Fine Structure of Transplantable Hepatomas of the Rat2

Hruban, Z.; Swift, Hewson; Rechcigl, Miloslav

doi:10.1093/jnci/35.3.459

Cited by 91 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These criteria conform to published morphologic data for the identification of microbodies in rat liver (2)(3)(4)(5)(6)(7)(8)(9)15) . Furthermore, in all reacted material not a single normal-appearing, unstained microbody was observed, thus indirectly implying that the stained organelles must be the microbodies.…”

Section: Identification Of Microbodiessupporting

confidence: 70%

Cytochemical Localization of Peroxidatic Activity of Catalase in Rat Hepatic Microbodies (Peroxisomes)

Fahimi¹

1969

The Journal of Cell Biology

275

104

View full text Add to dashboard Cite

Prominent staining of rat hepatic microbodies was obtained by incubating sections of aldehyde-fixed rat liver in a modified Graham and Karnovsky's medium for ultrastructural demonstration of peroxidase activity . The electron-opaque reaction product was deposited uniformly over the matrix of the microbodies . The microbodies were identified by their size, shape, presence of tubular nucleoids, and other morphologic characteristics, and by their relative numerical counts . The staining reaction was inhibited by the catalase inhibitor, aminotriazole, and by KCN, azide, high concentrations of H202, and by boiling of sections . These inhibition studies suggest that the peroxidatic activity of microbody catalase is responsible for the staining reaction . In the absence of exogenous H202 appreciable staining of microbodies was noted only after prolonged incubation .

show abstract

Section: Identification Of Microbodiessupporting

confidence: 70%

Cytochemical Localization of Peroxidatic Activity of Catalase in Rat Hepatic Microbodies (Peroxisomes)

Fahimi¹

1969

The Journal of Cell Biology

275

104

View full text Add to dashboard Cite

show abstract

“…The ultrastructure of a series of rat hepatomas is known from this study and from previous reports [5,6,8,24,32,46]. It has been de monstrated previously, that hepatic catalase is localized in the matrix and uricase in the crystalloid of microbodies [23].…”

Section: Regulation Of Catalase Levels In the Neoplastic Tissuessupporting

confidence: 58%

“…The demonstration in this laboratory [43] of two lines of trans plantable hepatomas with widely différent catalase activity, one exceeding the level seen in the liver, coupled with the reports of relatively high catalase activity in a number of other transplanted as well as primary tumors [24,42] make this view no longer tenable. In view of the question as to whether proteins are being turned over during the logarithmic growth of the cells it would be of interest to ascertain whether the rate constant for enzyme degradation of neo plastic tissues is similar to that of normal tissues.…”

Section: Introductionmentioning

confidence: 99%

The Roles of Synthesis and Degradation in the Regulation of Catalase Levels in the Neoplastic Tissues

Rechcigl¹,

Hruban²,

Hp³

1969

Enzymol Biol Clin

View full text Add to dashboard Cite

Summary. Several rat hepatomas were analyzed as to their catalase activity. Ample enzyme activity was found in each of the following tumors : the HC-hepatoma and hepatomas No. 7316, 7794, 7800, H-139, and H-122. Moderate activity was also seen in the LC-hepatoma, in various lines of 5123 hepatoma, and in hepatomas No. H-35, 7288C, 7787, 7793, 7795, 9098, and 9108. The rapidly growing hepatomas, such as the Novikoff, and hepatomas No. 3683, and 3924A were virtually devoid of any activity. Attempts have been made at the measurement of the rate of catalase synthesis and degradation in neoplastic tissues, as well as in the tissues of the tumor-bearing hosts. Advantage was taken of the methods elaborated in our laboratory, based on the novel properties of certain inhibitors to irreversibly inactivate catalase, or to block its synthesis. Using 5123 hepatoma, it was determined that during each hour 3.88 units of catalase were being synthesized per gram of the host liver, as compared to 0.92 units in the tumor. During the same one-hour period 2.4% of the catalase molecules present were being destroyed in both types of tissues. In the case of 7316A hepatoma, there were only 2.30 units catalase formed in an hour which can be contrasted with 4.00 units in the host liver. The rates of destruction in the respective tissues were 2.6 and 1.9% per h. The kinetic studies endeavored in some of the other tumors, particularly the HC-hepatoma, were largely unsuccessful because of their failure to respond to the inhibitors. The lack of response to 3-amino-l,2,4-triazole may be related to absence or low activity of hydrogen peroxide producing oxidases within microbodies, absence of microbodies, or to low permeability of the microbody (or the cell) to the drug. Hepatoma 7794A does not contain typical microbodies.

show abstract

“…Intermediate-sized filaments have been observed by electron microscopy in diverse types of liver tumors (31)(32)(33)(34)(35)(36). In our study they were especially abundant in the cytoplasm of cells of angiosarcomas (Fig.…”

Section: Resultsmentioning

confidence: 99%

Liver tumors distinguished by immunofluorescence microscopy with antibodies to proteins of intermediate-sized filaments.

Bannasch¹,

Zerban²,

Schmid³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Antibodies against constitutive proteins of different types of intermediate-sized filaments were used in immunofluorescence microscopy on frozen sections of normal rat liver and various rat liver tumors induced by treatment with nitrosamines. Antibodies to tonofilament prekeratin stained bile duct epithelia and hepatocytes of normal liver and hepatocellular carcinoma cells and ductal cells of cholangiofibromas. These cells were not significantly stained by antibodies to vimentin. By contrast, antibodies to vimentin stained mesenchymal cells of normal liver and cells of early and advanced angiosarcomas and of undifferentiated spindle cell sarcoma. These mesenchymal tumor cells were not stained with antibodies to prekeratin. (5,6,8,(11)(12)(13) has stimulated our interest in the possible maintenance of such molecular markers in tumors grown in the body.As a first example we have used the liver (in which different types of tumors, such as adenomas, hepatocellular carcinomas, cholangiofibromas, cholangiocarcinomas, angiosarcomas, and fibrosarcomas can occur) to determine whether epithelial and mesenchymal tumors could be distinguished by the use of antibodies to different filament proteins. The search for a molecular marker for identification of mesenchymal tumors of liver was especially desirable because mesenchymally derived liver tumors are important in human (14) and experimental animal (15) pathology. Reports of a high incidence of angiosarcomas in workers exposed to inorganic arsenic and gaseous vinyl chloride and in patients treated with Thorotrast or certain steroid hormones have increased the interest in this tumor (14, 16). The structure and morphogenesis of vascular liver tumors in humans and experimental animals appear to be largely identical (15,17). In both humans and animals, differential diagnosis of epithelial and mesenchymal liver tumors is sometimes difficult. A clear distinction, however, would be important not only for prognostic appraisals in human pathology but also for evaluation of animal experiments such as dose-response studies of carcinogenesis induced by chemical compounds (18, 19).In this study we show that immunofluorescence microscopy with antibodies to two molecular markers, prekeratin and vimentin, allows the character and origin of epithelial and mesenchymal cells to be distinguished in neoplastic liver cells and thus facilitates the differential diagnosis of liver tumors.MATERIALS AND METHODS Animals. Liver tumors were induced in male SpragueDawley rats (n-200 g body weight at the beginning of treatment) by oral treatment with nitrosamines (for methods, see ref. 20). Seven hepatocellular carcinomas, 3 cholangiofibromas, 10 angiosarcomas, and 1 undifferentiated spindle cell sarcoma were examined. Four of the hepatocellular carcinomas had developed in animals treated with 50 mg of N-nitrosomorpholine (in a total of 100 ml of their drinking water) for 3 weeks and killed between weeks 52 and 77 after withdrawal of the carcinogen. (Three of these carcinomas occurred in asso...

show abstract

Fine Structure of Transplantable Hepatomas of the Rat2

Cited by 91 publications

References 0 publications

Cytochemical Localization of Peroxidatic Activity of Catalase in Rat Hepatic Microbodies (Peroxisomes)

Cytochemical Localization of Peroxidatic Activity of Catalase in Rat Hepatic Microbodies (Peroxisomes)

The Roles of Synthesis and Degradation in the Regulation of Catalase Levels in the Neoplastic Tissues

Liver tumors distinguished by immunofluorescence microscopy with antibodies to proteins of intermediate-sized filaments.

Contact Info

Product

Resources

About