Fine‐tuning ethanol oxidation pathway enzymes and cofactor PQQ coordinates the conflict between fitness and acetic acid production by <i>Acetobacter pasteurianus</i>

Gao, Ling; Wu, Xiao‐Tao; Xia, Xiaole; Jin, Zhengyu

doi:10.1111/1751-7915.13703

Cited by 11 publications

(3 citation statements)

References 54 publications

(75 reference statements)

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“…According to Merli et al. (2021) , the growth of AAB typically decelerates at acetic acid concentrations above 40 g/L; however, certain strains, such as A. pasteurianus , exhibit resistance to these elevated concentrations, a tolerance essential for efficient ethanol oxidation ( Han et al., 2020 ; Gao et al., 2021 ). The present study used a high initial acetic acid concentration of 45 g/L, and the obtained acetification rate of 1.13 ± 0.05 g/L d corroborates the acidity resistance of A. pasteurianus ; this rate is comparable to values reported in similar studies ( Hidalgo et al., 2010 ; Qi et al., 2013 ).…”

Section: Resultsmentioning

confidence: 99%

Enhancing small-scale acetification processes using adsorbed Acetobacter pasteurianus UMCC 2951 on κ-carrageenan-coated luffa sponge

Sriphochanart,

Krusong,

Samuela

et al. 2024

PeerJ

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Background This study explored the utilization of luffa sponge (LS) in enhancing acetification processes. LS is known for having high porosity and specific surface area, and can provide a novel means of supporting the growth of acetic acid bacteria (AAB) to improve biomass yield and acetification rate, and thereby promote more efficient and sustainable vinegar production. Moreover, the promising potential of LS and luffa sponge coated with κ-carrageenan (LSK) means they may represent effective alternatives for the co-production of industrially valuable bioproducts, for example bacterial cellulose (BC) and acetic acid. Methods LS and LSK were employed as adsorbents for Acetobacter pasteurianus UMCC 2951 in a submerged semi-continuous acetification process. Experiments were conducted under reciprocal shaking at 1 Hz and a temperature of 32 °C. The performance of the two systems (LS-AAB and LSK-AAB respectively) was evaluated based on cell dry weight (CDW), acetification rate, and BC biofilm formation. Results The use of LS significantly increased the biomass yield during acetification, achieving a CDW of 3.34 mg/L versus the 0.91 mg/L obtained with planktonic cells. Coating LS with κ-carrageenan further enhanced yield, with a CDW of 4.45 mg/L. Acetification rates were also higher in the LSK-AAB system, reaching 3.33 ± 0.05 g/L d as opposed to 2.45 ± 0.05 g/L d for LS-AAB and 1.13 ± 0.05 g/L d for planktonic cells. Additionally, BC biofilm formation during the second operational cycle was more pronounced in the LSK-AAB system (37.0 ± 3.0 mg/L, as opposed to 25.0 ± 2.0 mg/L in LS-AAB). Conclusions This study demonstrates that LS significantly improves the efficiency of the acetification process, particularly when enhanced with κ-carrageenan. The increased biomass yield, accelerated acetification, and enhanced BC biofilm formation highlight the potential of the LS-AAB system, and especially the LSK-AAB variant, in sustainable and effective vinegar production. These systems offer a promising approach for small-scale, semi-continuous acetification processes that aligns with eco-friendly practices and caters to specialized market needs. Finally, this innovative method facilitates the dual production of acetic acid and bacterial cellulose, with potential applications in biotechnological fields.

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Section: Resultsmentioning

confidence: 99%

Enhancing small-scale acetification processes using adsorbed Acetobacter pasteurianus UMCC 2951 on κ-carrageenan-coated luffa sponge

Sriphochanart,

Krusong,

Samuela

et al. 2024

PeerJ

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“…Metabolic flux analysis of the pathway from ethanol and glucose revealed that overproduction of the PQQ-dependent ADH is an effective way to improve the ethanol-oxidizing respiratory chain. pBBR1MCS-2 was used in A. pasteurianus to express genes of the membrane-bound alcohol dehydrogenase (adhA) and aldehyde dehydrogenase (aldH), PQQ biosynthesis genes (pqqAB or pqqABCDE), and combinations thereof, each under the control of the promoter P tuf that was 1.8-fold stronger than P adhA in GFP reporter assays (Gao et al 2020a). Synergistic expression of these genes could not only efficiently relieve the conflict between increased acetic acid production (69 g/L in semi-continuous cultivation) and compromised cell fitness, b ut al s o e nh an c ed th e a ce t i c a ci d t o l er an c e of A. pasteurianus to a high initial concentration of 3% (v/v) thereby shortening the duration of the starting-up process from 116 to 99 h. This strategy is of significance for decreasing costs for producing high-strength acetic acid industrially and will also be useful for the production of other desired organic acids, especially those involving PQQ-dependent enzymes.…”

Section: Other Expression Plasmids Used In Acetobactermentioning

confidence: 99%

On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases

Fricke

Klemm

Bott

et al. 2021

Appl Microbiol Biotechnol

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Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is accompanied by growing demands for metabolic engineering of AAB to take advantage of their properties and to improve their biomanufacturing efficiencies. Controlled expression of target genes is key to fundamental and applied microbiological research. In order to get an overview of expression systems and their applications in AAB, we carried out a comprehensive literature search using the Web of Science Core Collection database. The Acetobacteraceae family currently comprises 49 genera. We found overall 6097 publications related to one or more AAB genera since 1973, when the first successful recombinant DNA experiments in Escherichia coli have been published. The use of plasmids in AAB began in 1985 and till today was reported for only nine out of the 49 AAB genera currently described. We found at least five major expression plasmid lineages and a multitude of further expression plasmids, almost all enabling only constitutive target gene expression. Only recently, two regulatable expression systems became available for AAB, an N-acyl homoserine lactone (AHL)-inducible system for Komagataeibacter rhaeticus and an l-arabinose-inducible system for Gluconobacter oxydans. Thus, after 35 years of constitutive target gene expression in AAB, we now have the first regulatable expression systems for AAB in hand and further regulatable expression systems for AAB can be expected. Key points • Literature search revealed developments and usage of expression systems in AAB. • Only recently 2 regulatable plasmid systems became available for only 2 AAB genera. • Further regulatable expression systems for AAB are in sight.

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“…Initially, ADH initiates the conversion of ethanol to acetaldehyde by removing hydrogen atoms, leading to the production of acetaldehyde and the reduction of NAD+ to NADH. Acting as a catalyst, ADH accelerates ethanol oxidation and facilitates NAD+ reduction (Gao et al, 2021 ; Zakhari, 2006 ). Subsequently, ALDH plays a crucial role by catalysing the further oxidation of acetaldehyde to acetic acid through hydrogen atom elimination (Miah et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Acetobacter pasteurianus BP2201 alleviates alcohol‐induced hepatic and neuro‐toxicity and modulate gut microbiota in mice

Wen,

Wang,

Liu

et al. 2023

Microbial Biotechnology

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The excessive consumption of alcohol results in a dysbiosis of the gut microbiota, which subsequently impairs the gut microbiota‐brain/liver axes and induces cognitive dysfunction and hepatic injury. This study aimed to investigate the potential effect of Acetobacter pasteurianus BP2201 in reducing the negative effects of alcohol consumption on cognitive function and liver health by modulating the gut microbiota‐brain/liver axes. Treatment with A. pasteurianus BP2201 improved alcohol‐induced hippocampal damage, suppressed neuroinflammation, promoted neuroprotein expression in the hippocampus and enhanced cognitive function. At the same time, A. pasteurianus BP2201 can also reduce serum lipid levels, relieve oxidative stress, inhibit TLR4/MyD88/NF‐κB pathway, reduce the secretion of TNF‐α and IL‐1β, so as to improve alcoholic liver injury. Concomitantly, the treatment with A. pasteurianus BP2201 leads to a shift in the intestinal microbiota structure towards that of healthy individuals, inhibiting the proliferation of harmful bacteria and promoting the recovery of beneficial bacteria. In addition, it also improves brain cognitive dysfunction and liver health by affecting the gut microbiota‐brain/liver axes by promoting the synthesis of relevant amino acids and the metabolism of nucleotide base components. These findings demonstrate the potential of regulating the gut microbiome and gut microbiota‐brain/liver axes to mitigate alcohol‐induced disease.

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Fine‐tuning ethanol oxidation pathway enzymes and cofactor PQQ coordinates the conflict between fitness and acetic acid production by Acetobacter pasteurianus

Cited by 11 publications

References 54 publications

Enhancing small-scale acetification processes using adsorbed Acetobacter pasteurianus UMCC 2951 on κ-carrageenan-coated luffa sponge

Enhancing small-scale acetification processes using adsorbed Acetobacter pasteurianus UMCC 2951 on κ-carrageenan-coated luffa sponge

On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases

Acetobacter pasteurianus BP2201 alleviates alcohol‐induced hepatic and neuro‐toxicity and modulate gut microbiota in mice

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