Fine structure of human immunodeficiency virus (HIV), immunolocalization of structural proteins and virus-cell relation

Monoclonal Antibodies Directed against Human Immunodeficiency Virus (HIV) gag Proteins with Specificity for Conserved Epitopes in HIV-1, HIV-2 and Simian Immunodeficiency Virus

“…In immunoelectron microscopy (IEM) the incubation of ultrathin cryostat sections with anti-p 17 and anti-p24 MAbs led to a labelling of internal viral components (Fig. 2 a, b, c) thus corroborating earlier observations on the localization of the structural proteins (Gelderblom et al, 1987(Gelderblom et al, , 1988. In pre-embedding IEM, a technique allowing the exclusive detection of external antigens, no labelling was observed with these antibodies (data not shown).…”

Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

“…Eccentric electron-dense bodies were also observed in some particles. These morphological characteristics are very similar to those of mature H I V particles (Munn et al, 1985;Palmer et al, 1985;Meyenhofer et al, 1987;Gelderblom et al, 1988Gelderblom et al, , 1989Hockley et al, 1988). Mature HIV-like particles connected to the cell surface were not identified by transmission electron microscopy.…”

“…Mature virions with an electron-dense core have been observed in the culture medium of HIV-infected cells (Gonda et al, 1985;Munn et al, 1985;Palmer et al, 1985;Meyenhofer et al, 1987;Gelderblom et al, 1988Gelderblom et al, , 1989. Morphologically similar particles were identified in the extracellular space of cells infected with the m0GV and m0G5 constructs which encode the protease gene ( Fig.…”

“…Morphologically, HIV-1 and -2 resemble visna virus (Gonda et al, 1985 ;Munn et al, 1985); mature virions of .HIV have an electron-dense and bar-shaped core (Klatzmann et al, 1984;Munn et al, 1985;Palmer et al, 1985;Meyenhofer et al, 1987;Gelderblom et al, 1988Gelderblom et al, , 1989Hockley et al, 1988). Immature particles with a doughnut-shaped core have also been observed extracellularly in HIV-infected cell cultures (Munn et al, 1985;Palmer et al, 1985;Gelderblom et al, 1988Gelderblom et al, , 1989Hockley et al, 1988).…”

Human retroviruses and AIDS 1997

“…From cross-linking and molecular-genetic experiments both trimer-and dimerformation have been reported . From morphologic studies a trimeric shape of the knobs became evident (Gelderblom et al, 1987a;Gelderblom et al, 1988;Grief etal., 1989).…”