Fine-Structure Molecular Typing of Irish
            <i>Helicobacter pylori</i>
            Isolates and Their Genetic Relatedness to Strains from Four Different Continents

Carroll, Ian M.; Ahmed, Niyaz; Beesley, Sarah M.; Khan, Aleem Ahmed; Ghousunnissa, Sheikh; Moráin, Colm A. O; Smyth, Cyril J.

doi:10.1128/jcm.41.12.5755-5759.2003

Cited by 15 publications

(13 citation statements)

References 23 publications

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“…Another interesting observation based on ERIC typing was the distinctiveness of Irish isolates. This is yet more evidence to confirm previous findings, which argued that Irish isolates have certain distinctive features compared to isolates from other European countries (7). These features include weakly clonal population structure, homogeneous fingerprinting profiles from unrelated individual isolates, and evolutionary links with both East Asian and Northern European isolates.…”

Section: Discussionsupporting

confidence: 79%

“…Phylogenetic analysis revealed interesting links between isolates from Japan and Peru, contrasting with earlier observations established with the same set of strains (17). Our results based on ERIC typing were also not in agreement with recent observations of Japanese and Peruvian isolates based on FAFLP analysis, in which Peruvian isolates were found to be phylogenetically more closely related to Spanish isolates than to Japanese isolates (7). However, the number of isolates analyzed in that study was insufficient to establish conclusive data (only one Japanese isolate was studied) (7).…”

Section: Discussioncontrasting

confidence: 57%

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Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution

et al. 2004

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Biogeographic partitioning of the genome is typical of the gastric pathogen Helicobacter pylori. Such population-specific evolution could serve as a model for understanding host-pathogen interaction and the impact of genetic drift and recombination on insular populations. With a total of 320 isolates from six geographic regions (Japan, India, England, Spain, Ireland, Africa, and Peru) analyzed by enterobacterial repetitive intergenic consensus (ERIC)-based genotyping, we examined genetic affinities among various H. pylori populations in the world. Several strain-specific and region-specific differences were observed by ERIC-based typing. Polymorphic ERIC patterns indicated that the ERIC sequences are in fact dispersed in the H. pylori chromosome at different locations separated by various distances. Phylogenetic analysis of 61 representative isolates revealed three distinct genetic clusters populated by isolates with shared ERIC types independent of the cag right-junction motif type and vacA allele status. Among the notable genetic relationships were the genotypic similarities between Irish and Japanese and between Peruvian and Japanese isolates. Insular genotypic characteristics of Irish isolates amid genetic similarity to East Asian, as well as North European, strains have been once again proved in this study. Peruvian genotypes were more similar to those of Japanese isolates than to those of Iberian or European isolates. Given the current debate on the origin and age of present-day H. pylori, this is a significant finding that supports the possibility of ancient colonization of Amerindians with East Asian strains. Genotypic data presented here will be additionally helpful in realizing the importance of H. pylori geographical genomics in the development of gastroduodenal pathology. Stomach infection withHelicobacter pylori is the second most common infectious disease of humans. The severe pathological consequences of this infection include gastric and duodenal ulcer disease, the development of gastric mucosal atrophy, gastric carcinoma, and, more rarely, malignant tumors of the lymphoma (12,14,15,17,18). H. pylori infections cause very high morbidity and mortality and are of particular concern in developing countries, where H. pylori prevalences as high as 90% have been reported (6,20,31).H. pylori isolates obtained from different individuals and ethnic groups in the world exhibit substantial genomic diversity due to synonymous substitutions, insertion-deletion (indel) polymorphisms, and mobility of repetitive elements. This diversity could be further enhanced by chromosomal rearrangements due to a high level of interstrain recombination. Geographical partitioning of the gene pool exists within H. pylori, and sequences are less related between isolates from different populations than between isolates from families (1). Several molecular typing tools were tried for strain typing and identification of H. pylori isolates. These include pulsed-field gel electrophoresis (32), random fragment length polymorphism (28...

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Section: Discussionsupporting

confidence: 79%

Section: Discussioncontrasting

confidence: 57%

Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution

et al. 2004

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“…Whole-genome fingerprinting based on fluorescent amplified fragment length polymorphism (FAFLP) genotyping was done as described previously (2,5). Briefly, the profiling of whole-genome microrestriction fingerprints with EcoRI/ MseI enzymes using the fluorescence-tagged primer pairs EcoRIϩA-MseIϩ0 and EcoRIϩG or A-MseIϩ0 was performed for all 10 strains.…”

Section: Methodsmentioning

confidence: 99%

Comparing Genomes of Helicobacter pylori Strains from the High-Altitude Desert of Ladakh, India

et al. 2005

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The genomic diversity of Helicobacter pylori from the vast Indian subcontinent is largely unknown. We compared the genomes of 10 H. pylori strains from Ladakh, North India. Molecular analysis was carried out to identify rearrangements within and outside the cag pathogenicity island (cag PAI) and DNA sequence divergence in candidate genes. Analyses of virulence genes (such as the cag PAI as a whole, cagA, vacA, iceA, oipA, babB, and the plasticity cluster) revealed that H. pylori strains from Ladakh are genetically distinct and possibly less virulent than the isolates from East Asian countries, such as China and Japan. Phylogenetic analyses based on the cagA-glr motifs, enterobacterial repetitive intergenic consensus patterns, repetitive extragenic palindromic signatures, the glmM gene mutations, and several genomic markers representing fluorescent amplified fragment length polymorphisms revealed that Ladakhi strains share features of the Indo-European, as well as the East Asian, gene pools. However, the contribution of genetic features from the Indo-European gene pool was more prominent.

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“…Many factors and cofactors influence gastric cancer development [8,10,[32][33][34]. Helicobacter pylori virulence is under the control of Helicobacter pylori gene factors [25,27,30,31]. There is actually an important change in the relationships between helicobacter pylori and humans, caused by modern life change in gut micro-ecology [19,29].…”

Section: Helicobacter Pylori Infectionmentioning

confidence: 99%

“…It's widely involved in gastritis, asymptomatic for most infected individuals. A small proportion of infected individuals develop more dangerous gastritis involving peptic ulceration and gastric malignancy [22,[25][26][27][28][29][30][31][32][33]. Helicobacter pylori is responsible for tremendous morbidity and mortality throughout the world [6,7,12,[14][15][16].…”

Section: Helicobacter Pylori Infectionmentioning

confidence: 99%

Meanders of an Atypical Research Work on Helicobacter pylori in the Democratic Republic of Congo: Influence of HIV and other Factors and Study of some Interesting Cases

NdelodiPhanzu¹,

Malolo²,

Matondo³

et al. 2018

J Pharmacogenomics Pharmacoproteomics

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During the 3 rd Euro case reports in Valencia, Spain, a strange medical case taking place in the DRC has been presented. Indeed, while the Congolese population is, until now, convinced of the existence of massive criminal poisonings, throughout the national area, originating from the East region of the country, we incredibly observed and demonstrated that actually, the phenomenon is a silent, surprising and unexpected Helicobacter pylori epidemic with numerous extra-digestive pathologies. The present subject will deal with picturesque meanders by which our research work passed, lengthening it to more than twenty years before getting appropriate results. The presentation will start by a brief summary of the DRC Helicobacter pylori phenomenon described in Valencia and hypothesis raised for better knowledge of Helicobacter pylori infection, at the international level. In a second time, it will focus on the influence and consequences of some followed factors in our study: HIV infection, political situation in DRC, lack of equipment and financial means, population ignorance, traditional practitioners' cupidity, deficiency of south-North collaboration, and so on. Afterwards, two interesting incredible extra-digestive DRC Helicobacter pylori cases observed in our study will be discussed. Finally a brief conclusion will present some useful propositions drawn from the experience of this DRC Helicobacter pylori research work.

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Fine-Structure Molecular Typing of Irish Helicobacter pylori Isolates and Their Genetic Relatedness to Strains from Four Different Continents

Abstract: Genotyping of 74 Irish Helicobacter pylori isolates was performed at four different loci (vacA signal sequence and mid-region, insertion-deletion polymorphisms at the 3 end of the cag pathogenicity island, and cagA).

Cited by 15 publications

References 23 publications

Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution

Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution

Comparing Genomes of Helicobacter pylori Strains from the High-Altitude Desert of Ladakh, India

Meanders of an Atypical Research Work on Helicobacter pylori in the Democratic Republic of Congo: Influence of HIV and other Factors and Study of some Interesting Cases

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