Fine structural differences in the amoebocytes of Biomphalaria glabrata

Joky, Alexandre; Matricon-Gondran, Michelle; Benex, J

doi:10.1016/0145-305x(83)90090-3

Cited by 20 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Heterogenity of these cells was confirmed morphologically (Sminia 1981, Joky et al 1983, Barraco et al 1993, Bezerra et al 1997, Matricon-Gondran & Letorcart 1999, Johnston & Yoshino 2001, and functionally (Granath & Yoshino 1983, Matricon-Gondran & Letorcart 1999b, Martins-Souza et al 2003. Our results confirmed that circulating hemocytes express ligands (carbohydrates) for lectin binding in a very heterogeneous manner.…”

Section: Discussionmentioning

confidence: 94%

Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection

Martins-Souza¹,

Pereira²,

Filho

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 94%

Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection

Martins-Souza¹,

Pereira²,

Filho

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

“…Among these, one cell type was found to share typical properties of mammalian macrophages, such as high phagocytosis activity, resistance to elevated doses of gamma radiation and the presence of inner lysosomal structures (Bezerra et al 2003). An even higher number of hemocyte types were described by Joky et al (1983). Up to four cell types were proposed based not only on morphological characteristics, but also on their ability of expressing different surface lectin receptors.…”

Section: Discussionmentioning

confidence: 99%

Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

Barbosa

Silva

Coelho³

et al. 2006

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Biomphalaria glabrata snails are major hosts for the digenetic trematoda Schistosoma mansoni, the causative agent of human schistosomiasis. The success or failure of the infection will be dependent on the mobilization of the molluskan internal defense system, where a major role will be played by circulating hemocytes produced by the APO (amebocyte-producing organ) Schistosomiasis is a parasitic infection caused by the digenetic trematode Schistosoma mansoni Sambom, 1907. The disease is prevalent in 54 countries and territories of Africa, the Caribbean, the Mediterranean, and South America. It is estimated that 130 million people are infected with this species of parasite (Chitsulo et al. 2000). Although several advances have been achieved in the control of the schistosomiasis morbidity, mainly due to the development of new low toxicity and highly effective drugs against the parasite, it is still an endemic disease, whose control and/or eradication is very difficult in the wide regions where it occurs. The affected patients become debilitated, thus impairing the socio-economic development of the affected countries. In Brazil, it is estimated that eight million people are infected, whereas thirty million are at high risk of infection, since they live in hyper endemic areas (Katz 1997 In the S. mansoni-Biomphalaria interaction, besides the stringent physiological and biochemical compatibility between host and parasite, the success or failure of the infection will be strongly determined by the efficacy of the internal defense system of the snail. When in contact with the infective agent, the endogenous defense mechanisms of the mollusk are immediately activated, mediated by circulating effectors cells (hemocytes) and by soluble factors which are present in the hemolymph. Among these factors, lectins with specific carbohydratebinding capacities play an important role by immobilizing foreign objects by agglutination, promoting phagocytes by the hemocytes or even acting as cytophilic receptors for non-self recognition (Van der Knaap & Loker 1990).The hemocytes of Biomphalaria are capable of phagocytosis, encapsulation and, finally, destruction of the infective agents. Both morphologically and functionally, these cells resemble cells of the vertebrate monocytemacrophage series (Jeong et al. 1983, Bezerra et al. 2003. Experimental evidence has been accumulated assigning the production and storage of hemocytes to the hematopoietic organ, a structure that is located on the wall of the pericardium and posterior epithelium of the mollusk mantle. That organ, also known as amebocyte-producing organ or APO, is composed of a small quantity of primary ameboblasts that rest on a loose reticulum, which is formed by extensions of smooth muscles and fibroblasts (Jeong et al. 1983). In a recent study, Sullivan et al. (2004) reported an increase in the frequency of cell mitosis in the APO of B. glabrata following the inoculation of miracidia or cercariae antigens of S. mansoni. The mitotic ratio was intensified in Biomphalaria strai...

show abstract

“…) выделяют два основных морфологических типа гемоцитов: гиалиноциты, определяемые как малогранулярные (или негранулярные) клетки и гранулоциты (клетки, цитоплазма которых содержит многочисленные гранулы и везикулы). Такое различие клеток гемолимфы отмечено как на световом, так и на электронномикроскопическом уровнях (Joky et al, 1983;Ottaviani, Franchini, 1988;Cueto et al, 2015).…”

Section: Introductionunclassified

“…Однако морфологические критерии классификации клеток гемолимфы пока сформулированы не четко, что во многом обусловлено полиморфизмом гемоцитов. Поэтому в ряде работ (Joky et al, 1983;Cavalcanti et al, 2012;Cueto et al, 2015) наряду с этими основными морфотипами выделяются другие формы клеток (в основном гранулярных). Другой причиной, затрудняющей классификацию гемоцитов, является слабая изученность их онтогенетических и функциональных изменений.…”

Section: Introductionunclassified

The analysis of cell composition in the hemolymph of three Planorbidae species (Gastropoda: Pulmonata)

et al. 2018

View full text Add to dashboard Cite

РЕЗЮМЕ: Изучен клеточный состав гемолимфы трёх видов пульмонат семейства Planorbidae: Biomphalaria glabrata, Planorbarius corneus и Planorbis planorbis. По данным морфологического, морфометрического и цитофлуориметрического анализов гемоциты этих моллюсков представлены популяцией гиалиноцитов и гетерогенной фракцией, состоящей из двух популяций гранулоцитов-крупных и мелких клеток. С использованием специфического лизосомного красителя LysoTracker и красителя нуклеиновых кислот SYTO62 в составе каждой популяции определены четыре субпопуляции клеток. Между клетками, относящимися к разным морфологическим популяциям, обнаружены достоверные различия в содержании лизосом и накоплении нуклеинового красителя. Гиалиноциты содержат меньшее количество лизосом, чем гранулоциты. При этом крупные гранулоциты характеризуются наибольшей концентрацией лизосом и метаболической активностью, а мелкие сильнее варьируют по степени гранулярности и более гетерогенны по составу гранул.

show abstract

Fine structural differences in the amoebocytes of Biomphalaria glabrata

Cited by 20 publications

References 7 publications

Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection

Differential lectin labelling of circulating hemocytes from Biomphalaria glabrata and Biomphalaria tenagophila resistant or susceptible to Schistosoma mansoni infection

Primary culture of the region of the amebocyte-producing organ of the snail Biomphalaria glabrata, the intermediate host of Schistosoma mansoni

The analysis of cell composition in the hemolymph of three Planorbidae species (Gastropoda: Pulmonata)

Contact Info

Product

Resources

About