Fine structure of the lysosomes in the two types of synoviocytes of normal rat synovial membrane

Graabæk, Pernille Møller

doi:10.1007/bf00218006

Cited by 16 publications

(15 citation statements)

References 14 publications

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“…The lining layer of the synovial membrane contains two kinds of lining cells: macrophage-like type A and fibroblastic type B cells (Barland et al, 1962;Graabaek, 1984). We were unable to observe all organellae in the ER␣-positive cells due to the use of GMA resin, which is disadvantageous for detailed observations.…”

Section: Discussionmentioning

confidence: 84%

“…Many ultrastructural investigations have pointed out that the synovial membrane consists of two kinds of synovial lining cells: macrophage-like type A and fibroblastic type B cells (Barland et al, 1962;Graabaek, 1984). In rodents, type A cells are characterized by numerous vesicles, vacuoles, and lysosomes, while type B cells possess a well-developed rough endoplasmic reticulum and a number of dense secretory granules.…”

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confidence: 99%

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Expression of estrogen receptor α (ERα) in the rat temporomandibular joint

Yamada¹,

Nozawa-Inoue²,

Kawano³

et al. 2003

Anat. Rec.

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Numerous epidemiological studies have pointed out a higher frequency of temporomandibular disorder (TMD) in women than in men, which indicates the involvement of a sex hormone, such as estrogen, in the pathogenesis of TMD. Although estrogen is known to play pivotal roles in osteoarthrosis or rheumatoid arthritis in systemic joints, there have been few reports about the role of estrogen in the temporomandibular joint (TMJ). The effect of estrogen is generally mediated by the estrogen receptors (ERs) ER␣ (the predominant type) and ER␤. In this study we examined the expression of ER␣ protein and mRNA in the TMJ of adult male rats by immunocytochemistry and in situ hybridization histochemistry. Intense ER␣ immunoreactivity was localized in the synovial lining cells, stromal cells in the articular disc, and chondrocytes in the TMJ. These ER␣-immunopositive synovial lining cells are characteristic of cytoplasmic processes identified with confocal and immunoelectron microscopy, which indicates that they are synovial type B cells. In situ hybridization histochemistry confirmed intense signals for ER␣ in the synovial lining cells and the sublining fibroblasts at mRNA levels. The nuclei of chondrocytes showed an intense immunoreaction for ER␣ in the maturative and hypertrophic layers of the articular cartilage. In addition to the nuclear localization of ER␣, a weak immunoreaction appeared in the cytoplasm of some ER␣-positive cells. These findings support the hypothesis that TMJ tissue-at least in the male rat-has the potential to be an estrogen target tissue. Anat Rec Part A 274A: 934 -941, 2003.

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

Expression of estrogen receptor α (ERα) in the rat temporomandibular joint

Yamada¹,

Nozawa-Inoue²,

Kawano³

et al. 2003

Anat. Rec.

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“…All these observations have led to the classification of synovial cells into two categories seen nearly in all mammalian species so far studied: i) cells resembling histiocytes (A cells, M, macrophage-like, cells) whose phagocytic capacity has been demonstrated earlier (Ball et al 1964;Cochrane et al 1965) and ii) cells with well-developed granular endoplasmic reticulum probably secrening proteins (B cells or F, fibroblast-like, cells or S, secretory, cells) and were first described by Barland et al (1962) and then by Wyllie et al (1964), Krey and Cohen (1973), Johansson and Rejno (1976), Okada et al (1981), Graabaek (1984;1985) and others.…”

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confidence: 88%

The Ultrastructure of Synovial Membrane in the Prenatal Pig

Horký¹

1990

Acta Vet. Brno

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Horky, D.: The Ultrastructure of Synovial Membrane in the Prenatal Pig. Acta vet. Brno, 59, 1990: 13-2l.The synovial membrane from 6 prenatal pigs of both sexes at 57 days after fertilization was studied. Tissue samples for examination by light and electron microscopy were collected in all instances from the capsule of the hip joint.The synovial membrane under study consisted largely of intercellular matter which included cells at early stages of differentiation. These, however, could be distinguished on the basis of their ultrastructure into A and B types. Apart from these clear-cut types, some intermediate types, particularly among B cells, were observed; they were designated types I and II. They differed in the character of their granular endoplasmic reticulum and by the absence of secretory granules while the other characteristics of B cells were preserved. Intracytoplasmic filaments were not demonstrated in any type of the cells; desmosomes connecting the cells were not developed and the basal membrane was missing.The fibrillar component of intercellular matter was represented mainly by aperiodic filaments. Collagen fibrils were few in number and infrequently formed small bundles running in various directions. Occasional collagen fibrils protruded into the articular cavity in the areas of the synovial membrane not covered by fiat protrusions of synovialocytes. Collagen fibrils passing through the cell membrane of B cells were detected. In their vicinity in the ground amorphous matter, bundles of aperiodic filaments were observed. Porcine synovial membrane, synovial cells, synovial matrixSynovial membrane plays a very important role in functioning of the joint in both physiological and pathological conditions and thus investigations into its SUbmicroscopic structure and the ultrastructure of synovialocytes in particular have been carried out in various mammalian and avian species (Barland etal

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“…The type A cell is characterized by numerous vacuoles, lysosomes, nuclei with rich heterochromatin, and cell surface filopodia, while the type B cell possesses a well-developed rough endoplasmic reticulum (rER), a nucleus with less chromatin, and cytoplasmic long projections. In addition, some ultrastructural investigations have pointed out that the fibroblast-like type B cells had invaginations reminiscent of caveolae in their cell membrane (Graabaek, 1984;Iwanaga et al, 2000;Nozawa-Inoue et al, 2003). However, both the nature and the functional significance of this structure remain unclear.…”

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confidence: 99%

Expression of caveolin‐1 in the rat temporomandibular joint

et al. 2005

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This immunocytochemical study revealed the expression of caveolin-1, a major protein of caveolae, in the rat temporomandibular joint. In the synovial lining layer, immunoreactive products for caveolin-1 were detected on the cell membrane of the fibroblast-like type B cells, as confirmed by immunocytochemistry for heat shock protein 25. The cells in the articular disk, the articular layer, and zone of proliferation of the mandibular condyle also showed intense immunoreactions for caveolin-1.

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Fine structure of the lysosomes in the two types of synoviocytes of normal rat synovial membrane

Cited by 16 publications

References 14 publications

Expression of estrogen receptor α (ERα) in the rat temporomandibular joint

Expression of estrogen receptor α (ERα) in the rat temporomandibular joint

The Ultrastructure of Synovial Membrane in the Prenatal Pig

Expression of caveolin‐1 in the rat temporomandibular joint

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