Fingerprinting of cauliflower cultivars using RAPD markers

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.A R 0 3 0 1 2 F i n g e r p r i n t i n g c a u l i f l o w e r u s i n g R A P D m a r k e r s I . A . A s t a r i n i e t a l .

Morphological and Molecular Characterization of Oyster Mushroom (Pleurotus cystidiosus)

“…There is almost need of advance technique used for species identification beyond morphological and physiological criteria, because these characteristics are highly influenced by environmental conditions. The expression of particular gene is a cumulative outcome of environment and genetic makeup of a specie/strain (Kumar, 1999;Astarini et al, 2004). Biochemical markers can be a source to reflect the genetic study because they are Mushroom is a macro fungus with a distinctive fruiting body which can be either epigeous or hypogeous and large enough to be seen with naked eye and to be picked by hand.…”

Morphological and molecular characterization of Oyster mushroom (Pleurotus spp.)

“…The expression of particular gene is a cumulative outcome of environment and genetic makeup of a specie/strain (Kumar, 1999;Astarini et al, 2004). Biochemical markers can be a source to reflect the genetic variability because they are direct product of genes.…”

“…The genetic diversity of mushrooms has been worked out using molecular markers especially random amplified polymorphic DNA (RAPD) (Staniaszek et al, 2002: Yan et al, 2004Stajic et al, 2005;Ravash et al, 2009). RAPD is used to assess genetic similarity and phylogenetic analysis due to the simplicity in its methodology (Gepts, 1993).…”

High Levels of Genetic Variation as Detected by Aflp in Sideritis tmolea from Western Turkey

“…Traditionally, morphological traits, karyotypes and protein and isozyme markers have been widely used to assess genetic diversity (Mudibu et al, 2011). Therefore, the measurement of genetic variation based on morphological characteristics is time-consuming and incoherent and requires extensive field trials and evaluation (Astarini et al, 2004). Different DNA techniques (RFLP, RAPD, AFLP, SSR, CAPS, ISSR and SNP) that are not affected by the environment (Maciel et al, 2003) are necessary for genetic screening among categories of closely related species in germplasm banks (Lapitan et al, 2007).…”