Summary Chromosome analysis was performed in six grasshopper species of the family Acrididae, viz., Acrida exaltata (2n=23+XO), Phlaeoba antennata (2n=23+XO), Gastrimargus africanus africanus (2n=23+XO), Parahieroglyphus bilineatus (2n=23+XO), Cyrtacanthacris tatarica (2n=23+XO), and Hieroglyphus banian (2n=23+XO), with X chromosome being the largest in the complement. Detailed karyotypic information regarding chromosome length, total complement length of chromosome set, and relative length were measured using the Micromeasure software.