2020
DOI: 10.1021/acssynbio.0c00405
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Fine-Tuning Multi-Gene Clusters via Well-Characterized Gene Expression Regulatory Elements: Case Study of the Arginine Synthesis Pathway in C. glutamicum

Abstract: Promoters and ribosome binding sites (RBSs) are routinely applied in gene expression regulation, but their orthogonality and combinatorial effects have not yet been systematically studied in Corynebacterium glutamicum. Here, 17 core promoters and 29 RBSs in C. glutamicum were characterized, which exhibited 470-fold and 430-fold in transcriptional and translational activity, respectively. By comparing the expression of two reporter genes regulated by multiple RBSs, the RBS efficacy showed significant dependence… Show more

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Cited by 21 publications
(23 citation statements)
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“…However, the excessive increase of gene expression leads to the problems of slow cell growth and low utilization of intermediate metabolites. In recent years, synthetic biology methods have been used to fine-tune gene expression to balance intracellular metabolic flow and thus maximize the yield of target products . The engineered strain with a high riboflavin titer was successfully constructed by the TIGR approach, which confirmed the application potential of synthetic biology in the riboflavin industry.…”
Section: Results and Discussionmentioning
confidence: 70%
“…However, the excessive increase of gene expression leads to the problems of slow cell growth and low utilization of intermediate metabolites. In recent years, synthetic biology methods have been used to fine-tune gene expression to balance intracellular metabolic flow and thus maximize the yield of target products . The engineered strain with a high riboflavin titer was successfully constructed by the TIGR approach, which confirmed the application potential of synthetic biology in the riboflavin industry.…”
Section: Results and Discussionmentioning
confidence: 70%
“…In addition, the RBS engineering was widely used to regulate the gene translation and related enzyme activity, to increase the yield of the target product in C. glutamicum . Bicistron-modified RBSs were developed in C. glutamicum to minimize the context dependency of RBSs, which has been applied to regulate the arginine synthesis pathway . Additionally, the sequence between RBS and the start codon of downstream genes also affected the expression of target genes significantly …”
Section: Synthetic Biological Device For Global Optimizationmentioning
confidence: 99%
“…28 Bicistronmodified RBSs were developed in C. glutamicum to minimize the context dependency of RBSs, which has been applied to regulate the arginine synthesis pathway. 29 Additionally, the sequence between RBS and the start codon of downstream genes also affected the expression of target genes significantly. 30 Except these traditional methods, the CRISPR interference (CRISPRi) technology has been widely used to regulate the expression of target genes.…”
Section: Synthetic Biological Device For Global Optimizationmentioning
confidence: 99%
“…25 Despite the excellent expression enabled by the integration of various modules, it remains challenging to design a one-for-all method that can accommodate the effective heterologous expression of proteins with different properties. 26,27 In the case of expression of ⊍-1,2-FUTs, it is still of great importance to specifically assemble gene expression regulation elements with high cooperative and minimum cell harmful actions to finely ameliorate its expression.…”
Section: Introductionmentioning
confidence: 99%
“…For example, studies have used codon optimization, culture conditions improvement, molecular chaperone and fusion tag to express the VenB and VenC, which were commonly regarded as hard‐characterized P450 enzymes 25 . Despite the excellent expression enabled by the integration of various modules, it remains challenging to design a one‐for‐all method that can accommodate the effective heterologous expression of proteins with different properties 26,27 . In the case of expression of α‐1,2‐FUTs, it is still of great importance to specifically assemble gene expression regulation elements with high cooperative and minimum cell harmful actions to finely ameliorate its expression.…”
Section: Introductionmentioning
confidence: 99%