1994
DOI: 10.1002/jemt.1070290106
|View full text |Cite
|
Sign up to set email alerts
|

Fine structure of Tritrichomonas foetus as seen using cryotechniques

Abstract: Tritrichomonas foetus was studied using different physical and chemical fixation methods such as fast-freezing (by high pressure, "slam-freezing," and jet-propane), freeze-substitution, conventional freeze-fracture and deep-etching, cryoultramicrotomy, and routine preparation for transmission electron microscopy. The use of fast-freezing fixation (FFF) proved to be superior in terms of structural preservation due to the rapidity of this fixation compared to that obtained using conventional chemical fixation. T… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
2
0

Year Published

1998
1998
2005
2005

Publication Types

Select...
4
1

Relationship

1
4

Authors

Journals

citations
Cited by 6 publications
(2 citation statements)
references
References 19 publications
0
2
0
Order By: Relevance
“…A maximum of 10–15 μm of well‐frozen sample in contact with the cryogen is the most that can be achieved. In contrast, high‐pressure freezing has the potential to freeze up to 200 μm of biological samples without apparent ice‐crystal artefacts ( Studer et al ., 1989 ; Sartori et al ., 1993 ) and offers the morphologist the ability to retain excellent cellular ultrastructure ( McDonald & Morphew, 1993; Benchimol, 1994) and the immunocytochemist a way to avoid aldehyde fixatives ( Young et al ., 1995 ). However, surprisingly few reports show immunocytochemical labelling of high‐pressure frozen material.…”
Section: Introductionmentioning
confidence: 99%
“…A maximum of 10–15 μm of well‐frozen sample in contact with the cryogen is the most that can be achieved. In contrast, high‐pressure freezing has the potential to freeze up to 200 μm of biological samples without apparent ice‐crystal artefacts ( Studer et al ., 1989 ; Sartori et al ., 1993 ) and offers the morphologist the ability to retain excellent cellular ultrastructure ( McDonald & Morphew, 1993; Benchimol, 1994) and the immunocytochemist a way to avoid aldehyde fixatives ( Young et al ., 1995 ). However, surprisingly few reports show immunocytochemical labelling of high‐pressure frozen material.…”
Section: Introductionmentioning
confidence: 99%
“…Several studies have compared the morphology of cryopreservation methods with conventional glutaraldehyde fixation in nonhematologic tissues (Benchimol, 1994;Meissner and Schwarz, 1990), normal human peripheral blood basophils (Hastie, 1990), and nonhuman lymphocytes (Pfaller and Rovan, 1978). Improved ultrastructural preservation was reported; however, these cells were not analyzed morphometrically nor have human neutrophils been investigated with these two fixation methods.…”
mentioning
confidence: 95%