animals under special experimental conditions (Krupp et al, 1983).…”

Section: Introductionmentioning

confidence: 82%

Effect of estradiol and progesterone on thyroid gland in pigs: A histochemical, stereological, and ultrastructural study

Sekulić¹,

Šošić‐Jurjević²,

Filipović³

et al. 2006

Microscopy Res & Technique

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The cellular and subcellular organization of thyroid follicular cells in peripubertal (6-month-old) male pigs treated with estradiol-dipropionate (Oe) plus progesterone (Pr) in combination on the first postnatal day was studied. A stereological method was used for morphometric determinations of the relative volume densities of the follicular epithelium, colloid and interstitium, and for establishing the epithelial height and index of activation rate. Statistically significant differences of the examined parameters between the control and Oe+Pr -treated groups were determined by Student's t-test. The subcellular organization of thyroid follicular cells was studied using transmission electron microscopy (TEM). When compared with the control group, in the Oe+Pr -treated pigs, thyroid follicles possessed a noticeably higher follicular epithelium when compared with the control animals. The observed changes were quantified and comparison between the experimental groups showed that the height, volume density of follicular epithelium, and index of activation rate were significantly (P < 0.05) increased, whereas the relative volume density of the colloid was significantly (P < 0.05) decreased. At the subcellular level the thyroid follicular cells of Oe+Pr -treated pigs were characterized by increased number of polysomes and dense bodies and extensive endoplasmatic reticulum. It was concluded that a single neonatal treatment with female gonadal steroids exerted a prolonged effect on the pig's thyroid, characterized by increased biosynthesis and reabsorption of the colloid by the follicular cells.

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Fine structural aspects on the cold follicles in the aged mouse thyroid.

Cited by 22 publications

References 19 publications

Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity

Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity

Histologic changes in tissue components of the hyperplastic thyroid gland during its involution in the rat

Effect of estradiol and progesterone on thyroid gland in pigs: A histochemical, stereological, and ultrastructural study

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