Fine Structure of the Dome in Peyer's Patches of Mice

Abe, Kazuhiro; Ito, Takashi

doi:10.1679/aohc1950.41.195

Cited by 17 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The morphology of follicles in Peyer's patches is quite comparable to that of the appendix (KIMURA,1977;ABE andITO,1978: LAUSE andBocKMAN,1981). This correlates quite well with evidence for a similar handling for luminal antigens.…”

Section: Functional Considerationsmentioning

confidence: 89%

Functional histology of appendix.

Bockman

1983

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

Section: Functional Considerationsmentioning

confidence: 89%

Functional histology of appendix.

Bockman

1983

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

“…Macrophages are a major component of the intestinal lamina propria (8, 16) but have only rarely been recognized in the epithelium (6,7). Macrophages within the germinal centers contain tangible bodies consisting of condensed nuclei of lymphocytes and other debris in phagosomes (1,17). In both normal and nude mice, the characteristic cytoskeletal remains of the G. muris allowed us to identify the source of phagosome material, which usually is not identiflable.…”

Section: Discussionmentioning

confidence: 99%

Phagocytosis of Giardia muris by macrophages in Peyer's patch epithelium in mice

Owen¹,

Allen²,

Stevens³

1981

Infect Immun

109

View full text Add to dashboard Cite

No mechanism for the initiation of immunological clearance of Giardia from the mammalian intestinal tract has been identified. In normal and nude mice experimentally infected with G. muris, we examined antigen-sampling epithelium over Peyer's patch follicles by electron microscopy for evidence of interaction between G. muris and lymphoid cells. Invading G. muris were found in the epithelium near dying or desquamating columnar cells. Macrophages beneath the basal lamina extended pseudopods into the epithelium, trapping invading G. muris and enclosing them in phagolysosomes. In normal mice, which clear G. muris in 4 to 6 weeks, macrophages containing digested G. muris were surrounded by rosettes of lymphoblasts in the epithelium. In nude mice deficient in lymphocytes, there was apparent hyperplasia of macrophages, which filled the follicle domes, resulting in more frequent entrapment of G. muris but no contact between macrophages and lymphoblasts in the epithelium. In nude mice, which require 6 months to control G. muris infection, lymphoblast contact with macrophages containing distinctive microtubular remnants of G. muris was only identified in the follicle dome. This close physical association of lymphoblasts and macrophages containing G. muris remnants suggests that this macrophage activity represents intraepithelial antigen processing as well as a defense against the effects of the uncontrolled entrance of microorganisms and other antigenic particles into Peyer's patch lymphoid follicles.

show abstract

“…Below the epithelium, B cells and T helper cells in large numbers formed a cellular meshwork through which transported antigen would have to pass. This meshwork also contained macrophages (previously characterized by enzyme cytochemistry and electron microscopy [Abe and Ito, 1978a;LeFevre et al, 197911, and Ia+ cells with dendritic morphology.…”

Section: Discussionmentioning

confidence: 72%

“…B220 is a cell surface antigen found on pre-B cells, small and large B lymphocytes, and some plasma cells (Coffman, 1982); therefore, some B220 + cells may be plasma cells or large lymphocytes which have been found in the dome by histologic and electron microscopic techniques (Abe and Ito, 1978). Presumably, most B220 + cells are small lymphocytes bearing surface Ig since the labeling pattern of B220+ cells in the dome is similar to that of small lymphocytes in the follicle, and since small lymphocytes are common in the dome (Abe and Ito, 1978a). Whether the small lymphocytes possess surface IgG, IgM, or IgA was not determined.…”

Section: Discussionmentioning

confidence: 99%

Differential distribution of lymphocytes and accessory cells in mouse Peyer's patches

Ermak

Owen

1986

Anat. Rec.

View full text Add to dashboard Cite

The aim of this immunohistologic study was to determine how lymphocytes and accessory cells associated with antigen processing are distributed in the domes of Peyer's patches. Monoclonal antibodies against mouse B cells (anti-B220), T cells (anti-Thy-1.2), T helper cells (anti-L3T4), T cytotoxic/suppressor cells (anti-Lyt-2), macrophages (anti-Mac-1), and Ia+ cells (anti-I-Ad) were applied to cryostat sections of mouse Peyer's patches and visualized with peroxidase-conjugated avidin-biotin complexes (ABC). The subepithelial dome, the dome epithelium, and the follicle crypt epithelium were ech surveyed for cells labeled with these antibodies. Each region had a characteristic and nonrandom distribution of labeled cells. In the subepithelial dome, B cells, T helper cells, macrophages, and Ia+ accessory cells formed a cellular meshwork between follicle and the dome epithelium. T cytotoxic/suppressor cells were sparse beneath the epithelium. In the dome epithelium, solitary and paired lymphocytes occurred both above and below the level of epithelial cell nuclei. B cells predominated, and both T helper cells and T cytotoxic/suppressor cells were present. B cells sometimes aggregated in small clusters. Macrophages were rarely observed in the epithelium. The distribution of cells in the dome epithelium was distinctly different from non-Peyer's patch intestine where T cytotoxic/suppressor cells predominated and B cells were few in number. Although lymphocytes were usually absent in crypts outside Peyer's patches, in follicle crypts B cells and T cells were seen but not Ia+ accessory cells or macrophages. Most of the T cells in the crypt epithelium were T cytotoxic/suppressor cells.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Fine Structure of the Dome in Peyer's Patches of Mice

Cited by 17 publications

References 16 publications

Functional histology of appendix.

Functional histology of appendix.

Phagocytosis of Giardia muris by macrophages in Peyer's patch epithelium in mice

Differential distribution of lymphocytes and accessory cells in mouse Peyer's patches

Contact Info

Product

Resources

About